Restriction map for
pFN21AE4139
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# NO CUT ENZYMES ( 105 enzymes) #
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AatII Acc65I AclI AflII AflIII
AgeI AhdI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BalI BamHI
BbrPI BcgI BclI BfrI BlnI
BlpI Bpu1102I BsaAI BsaI BseRI
BsiWI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrGI BssHII BssSI BstBI
BstEII BstPI Bsu36I CelII ClaI
CpoI DraI DrdI Eam1105I Eco47III
Eco81I EcoO65I EcoT22I FbaI FseI
HpaI KpnI MaeII MfeI MluI
MluNI MscI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmlI SnaBI SpeI
Sse8387I SspBI StuI SwaI Tth111I
Van91I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 1172 GTATAC GT^[AC][GT]AC
AccIII 587 TCCGGA T^CCGGA
AcyI 252 GGCGCC G[GA]^CG[CT]C
AfaI 130 GTAC GT^AC
AvaII 684 GGACC G^G[AT]CC
BanI 252 GGCGCC G^G[CT][GA]CC
BbeI 252 GGCGCC GGCGC^C
BbvCI 672 CCTCAGC CCTCAGC(-5/-2)
BciVI 1549 GTATCC GTATCC(6/5)
BfaI 1180 CTAG C^TAG
BglII 1314 AGATCT A^GATCT
BsaBI 1516 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 252 GGCGCC G[GA]^CG[CT]C
BsaWI 587 TCCGGA [AT]^CCGG[AT]
BseAI 587 TCCGGA T^CCGGA
BsgI 922 CTGCAC GTGCAG(16/14)
BsmBI 348 CGTCTC CGTCTC(1/5)
BspEI 587 TCCGGA T^CCGGA
BspMI 1545 GCAGGT ACCTGC(4/8)
BsrBI 447 GAGCGG CCGCTC(-3/-3)
Bst1107I 1172 GTATAC GTA^TAC
BstAPI 96 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 830 CCAACACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1172 GTATAC GTA^TAC
DraII 509 AGGCCCT [GA]G^G[ACGT]CC[CT]
EagI 142 CGGCCG C^GGCCG
EclXI 142 CGGCCG C^GGCCG
Eco52I 142 CGGCCG C^GGCCG
EcoNI 672 CCTCAGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 509 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRV 1132 GATATC GAT^ATC
Hin1I 252 GGCGCC G[GA]^CG[CT]C
HincII 1565 GTCAAC GT[CT]^[GA]AC
HindII 1565 GTCAAC GT[CT]^[GA]AC
HindIII 1647 AAGCTT A^AGCTT
KasI 252 GGCGCC G^GCGCC
KspI 356 CCGCGG CCGC^GG
MaeI 1180 CTAG C^TAG
MamI 1516 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 587 TCCGGA T^CCGGA
NarI 252 GGCGCC GG^CGCC
NcoI 836 CCATGG C^CATGG
PleI 971 GAGTC GAGTC(4/5)
RsaI 130 GTAC GT^AC
SacI 41 GAGCTC GAGCT^C
SacII 356 CCGCGG CCGC^GG
SfoI 252 GGCGCC GGC^GCC
SmaI 65 CCCGGG CCC^GGG
SphI 1575 GCATGC GCATG^C
SspI 865 AATATT AAT^ATT
XmaCI 65 CCCGGG C^CCGGG
XmaI 65 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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Alw44I 229 GTGCAC G^TGCAC
1365 GTGCAC
AlwI 1339 GATCC GGATC(4/5)
1522 GATCC
AlwNI 97 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
715 CAGCAACTG
ApaLI 229 GTGCAC G^TGCAC
1365 GTGCAC
AvaI 50 CCCGAG C^[CT]CG[GA]G
65 CCCGGG
AviII 386 TGCGCA TGC^GCA
545 TGCGCA
BanII 41 GAGCTC G[GA]GC[CT]^C
1443 GGGCTC
BglI 64 GCCCGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
255 GCCCGACCGGC
BpmI 491 CTGGAG CTGGAG(16/14)
904 CTGGAG
BsmFI 464 GGGAC GGGAC(10/14)
487 GGGAC
BsoBI 50 CCCGAG C^[CT]CG[GA]G
65 CCCGGG
BsrDI 1422 CATTGC GCAATG(2/0)
1428 CATTGC
DraIII 1401 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
1598 CACTCAGTG
DsaI 356 CCGCGG C^C[GA][CT]GG
836 CCATGG
EaeI 142 CGGCCG [CT]^GGCC[GA]
1505 TGGCCG
EcoRI 376 GAATTC G^AATTC
424 GAATTC
FspI 386 TGCGCA TGC^GCA
545 TGCGCA
HaeII 199 GGCGCT [GA]GCGC^[CT]
252 GGCGCC
HgaI 205 GCGTC GACGC(5/10)
347 GCGTC
HphI 1498 GGTGA GGTGA(8/7)
1469 TCACC
MslI 289 CACTGGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1358 CATGGAGGTG
NspI 1058 ACATGC [GA]CATG^[CT]
1575 GCATGC
PvuII 100 CAGCTG CAG^CTG
956 CAGCTG
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# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AcsI AluI ApoI
AspHI BbsI BbvI BcnI BmyI
BpuAI BsaJI BsiEI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII EarI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI Ksp632I
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PstI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII