Restriction map for
pFN21AE2290
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# NO CUT ENZYMES ( 101 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AlwI
ApoI Asp700I Asp718I AspI AviII
AvrII BamHI BbsI BbvCI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaI
BsaWI BseAI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrGI BssSI
Bst1107I BstAPI BstBI BstYI BstZ17I
CelII ClaI DraI DraIII EcoNI
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MaeI MamI MfeI
MflI MluI MroI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI Psp1406I SacII SalI SbfI
SfuI SgrAI SmlI SnaBI SpeI
Sse8387I SspBI SspI SwaI TfiI
Tth111I XbaI XcmI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 72 enzymes) #
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AcyI 306 GGCGCC G[GA]^CG[CT]C
AhdI 19 GACTCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 324 GTGCAC G^TGCAC
AlwNI 289 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 951 CCTTAGG CC^T[ACGT]AGG
Aor51HI 398 AGCGCT AGC^GCT
ApaI 737 GGGCCC GGGCC^C
ApaLI 324 GTGCAC G^TGCAC
AscI 138 GGCGCGCC GG^CGCGCC
AseI 916 ATTAAT AT^TAAT
AsnI 916 ATTAAT AT^TAAT
AspEI 19 GACTCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 362 TGGCCA TGG^CCA
BbeI 306 GGCGCC GGCGC^C
BbrPI 202 CACGTG CAC^GTG
BglI 722 GCCGGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 394 CTGGAG CTGGAG(16/14)
BsaAI 202 CACGTG [CT]AC^GT[GA]
BsaHI 306 GGCGCC G[GA]^CG[CT]C
BseRI 772 GAGGAG GAGGAG(10/8)
BsgI 165 GTGCAG GTGCAG(16/14)
BsiWI 275 CGTACG C^GTACG
BspHI 803 TCATGA T^CATGA
BsrDI 5 GCAATG GCAATG(2/0)
BsrFI 172 GCCGGC [GA]^CCGG[CT]
BssHII 139 GCGCGC G^CGCGC
BstEII 210 GGTGACC G^GT[ACGT]ACC
BstPI 210 GGTGACC G^GT[ACGT]ACC
BstXI 386 CCATGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 951 CCTTAGG CC^T[ACGT]AGG
Cfr10I 172 GCCGGC [GA]^CCGG[CT]
CpoI 577 CGGACCG CG^G[AT]CCG
DpnII 411 GATC ^GATC
DrdI 931 GACCCTTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 19 GACTCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 398 AGCGCT AGC^GCT
Eco81I 951 CCTTAGG CC^T[ACGT]AGG
EcoO65I 210 GGTGACC G^GT[ACGT]ACC
Hin1I 306 GGCGCC G[GA]^CG[CT]C
HinfI 19 GACTC G^A[ACGT]TC
KasI 306 GGCGCC G^GCGCC
MaeIII 211 GTGAC ^GT[ACGT]AC
MboI 411 GATC ^GATC
MluNI 362 TGGCCA TGG^CCA
MscI 362 TGGCCA TGG^CCA
MslI 666 CATCCGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 172 GCCGGC GCC^GGC
NarI 306 GGCGCC GG^CGCC
NdeII 411 GATC ^GATC
NgoMIV 172 GCCGGC G^CCGGC
NspI 671 GCATGC [GA]CATG^[CT]
PleI 19 GACTC GAGTC(4/5)
PmaCI 202 CACGTG CAC^GTG
PmlI 202 CACGTG CAC^GTG
PshBI 916 ATTAAT AT^TAAT
PstI 589 CTGCAG CTGCA^G
PvuI 410 CGATCG CGAT^CG
PvuII 592 CAGCTG CAG^CTG
RcaI 803 TCATGA T^CATGA
RsrII 577 CGGACCG CG^G[AT]CCG
SacI 183 GAGCTC GAGCT^C
Sau3AI 411 GATC ^GATC
ScaI 539 AGTACT AGT^ACT
SexAI 553 ACCTGGT A^CC[AT]GGT
SfcI 589 CTGCAG C^T[GA][CT]AG
SfiI 721 GGCCGGGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 306 GGCGCC GGC^GCC
SphI 671 GCATGC GCATG^C
StuI 955 AGGCCT AGG^CCT
TaqI 409 TCGA T^CGA
Tsp45I 211 GTGAC ^GT[GC]AC
TthHB8I 409 TCGA T^CGA
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# TWO CUT ENZYMES ( 18 enzymes) #
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BanI 306 GGCGCC G^G[CT][GA]CC
416 GGCACC
BanII 183 GAGCTC G[GA]GC[CT]^C
737 GGGCCC
BcgI 368 CGATGCTCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
326 GCACCTACTTCG
DsaI 386 CCATGG C^C[GA][CT]GG
764 CCATGG
EagI 98 CGGCCG C^GGCCG
174 CGGCCG
EclXI 98 CGGCCG C^GGCCG
174 CGGCCG
Eco52I 98 CGGCCG C^GGCCG
174 CGGCCG
FauI 740 CCCGC CCCGC(4/6)
150 GCGGG
HgaI 42 GACGC GACGC(5/10)
440 GCGTC
MseI 917 TTAA T^TAA
1006 TTAA
NcoI 386 CCATGG C^CATGG
764 CCATGG
PflMI 201 CCACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
288 CCAGACCCTGG
SapI 378 GCTCTTC GCTCTTC(1/4)
495 GCTCTTC
SmaI 547 CCCGGG CCC^GGG
733 CCCGGG
Tru9I 917 TTAA T^TAA
1006 TTAA
Van91I 201 CCACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
288 CCAGACCCTGG
XmaCI 547 CCCGGG C^CCGGG
733 CCCGGG
XmaI 547 CCCGGG C^CCGGG
733 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
AvaI AvaII BbvI BcnI BmyI
BsaJI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DraII EaeI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI Ksp632I MaeII MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIII NlaIV PpuMI RsaI
Sau96I ScrFI SfaNI StyI TseI
Tsp509I TspRI