##################################
# NO CUT ENZYMES (  206 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        AluI        Alw44I      
AlwI        AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BcnI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaWI       
BseAI       BseRI       BsiEI       BsiHKAI     BsiWI       
BsiYI       BslI        BsmBI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstF5I      BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      Cac8I       CelII       
CfoI        Cfr10I      ClaI        CpoI        DdeI        
DraIII      DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FauI        
FokI        FseI        FspI        HaeII       HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        HpaII       HphI        
KasI        KpnI        KspI        MaeI        MaeII       
MaeIII      MamI        MfeI        MflI        MluI        
MnlI        MroI        MslI        MspA1I      MspI        
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfaNI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       StuI        SwaI        TaqI        Tsp45I      
TspRI       Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AcsI           46  GAATTT               [GA]^AATT[CT]
AlwNI          17  CAGAATCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI           46  GAATTT               [GA]^AATT[CT]
AvaII          56  GGACC                G^G[AT]CC
BalI          255  TGGCCA               TGG^CCA
BclI           24  TGATCA               T^GATCA
BsgI            5  CTGCAC               GTGCAG(16/14)
BsmFI          55  GGGAC                GGGAC(10/14)
BspMI          79  ACCTGC               ACCTGC(4/8)
BsrDI         151  CATTGC               GCAATG(2/0)
BssKI         231  CCTGG                ^CC[ACGT]GG
BstNI         231  CCTGG                CC^[AT]GG
Cfr13I         56  GGACC                G^G[ACGT]CC
DpnII          25  GATC                 ^GATC
DraI          168  TTTAAA               TTT^AAA
DraII          55  GGGACCT              [GA]G^G[ACGT]CC[CT]
EaeI          255  TGGCCA               [CT]^GGCC[GA]
EarI          130  GAAGAG               CTCTTC(1/4)
EcoO109I       55  GGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRII        231  CCTGG                ^CC[AT]GG
EcoT14I       252  CCTTGG               C^C[AT][AT]GG
FbaI           24  TGATCA               T^GATCA
HaeIII        256  GGCC                 GG^CC
Ksp632I       130  GAAGAG               CTCTTC(1/4)
MboI           25  GATC                 ^GATC
MboII         130  GAAGA                GAAGA(8/7)
MluNI         255  TGGCCA               TGG^CCA
MscI          255  TGGCCA               TGG^CCA
MvaI          231  CCTGG                CC^[AT]GG
NdeII          25  GATC                 ^GATC
NlaIII        192  CATG                 CATG^
NlaIV          55  GGGACC               GG[ACGT]^[ACGT]CC
PleI          248  GACTC                GAGTC(4/5)
PpuMI          55  GGGACCT              [GA]G^G[AT]CC[CT]
Sau3AI         25  GATC                 ^GATC
Sau96I         56  GGACC                G^G[ACGT]CC
ScrFI         231  CCTGG                CC^[ACGT]GG
SspI          164  AATATT               AAT^ATT
StyI          252  CCTTGG               C^C[AT][AT]GG
TfiI           19  GAATC                G^A[AT]TC
Tsp509I        47  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

BbvI          235  GCAGC                GCAGC(8/12)
                4  GCTGC               
BsaI           76  GAGACC               GGTCTC(1/5)
              133  GAGACC              
BsaJI         230  CCCTGG               C^C[ACGT][ACGT]GG
              252  CCTTGG              
BsmAI          76  GAGAC                GTCTC(1/5)
              133  GAGAC               
Fnu4HI          4  GCTGC                GC^[ACGT]GC
              235  GCAGC               
HinfI          19  GAATC                G^A[ACGT]TC
              248  GACTC               
ItaI            4  GCTGC                GC^[ACGT]GC
              235  GCAGC               
MwoI          229  GCCCTGGCAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              235  GCAGCCAAAGC         
TseI            4  GCTGC                G^C[AT]GC
              235  GCAGC               


#########################################
# 3 OR MORE CUT ENZYMES (    2 enzymes) #
#########################################

MseI        Tru9I