Restriction map for
pFN21AB8933
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspI AviII
AvrII BalI BamHI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraII DraIII DrdI
Eco47III Eco81I EcoO109I EcoO65I EcoRV
EcoT22I FbaI FokI FseI FspI
HgaI HincII HindII HindIII HpaI
KpnI MaeI MamI MfeI MluI
MluNI MroI MscI MslI MunI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SalI SapI SbfI ScaI
SexAI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tth111I Van91I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcyI 310 GGCGCC G[GA]^CG[CT]C
AhdI 841 GACTTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 204 GATCC GGATC(4/5)
AspEI 841 GACTTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 744 CCCGGG C^[CT]CG[GA]G
BanI 310 GGCGCC G^G[CT][GA]CC
BbeI 310 GGCGCC GGCGC^C
BglI 541 GCCGCGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 274 TACGTG [CT]AC^GT[GA]
BsaHI 310 GGCGCC G[GA]^CG[CT]C
BsaWI 521 ACCGGA [AT]^CCGG[AT]
BsgI 127 CTGCAC GTGCAG(16/14)
BsiEI 297 CGGCCG CG[GA][CT]^CG
BsmFI 365 GGGAC GGGAC(10/14)
BsoBI 744 CCCGGG C^[CT]CG[GA]G
BsrBI 631 CCGCTC CCGCTC(-3/-3)
BsrFI 481 ACCGGC [GA]^CCGG[CT]
BstYI 203 AGATCC [GA]^GATC[CT]
Cfr10I 481 ACCGGC [GA]^CCGG[CT]
DdeI 645 CTCAG C^T[ACGT]AG
DpnII 204 GATC ^GATC
EagI 297 CGGCCG C^GGCCG
Eam1105I 841 GACTTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 649 GAAGAG CTCTTC(1/4)
EclXI 297 CGGCCG C^GGCCG
Eco52I 297 CGGCCG C^GGCCG
EcoNI 613 CCTCTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 655 GAATTC G^AATTC
Hin1I 310 GGCGCC G[GA]^CG[CT]C
HinfI 805 GACTC G^A[ACGT]TC
KasI 310 GGCGCC G^GCGCC
Ksp632I 649 GAAGAG CTCTTC(1/4)
MaeII 275 ACGT A^CGT
MboI 204 GATC ^GATC
MboII 649 GAAGA GAAGA(8/7)
MflI 203 AGATCC [GA]^GATC[CT]
NarI 310 GGCGCC GG^CGCC
NdeII 204 GATC ^GATC
NlaIII 636 CATG CATG^
PleI 805 GACTC GAGTC(4/5)
Sau3AI 204 GATC ^GATC
SfiI 540 GGCCGCGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 310 GGCGCC GGC^GCC
SmaI 744 CCCGGG CCC^GGG
SmlI 148 CTCAAG C^T[CT][GA]AG
XhoII 203 AGATCC [GA]^GATC[CT]
XmaCI 744 CCCGGG C^CCGGG
XmaI 744 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcsI 340 AAATTT [GA]^AATT[CT]
655 GAATTC
AfaI 145 GTAC GT^AC
426 GTAC
ApoI 340 AAATTT [GA]^AATT[CT]
655 GAATTC
AspHI 193 GAGCTC G[AT]GC[AT]^C
726 GAGCTC
AvaII 690 GGTCC G^G[AT]CC
848 GGTCC
BanII 193 GAGCTC G[GA]GC[CT]^C
726 GAGCTC
BmyI 193 GAGCTC G[AGT]GC[ACT]^C
726 GAGCTC
BsaI 376 GAGACC GGTCTC(1/5)
533 GAGACC
BsiHKAI 193 GAGCTC G[AT]GC[AT]^C
726 GAGCTC
BsmAI 376 GAGAC GTCTC(1/5)
533 GAGAC
Bsp1286I 193 GAGCTC G[AGT]GC[ACT]^C
726 GAGCTC
BspMI 125 ACCTGC ACCTGC(4/8)
92 GCAGGT
BstXI 155 CCAAGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
830 CCAGTCTGGTGG
EaeI 297 CGGCCG [CT]^GGCC[GA]
470 TGGCCG
EcoT14I 155 CCAAGG C^C[AT][AT]GG
551 CCAAGG
HaeII 83 AGCGCC [GA]GCGC^[CT]
310 GGCGCC
KspI 177 CCGCGG CCGC^GG
542 CCGCGG
MaeIII 105 GTCAC ^GT[ACGT]AC
770 GTCAC
MseI 515 TTAA T^TAA
751 TTAA
MspA1I 177 CCGCGG C[AC]G^C[GT]G
542 CCGCGG
RsaI 145 GTAC GT^AC
426 GTAC
SacI 193 GAGCTC GAGCT^C
726 GAGCTC
SacII 177 CCGCGG CCGC^GG
542 CCGCGG
SfaNI 6 GATGC GCATC(5/9)
792 GCATC
StyI 155 CCAAGG C^C[AT][AT]GG
551 CCAAGG
TaqI 4 TCGA T^CGA
403 TCGA
Tru9I 515 TTAA T^TAA
751 TTAA
Tsp45I 105 GTCAC ^GT[GC]AC
770 GTCAC
Tsp509I 341 AATT ^AATT
656 AATT
TspRI 107 CACTG CA[GC]TG[ACGT][ACGT]^
347 CACTG
TthHB8I 4 TCGA T^CGA
403 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AccII AciI AluI AlwNI BbvI
BcnI BpmI BsaJI BseRI BsiYI
BslI BsrI BssKI BstNI BstUI
Cac8I CfoI Cfr13I DsaI EcoRII
FauI Fnu4HI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MnlI
MspI MvaI MvnI MwoI NciI
NlaIV PstI Sau96I ScrFI SfcI
TseI