Restriction map for
pFN21AB6104
##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspI AvrII
BamHI BbeI BbrPI BbvCI BciVI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsiWI
BsmBI BsmI Bsp1407I BspDI BsrBI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DraI DraII DrdI DsaI
EagI EclXI Eco47III Eco52I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FseI HgaI HincII HindII
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI SspBI StyI SwaI Tth111I
XbaI XcmI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 658 GACGTC GACGT^C
AccIII 977 TCCGGA T^CCGGA
AcyI 658 GACGTC G[GA]^CG[CT]C
AhdI 1560 GACTATATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 118 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1280 CCTCAGG CC^T[ACGT]AGG
Asp700I 1632 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1560 GACTATATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1643 TGCGCA TGC^GCA
BanII 1503 GAGCTC G[GA]GC[CT]^C
BcgI 266 GCAAGGGTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 451 GCCCTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 658 GACGTC G[GA]^CG[CT]C
BsaWI 977 TCCGGA [AT]^CCGG[AT]
BseAI 977 TCCGGA T^CCGGA
BseRI 421 GAGGAG GAGGAG(10/8)
BsgI 313 GTGCAG GTGCAG(16/14)
BsiEI 1069 CGGTCG CG[GA][CT]^CG
BsmAI 1389 GTCTC GTCTC(1/5)
BspEI 977 TCCGGA T^CCGGA
BspHI 1815 TCATGA T^CATGA
BsrDI 1647 CATTGC GCAATG(2/0)
BsrFI 1307 ACCGGC [GA]^CCGG[CT]
Bsu36I 1280 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1307 ACCGGC [GA]^CCGG[CT]
DraIII 885 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1560 GACTATATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1280 CCTCAGG CC^T[ACGT]AGG
FspI 1643 TGCGCA TGC^GCA
HaeII 1112 AGCGCC [GA]GCGC^[CT]
Hin1I 658 GACGTC G[GA]^CG[CT]C
HphI 114 TCACC GGTGA(8/7)
MaeII 659 ACGT A^CGT
MroI 977 TCCGGA T^CCGGA
PaeR7I 1500 CTCGAG C^TCGAG
PvuII 1057 CAGCTG CAG^CTG
RcaI 1815 TCATGA T^CATGA
SacI 1503 GAGCTC GAGCT^C
SapI 1505 GCTCTTC GCTCTTC(1/4)
SbfI 453 CCTGCAGG CCTGCA^GG
Sse8387I 453 CCTGCAGG CCTGCA^GG
SspI 233 AATATT AAT^ATT
XhoI 1500 CTCGAG C^TCGAG
XmnI 1632 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccII 1073 CGCG CG^CG
1445 CGCG
AflIII 198 ACATGT A^C[GA][CT]GT
1409 ACATGT
AlwI 1365 GGATC GGATC(4/5)
1806 GGATC
AspHI 412 GTGCTC G[AT]GC[AT]^C
1503 GAGCTC
BalI 1533 TGGCCA TGG^CCA
1695 TGGCCA
BanI 377 GGCACC G^G[CT][GA]CC
890 GGTGCC
BbsI 1201 GAAGAC GAAGAC(2/6)
1491 GTCTTC
BclI 296 TGATCA T^GATCA
341 TGATCA
BpuAI 1201 GAAGAC GAAGAC(2/6)
1491 GTCTTC
BsiHKAI 412 GTGCTC G[AT]GC[AT]^C
1503 GAGCTC
BsmFI 1187 GGGAC GGGAC(10/14)
1119 GTCCC
BspLU11I 198 ACATGT A^CATGT
1409 ACATGT
BstUI 1073 CGCG CG^CG
1445 CGCG
BstXI 162 CCACCTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
241 CCAAAAGAATGG
BstYI 1365 GGATCT [GA]^GATC[CT]
1806 GGATCT
EaeI 1533 TGGCCA [CT]^GGCC[GA]
1695 TGGCCA
FbaI 296 TGATCA T^GATCA
341 TGATCA
HindIII 223 AAGCTT A^AGCTT
1525 AAGCTT
MflI 1365 GGATCT [GA]^GATC[CT]
1806 GGATCT
MluNI 1533 TGGCCA TGG^CCA
1695 TGGCCA
MscI 1533 TGGCCA TGG^CCA
1695 TGGCCA
MslI 304 CACTTCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1434 CATGCTCATG
MspA1I 1057 CAGCTG C[AC]G^C[GT]G
1080 CAGCGG
MvnI 1073 CGCG CG^CG
1445 CGCG
PflMI 117 CCAGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1698 CCATGAACTGG
ScaI 317 AGTACT AGT^ACT
1540 AGTACT
SmaI 1184 CCCGGG CCC^GGG
1361 CCCGGG
SmlI 1500 CTCGAG C^T[CT][GA]AG
1602 CTTGAG
StuI 425 AGGCCT AGG^CCT
1716 AGGCCT
Van91I 117 CCAGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1698 CCATGAACTGG
XhoII 1365 GGATCT [GA]^GATC[CT]
1806 GGATCT
XmaCI 1184 CCCGGG C^CCGGG
1361 CCCGGG
XmaI 1184 CCCGGG C^CCGGG
1361 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaI AvaII BbvI BcnI BmyI
BpmI BsaJI BsiYI BslI BsoBI
Bsp1286I BspMI BsrI BssKI BstAPI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII EarI EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI Ksp632I
MaeIII MboI MboII MnlI MseI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I