Restriction map for
pFN21AE6633
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI AlwI AocI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbrPI
BbsI BcgI BfrI BglI BglII
BlnI BpuAI BsaAI BsaBI BsaI
BseAI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstUI BstXI BstYI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraIII DsaI EagI Eam1105I EclXI
Eco52I Eco81I EcoRI EcoRV EcoT22I
FseI FspI HindIII HpaI KpnI
KspI MaeII MamI MfeI MflI
MluI MroI MslI MunI MvnI
NaeI NcoI NdeI NgoMIV NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI SexAI SfaNI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tth111I TthHB8I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Alw44I 682 GTGCAC G^TGCAC
Aor51HI 575 AGCGCT AGC^GCT
ApaI 878 GGGCCC GGGCC^C
ApaLI 682 GTGCAC G^TGCAC
AvaI 191 CCCGAG C^[CT]CG[GA]G
BbeI 136 GGCGCC GGCGC^C
BciVI 255 GGATAC GTATCC(6/5)
BcnI 662 CCGGG CC^[GC]GG
BlpI 898 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 898 GCTGAGC GC^T[ACGT]AGC
BsaWI 938 TCCGGT [AT]^CCGG[AT]
BsmBI 160 GAGACG CGTCTC(1/5)
BsmFI 15 GGGAC GGGAC(10/14)
BsoBI 191 CCCGAG C^[CT]CG[GA]G
BspMI 467 ACCTGC ACCTGC(4/8)
BsrBI 699 CCGCTC CCGCTC(-3/-3)
BssSI 632 CTCGTG CACGAG(-5/-1)
BstF5I 59 CATCC GGATG(2/0)
CelII 898 GCTGAGC GC^T[ACGT]AGC
DrdI 861 GACCTGGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 748 CTCTTC CTCTTC(1/4)
Eco47III 575 AGCGCT AGC^GCT
EcoNI 807 CCTGGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 376 GCGGG CCCGC(4/6)
FokI 59 CATCC GGATG(9/13)
HgaI 692 GCGTC GACGC(5/10)
HincII 436 GTCAAC GT[CT]^[GA]AC
HindII 436 GTCAAC GT[CT]^[GA]AC
KasI 136 GGCGCC G^GCGCC
Ksp632I 748 CTCTTC CTCTTC(1/4)
MseI 512 TTAA T^TAA
NarI 136 GGCGCC GG^CGCC
NciI 662 CCGGG CC^[GC]GG
NheI 178 GCTAGC G^CTAGC
PflMI 270 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 859 AGGACCT [GA]G^G[AT]CC[CT]
PstI 207 CTGCAG CTGCA^G
SacI 172 GAGCTC GAGCT^C
SapI 747 GCTCTTC GCTCTTC(1/4)
ScaI 839 AGTACT AGT^ACT
SfoI 136 GGCGCC GGC^GCC
Tru9I 512 TTAA T^TAA
Tsp509I 287 AATT ^AATT
Van91I 270 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 136 GGCGCC G[GA]^CG[CT]C
691 GGCGTC
AfaI 217 GTAC GT^AC
840 GTAC
AlwNI 271 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
846 CAGTGACTG
AspHI 172 GAGCTC G[AT]GC[AT]^C
682 GTGCAC
BalI 614 TGGCCA TGG^CCA
641 TGGCCA
BanI 136 GGCGCC G^G[CT][GA]CC
658 GGCACC
BbvCI 325 GCTGAGG CCTCAGC(-5/-2)
811 GCTGAGG
BclI 128 TGATCA T^GATCA
365 TGATCA
BfaI 179 CTAG C^TAG
931 CTAG
BsaHI 136 GGCGCC G[GA]^CG[CT]C
691 GGCGTC
BsiHKAI 172 GAGCTC G[AT]GC[AT]^C
682 GTGCAC
BsmAI 160 GAGAC GTCTC(1/5)
870 GTCTC
BsrI 133 ACTGG ACTGG(1/-1)
239 CCAGT
BstEII 6 GGTCACC G^GT[ACGT]ACC
666 GGTCACC
BstPI 6 GGTCACC G^GT[ACGT]ACC
666 GGTCACC
DpnII 129 GATC ^GATC
366 GATC
EcoO65I 6 GGTCACC G^GT[ACGT]ACC
666 GGTCACC
FbaI 128 TGATCA T^GATCA
365 TGATCA
Hin1I 136 GGCGCC G[GA]^CG[CT]C
691 GGCGTC
MaeI 179 CTAG C^TAG
931 CTAG
MboI 129 GATC ^GATC
366 GATC
MboII 32 TCTTC GAAGA(8/7)
749 TCTTC
MluNI 614 TGGCCA TGG^CCA
641 TGGCCA
MscI 614 TGGCCA TGG^CCA
641 TGGCCA
NdeII 129 GATC ^GATC
366 GATC
RsaI 217 GTAC GT^AC
840 GTAC
Sau3AI 129 GATC ^GATC
366 GATC
SfcI 207 CTGCAG C^T[GA][CT]AG
579 CTACAG
TfiI 546 GATTC G^A[AT]TC
935 GAATC
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# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AluI AvaII BanII BbvI
BmyI BpmI BsaJI BseRI BsgI
BsiYI BslI Bsp1286I BssKI BstNI
Cac8I CfoI Cfr13I DdeI DraII
EaeI EcoO109I EcoRII EcoT14I Fnu4HI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MnlI MspA1I MspI MvaI MwoI
NlaIV PleI PvuII Sau96I ScrFI
StyI TseI Tsp45I TspRI