Restriction map for
pFN21AB7616
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI AocI
ApoI AscI AseI AsnI Asp718I
AspEI AvaII AviII AvrII BamHI
BbrPI BbvCI BclI BfrI BglII
BlnI BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrGI BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DraIII DrdI EagI Eam1105I
EarI EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI HphI KpnI Ksp632I MaeIII
MamI MfeI MluI MroI MunI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI RcaI
RsrII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tsp45I Tsp509I
Van91I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 520 GACGTC GACGT^C
Alw44I 84 GTGCAC G^TGCAC
AlwI 108 GATCC GGATC(4/5)
AlwNI 823 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 84 GTGCAC G^TGCAC
Asp700I 492 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 966 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 70 GGCGCC G^G[CT][GA]CC
BbeI 70 GGCGCC GGCGC^C
BciVI 572 GGATAC GTATCC(6/5)
BfaI 478 CTAG C^TAG
BglI 240 GCCCGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsiEI 393 CGATCG CG[GA][CT]^CG
BsmAI 43 GAGAC GTCTC(1/5)
BsmFI 964 GGGAC GGGAC(10/14)
BsmI 496 GCATTC GAATGC(1/-1)
BsrBI 859 GAGCGG CCGCTC(-3/-3)
BsrDI 915 CATTGC GCAATG(2/0)
BsrFI 1041 GCCGGC [GA]^CCGG[CT]
BssHII 190 GCGCGC G^CGCGC
BstYI 107 AGATCC [GA]^GATC[CT]
Cfr10I 1041 GCCGGC [GA]^CCGG[CT]
DraI 417 TTTAAA TTT^AAA
KasI 70 GGCGCC G^GCGCC
MaeI 478 CTAG C^TAG
MaeII 521 ACGT A^CGT
MflI 107 AGATCC [GA]^GATC[CT]
MseI 418 TTAA T^TAA
NaeI 1041 GCCGGC GCC^GGC
NarI 70 GGCGCC GG^CGCC
NgoMIV 1041 GCCGGC G^CCGGC
PflFI 966 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 393 CGATCG CGAT^CG
PvuII 1066 CAGCTG CAG^CTG
SacI 135 GAGCTC GAGCT^C
SfoI 70 GGCGCC GGC^GCC
SmlI 709 CTCAAG C^T[CT][GA]AG
TfiI 370 GATTC G^A[AT]TC
Tru9I 418 TTAA T^TAA
Tth111I 966 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 107 AGATCC [GA]^GATC[CT]
XmnI 492 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 70 GGCGCC G[GA]^CG[CT]C
520 GACGTC
AfaI 59 GTAC GT^AC
998 GTAC
Aor51HI 21 AGCGCT AGC^GCT
153 AGCGCT
ApaI 238 GGGCCC GGGCC^C
864 GGGCCC
BalI 50 TGGCCA TGG^CCA
542 TGGCCA
BbsI 565 GAAGAC GAAGAC(2/6)
1033 GAAGAC
BcgI 86 GCACTGGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
787 GCAGCCGAGTCG
BlpI 24 GCTGAGC GC^T[ACGT]AGC
201 GCTCAGC
Bpu1102I 24 GCTGAGC GC^T[ACGT]AGC
201 GCTCAGC
BpuAI 565 GAAGAC GAAGAC(2/6)
1033 GAAGAC
BsaHI 70 GGCGCC G[GA]^CG[CT]C
520 GACGTC
BsrI 88 ACTGG ACTGG(1/-1)
756 ACTGG
BstXI 272 CCACCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
953 CCAAAGTGCTGG
CelII 24 GCTGAGC GC^T[ACGT]AGC
201 GCTCAGC
DpnII 108 GATC ^GATC
394 GATC
Eco47III 21 AGCGCT AGC^GCT
153 AGCGCT
HgaI 871 GACGC GACGC(5/10)
305 GCGTC
Hin1I 70 GGCGCC G[GA]^CG[CT]C
520 GACGTC
KspI 145 CCGCGG CCGC^GG
209 CCGCGG
MboI 108 GATC ^GATC
394 GATC
MluNI 50 TGGCCA TGG^CCA
542 TGGCCA
MscI 50 TGGCCA TGG^CCA
542 TGGCCA
MslI 915 CATTGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
951 CACCAAAGTG
NcoI 671 CCATGG C^CATGG
1025 CCATGG
NdeII 108 GATC ^GATC
394 GATC
RsaI 59 GTAC GT^AC
998 GTAC
SacII 145 CCGCGG CCGC^GG
209 CCGCGG
Sau3AI 108 GATC ^GATC
394 GATC
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AccII AciI AluI AspHI AvaI
BanII BbvI BcnI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsoBI Bsp1286I BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIII NlaIV PleI Sau96I ScrFI
SfaNI SmaI StyI TaqI TseI
TspRI TthHB8I XmaCI XmaI