################################## # NO CUT ENZYMES ( 105 enzymes) # ################################## AatII Acc65I AclI AflII AflIII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BalI BamHI BbrPI BcgI BclI BfrI BlnI BlpI Bpu1102I BsaAI BsaI BseRI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BsrGI BssHII BssSI BstBI BstEII BstPI Bsu36I CelII ClaI CpoI DraI DrdI Eam1105I Eco47III Eco81I EcoO65I EcoT22I FbaI FseI HpaI KpnI MaeII MfeI MluI MluNI MscI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccI 1172 GTATAC GT^[AC][GT]AC AccIII 587 TCCGGA T^CCGGA AcyI 252 GGCGCC G[GA]^CG[CT]C AfaI 130 GTAC GT^AC AvaII 684 GGACC G^G[AT]CC BanI 252 GGCGCC G^G[CT][GA]CC BbeI 252 GGCGCC GGCGC^C BbvCI 672 CCTCAGC CCTCAGC(-5/-2) BciVI 1549 GTATCC GTATCC(6/5) BfaI 1180 CTAG C^TAG BglII 1314 AGATCT A^GATCT BsaBI 1516 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 252 GGCGCC G[GA]^CG[CT]C BsaWI 587 TCCGGA [AT]^CCGG[AT] BseAI 587 TCCGGA T^CCGGA BsgI 922 CTGCAC GTGCAG(16/14) BsmBI 348 CGTCTC CGTCTC(1/5) BspEI 587 TCCGGA T^CCGGA BspMI 1545 GCAGGT ACCTGC(4/8) BsrBI 447 GAGCGG CCGCTC(-3/-3) Bst1107I 1172 GTATAC GTA^TAC BstAPI 96 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 830 CCAACACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 1172 GTATAC GTA^TAC DraII 509 AGGCCCT [GA]G^G[ACGT]CC[CT] EagI 142 CGGCCG C^GGCCG EclXI 142 CGGCCG C^GGCCG Eco52I 142 CGGCCG C^GGCCG EcoNI 672 CCTCAGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 509 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoRV 1132 GATATC GAT^ATC Hin1I 252 GGCGCC G[GA]^CG[CT]C HincII 1565 GTCAAC GT[CT]^[GA]AC HindII 1565 GTCAAC GT[CT]^[GA]AC HindIII 1647 AAGCTT A^AGCTT KasI 252 GGCGCC G^GCGCC KspI 356 CCGCGG CCGC^GG MaeI 1180 CTAG C^TAG MamI 1516 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 587 TCCGGA T^CCGGA NarI 252 GGCGCC GG^CGCC NcoI 836 CCATGG C^CATGG PleI 971 GAGTC GAGTC(4/5) RsaI 130 GTAC GT^AC SacI 41 GAGCTC GAGCT^C SacII 356 CCGCGG CCGC^GG SfoI 252 GGCGCC GGC^GCC SmaI 65 CCCGGG CCC^GGG SphI 1575 GCATGC GCATG^C SspI 865 AATATT AAT^ATT XmaCI 65 CCCGGG C^CCGGG XmaI 65 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### Alw44I 229 GTGCAC G^TGCAC 1365 GTGCAC AlwI 1339 GATCC GGATC(4/5) 1522 GATCC AlwNI 97 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG 715 CAGCAACTG ApaLI 229 GTGCAC G^TGCAC 1365 GTGCAC AvaI 50 CCCGAG C^[CT]CG[GA]G 65 CCCGGG AviII 386 TGCGCA TGC^GCA 545 TGCGCA BanII 41 GAGCTC G[GA]GC[CT]^C 1443 GGGCTC BglI 64 GCCCGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 255 GCCCGACCGGC BpmI 491 CTGGAG CTGGAG(16/14) 904 CTGGAG BsmFI 464 GGGAC GGGAC(10/14) 487 GGGAC BsoBI 50 CCCGAG C^[CT]CG[GA]G 65 CCCGGG BsrDI 1422 CATTGC GCAATG(2/0) 1428 CATTGC DraIII 1401 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG 1598 CACTCAGTG DsaI 356 CCGCGG C^C[GA][CT]GG 836 CCATGG EaeI 142 CGGCCG [CT]^GGCC[GA] 1505 TGGCCG EcoRI 376 GAATTC G^AATTC 424 GAATTC FspI 386 TGCGCA TGC^GCA 545 TGCGCA HaeII 199 GGCGCT [GA]GCGC^[CT] 252 GGCGCC HgaI 205 GCGTC GACGC(5/10) 347 GCGTC HphI 1498 GGTGA GGTGA(8/7) 1469 TCACC MslI 289 CACTGGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1358 CATGGAGGTG NspI 1058 ACATGC [GA]CATG^[CT] 1575 GCATGC PvuII 100 CAGCTG CAG^CTG 956 CAGCTG ######################################### # 3 OR MORE CUT ENZYMES ( 78 enzymes) # ######################################### AccII AciI AcsI AluI ApoI AspHI BbsI BbvI BcnI BmyI BpuAI BsaJI BsiEI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI Ksp632I MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PstI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII