################################## # NO CUT ENZYMES ( 206 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AfaI AflII AflIII AgeI AhdI AluI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DdeI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FokI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI KspI MaeI MaeII MaeIII MamI MfeI MflI MluI MnlI MroI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TaqI Tsp45I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AcsI 46 GAATTT [GA]^AATT[CT] AlwNI 17 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 46 GAATTT [GA]^AATT[CT] AvaII 56 GGACC G^G[AT]CC BalI 255 TGGCCA TGG^CCA BclI 24 TGATCA T^GATCA BsgI 5 CTGCAC GTGCAG(16/14) BsmFI 55 GGGAC GGGAC(10/14) BspMI 79 ACCTGC ACCTGC(4/8) BsrDI 151 CATTGC GCAATG(2/0) BssKI 231 CCTGG ^CC[ACGT]GG BstNI 231 CCTGG CC^[AT]GG Cfr13I 56 GGACC G^G[ACGT]CC DpnII 25 GATC ^GATC DraI 168 TTTAAA TTT^AAA DraII 55 GGGACCT [GA]G^G[ACGT]CC[CT] EaeI 255 TGGCCA [CT]^GGCC[GA] EarI 130 GAAGAG CTCTTC(1/4) EcoO109I 55 GGGACCT [GA]G^G[ACGT]CC[CT] EcoRII 231 CCTGG ^CC[AT]GG EcoT14I 252 CCTTGG C^C[AT][AT]GG FbaI 24 TGATCA T^GATCA HaeIII 256 GGCC GG^CC Ksp632I 130 GAAGAG CTCTTC(1/4) MboI 25 GATC ^GATC MboII 130 GAAGA GAAGA(8/7) MluNI 255 TGGCCA TGG^CCA MscI 255 TGGCCA TGG^CCA MvaI 231 CCTGG CC^[AT]GG NdeII 25 GATC ^GATC NlaIII 192 CATG CATG^ NlaIV 55 GGGACC GG[ACGT]^[ACGT]CC PleI 248 GACTC GAGTC(4/5) PpuMI 55 GGGACCT [GA]G^G[AT]CC[CT] Sau3AI 25 GATC ^GATC Sau96I 56 GGACC G^G[ACGT]CC ScrFI 231 CCTGG CC^[ACGT]GG SspI 164 AATATT AAT^ATT StyI 252 CCTTGG C^C[AT][AT]GG TfiI 19 GAATC G^A[AT]TC Tsp509I 47 AATT ^AATT ################################### # TWO CUT ENZYMES ( 9 enzymes) # ################################### BbvI 235 GCAGC GCAGC(8/12) 4 GCTGC BsaI 76 GAGACC GGTCTC(1/5) 133 GAGACC BsaJI 230 CCCTGG C^C[ACGT][ACGT]GG 252 CCTTGG BsmAI 76 GAGAC GTCTC(1/5) 133 GAGAC Fnu4HI 4 GCTGC GC^[ACGT]GC 235 GCAGC HinfI 19 GAATC G^A[ACGT]TC 248 GACTC ItaI 4 GCTGC GC^[ACGT]GC 235 GCAGC MwoI 229 GCCCTGGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 235 GCAGCCAAAGC TseI 4 GCTGC G^C[AT]GC 235 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 2 enzymes) # ######################################### MseI Tru9I