Restriction map for
pFN21AE0912
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI AspEI AspI AvaI AviII
AvrII BalI BbeI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BseAI BseRI BsgI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI Eam1105I Eco47III
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FokI FseI FspI HincII
HindII HindIII HpaI KasI KspI
MaeI MamI MfeI MluI MluNI
MroI MscI MseI MslI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NlaIII NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI ScaI SexAI
SfaNI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI SspBI
SspI StuI SwaI TaqI TfiI
Tru9I Tth111I TthHB8I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 437 GGTACC G^GTACC
AcsI 21 GAATTT [GA]^AATT[CT]
AcyI 649 GGCGTC G[GA]^CG[CT]C
ApoI 21 GAATTT [GA]^AATT[CT]
Asp700I 348 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 437 GGTACC G^GTACC
BamHI 447 GGATCC G^GATCC
BanI 437 GGTACC G^G[CT][GA]CC
BpmI 574 CTCCAG CTGGAG(16/14)
BsaHI 649 GGCGTC G[GA]^CG[CT]C
BsaI 55 GAGACC GGTCTC(1/5)
BsaWI 493 ACCGGA [AT]^CCGG[AT]
BsiEI 665 CGGCCG CG[GA][CT]^CG
BsmAI 55 GAGAC GTCTC(1/5)
BssSI 167 CACGAG CACGAG(-5/-1)
BstYI 447 GGATCC [GA]^GATC[CT]
DdeI 296 CTGAG C^T[ACGT]AG
DpnII 448 GATC ^GATC
DsaI 113 CCGTGG C^C[GA][CT]GG
EagI 665 CGGCCG C^GGCCG
EarI 64 GAAGAG CTCTTC(1/4)
EclXI 665 CGGCCG C^GGCCG
Eco52I 665 CGGCCG C^GGCCG
EcoNI 588 CCTGTCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 239 CCAAGG C^C[AT][AT]GG
HgaI 650 GCGTC GACGC(5/10)
Hin1I 649 GGCGTC G[GA]^CG[CT]C
HphI 236 TCACC GGTGA(8/7)
KpnI 437 GGTACC GGTAC^C
Ksp632I 64 GAAGAG CTCTTC(1/4)
MaeIII 591 GTCAC ^GT[ACGT]AC
MboI 448 GATC ^GATC
MflI 447 GGATCC [GA]^GATC[CT]
NdeII 448 GATC ^GATC
PflMI 33 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 7 CAGCTG CAG^CTG
Sau3AI 448 GATC ^GATC
SbfI 441 CCTGCAGG CCTGCA^GG
Sse8387I 441 CCTGCAGG CCTGCA^GG
StyI 239 CCAAGG C^C[AT][AT]GG
Tsp45I 591 GTCAC ^GT[GC]AC
Tsp509I 22 AATT ^AATT
TspRI 397 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 33 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 447 GGATCC [GA]^GATC[CT]
XmnI 348 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 336 GTAC GT^AC
438 GTAC
AlwI 448 GATCC GGATC(4/5)
447 GGATC
AlwNI 262 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
583 CAGGTCCTG
AspHI 205 GAGCTC G[AT]GC[AT]^C
478 GAGCTC
BanII 205 GAGCTC G[GA]GC[CT]^C
478 GAGCTC
BbsI 311 GAAGAC GAAGAC(2/6)
390 GAAGAC
BpuAI 311 GAAGAC GAAGAC(2/6)
390 GAAGAC
BsiHKAI 205 GAGCTC G[AT]GC[AT]^C
478 GAGCTC
BsrBI 69 GAGCGG CCGCTC(-3/-3)
551 GAGCGG
BsrI 521 ACTGG ACTGG(1/-1)
671 CCAGT
FauI 357 CCCGC CCCGC(4/6)
606 GCGGG
HaeII 83 AGCGCC [GA]GCGC^[CT]
264 GGCGCT
MaeII 474 ACGT A^CGT
712 ACGT
MspA1I 7 CAGCTG C[AC]G^C[GT]G
358 CCGCTG
PpuMI 321 GGGACCT [GA]G^G[AT]CC[CT]
584 AGGTCCT
RsaI 336 GTAC GT^AC
438 GTAC
SacI 205 GAGCTC GAGCT^C
478 GAGCTC
SfiI 117 GGCCCCTGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
126 GGCCTGCGAGGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AccII AciI AluI AvaII BbvI
BcnI BglI BmyI BsaJI BsiYI
BslI BsmFI Bsp1286I BspMI BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DraII EaeI EcoO109I EcoRII Fnu4HI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MboII MnlI MspI
MvaI MvnI MwoI NciI NlaIV
PleI PstI Sau96I ScrFI SfcI
TseI