Restriction map for
pFN21AE3965
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# NO CUT ENZYMES ( 169 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BfaI BglI BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BseRI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII Cfr13I ClaI CpoI DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
HphI KasI KpnI KspI MaeI
MaeII MamI MfeI MluI MluNI
MroI MscI MspA1I MunI NaeI
NarI NdeI NgoMIV NheI NlaIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SapI Sau96I SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI TaqI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccII 54 CGCG CG^CG
AflII 673 CTTAAG C^TTAAG
Aor51HI 522 AGCGCT AGC^GCT
BbvI 4 GCTGC GCAGC(8/12)
BcnI 552 CCGGG CC^[GC]GG
BfrI 673 CTTAAG C^TTAAG
BglII 502 AGATCT A^GATCT
BmyI 912 GTGCCC G[AGT]GC[ACT]^C
BsaI 145 GAGACC GGTCTC(1/5)
BsaJI 575 CCATGG C^C[ACGT][ACGT]GG
BsgI 921 GTGCAG GTGCAG(16/14)
BsiYI 90 CCTGGTATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 90 CCTGGTATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 912 GTGCCC G[AGT]GC[ACT]^C
BspHI 666 TCATGA T^CATGA
BspMI 67 ACCTGC ACCTGC(4/8)
BsrDI 305 GCAATG GCAATG(2/0)
BsrFI 85 ACCGGC [GA]^CCGG[CT]
BstNI 90 CCTGG CC^[AT]GG
BstUI 54 CGCG CG^CG
BstXI 83 CCACCGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 502 AGATCT [GA]^GATC[CT]
Cac8I 923 GCAGGC GC[ACGT]^[ACGT]GC
CfoI 523 GCGC GCG^C
Cfr10I 85 ACCGGC [GA]^CCGG[CT]
DsaI 575 CCATGG C^C[GA][CT]GG
Eco47III 522 AGCGCT AGC^GCT
EcoRII 90 CCTGG ^CC[AT]GG
EcoT14I 575 CCATGG C^C[AT][AT]GG
HaeII 522 AGCGCT [GA]GCGC^[CT]
HaeIII 88 GGCC GG^CC
HhaI 523 GCGC GCG^C
HinP1I 523 GCGC G^CGC
MflI 502 AGATCT [GA]^GATC[CT]
MvaI 90 CCTGG CC^[AT]GG
MvnI 54 CGCG CG^CG
MwoI 914 GCCCAATGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 552 CCGGG CC^[GC]GG
NcoI 575 CCATGG C^CATGG
RcaI 666 TCATGA T^CATGA
SfaNI 382 GATGC GCATC(5/9)
StyI 575 CCATGG C^C[AT][AT]GG
TseI 4 GCTGC G^C[AT]GC
Tsp45I 79 GTGAC ^GT[GC]AC
XhoII 502 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 135 AAATTC [GA]^AATT[CT]
557 AAATTT
ApoI 135 AAATTC [GA]^AATT[CT]
557 AAATTT
BbsI 481 GAAGAC GAAGAC(2/6)
987 GAAGAC
BpuAI 481 GAAGAC GAAGAC(2/6)
987 GAAGAC
BsmAI 145 GAGAC GTCTC(1/5)
183 GAGAC
BsmFI 771 GGGAC GGGAC(10/14)
838 GGGAC
BssKI 90 CCTGG ^CC[ACGT]GG
552 CCGGG
Fnu4HI 4 GCTGC GC^[ACGT]GC
39 GCCGC
HapII 86 CCGG C^CGG
552 CCGG
HpaII 86 CCGG C^CGG
552 CCGG
ItaI 4 GCTGC GC^[ACGT]GC
39 GCCGC
MseI 425 TTAA T^TAA
674 TTAA
MslI 51 CATCGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
875 CATGCCTGTG
MspI 86 CCGG C^CGG
552 CCGG
PleI 773 GACTC GAGTC(4/5)
990 GACTC
PstI 331 CTGCAG CTGCA^G
621 CTGCAG
ScrFI 90 CCTGG CC^[ACGT]GG
552 CCGGG
SfcI 331 CTGCAG C^T[GA][CT]AG
621 CTGCAG
SmlI 653 CTCAAG C^T[CT][GA]AG
673 CTTAAG
SspI 627 AATATT AAT^ATT
638 AATATT
Tru9I 425 TTAA T^TAA
674 TTAA
TspRI 584 CAGTG CA[GC]TG[ACGT][ACGT]^
910 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AciI AfaI AluI BsrI BstF5I
DdeI DpnII EarI FokI HinfI
Ksp632I MaeIII MboI MboII MnlI
NdeII NlaIII NspI RsaI Sau3AI
TfiI Tsp509I