Restriction map for
pFN21AE5637
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI AocI
Aor51HI AscI Asp718I AspEI AspI
AvaI AvaII AviII BbeI BbrPI
BbvCI BciVI BcnI BfrI BglI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
EcoT22I FauI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HpaI HpaII HphI KasI KpnI
KspI MamI MfeI MluI MroI
MspA1I MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI Sse8387I SspBI StuI
SwaI TaqI Tth111I TthHB8I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 259 GTAGAC GT^[AC][GT]AC
Alw44I 773 GTGCAC G^TGCAC
AlwNI 785 CAGTCGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1026 GGGCCC GGGCC^C
ApaLI 773 GTGCAC G^TGCAC
AseI 1122 ATTAAT AT^TAAT
AsnI 1122 ATTAAT AT^TAAT
Asp700I 610 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 773 GTGCAC G[AT]GC[AT]^C
AvrII 1402 CCTAGG C^CTAGG
BanI 1174 GGTGCC G^G[CT][GA]CC
BanII 1026 GGGCCC G[GA]GC[CT]^C
BcgI 1219 GCAAGAGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1149 TGATCA T^GATCA
BglII 1040 AGATCT A^GATCT
BlnI 1402 CCTAGG C^CTAGG
BpmI 266 CTGGAG CTGGAG(16/14)
BsiHKAI 773 GTGCAC G[AT]GC[AT]^C
BsmAI 989 GAGAC GTCTC(1/5)
BsmFI 1066 GGGAC GGGAC(10/14)
BspHI 1016 TCATGA T^CATGA
BsrI 1353 CCAGT ACTGG(1/-1)
BstXI 1388 CCAAAATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 1026 GGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 776 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 1026 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 467 GAATTC G^AATTC
FbaI 1149 TGATCA T^GATCA
HincII 919 GTCAAC GT[CT]^[GA]AC
HindII 919 GTCAAC GT[CT]^[GA]AC
HindIII 253 AAGCTT A^AGCTT
MaeII 982 ACGT A^CGT
NheI 360 GCTAGC G^CTAGC
PshAI 1436 GACTTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1122 ATTAAT AT^TAAT
RcaI 1016 TCATGA T^CATGA
SpeI 141 ACTAGT A^CTAGT
SphI 228 GCATGC GCATG^C
SspI 133 AATATT AAT^ATT
TspRI 780 CAGTG CA[GC]TG[ACGT][ACGT]^
XmnI 610 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AciI 110 CCGC CCGC(-3/-1)
579 CCGC
BalI 753 TGGCCA TGG^CCA
1385 TGGCCA
BamHI 498 GGATCC G^GATCC
1398 GGATCC
BbsI 390 GAAGAC GAAGAC(2/6)
592 GAAGAC
BbvI 790 GCTGC GCAGC(8/12)
1471 GCTGC
BmyI 773 GTGCAC G[AGT]GC[ACT]^C
1026 GGGCCC
BpuAI 390 GAAGAC GAAGAC(2/6)
592 GAAGAC
BsgI 407 GTGCAG GTGCAG(16/14)
782 GTGCAG
BsmI 84 GAATGC GAATGC(1/-1)
450 GAATGC
Bsp1286I 773 GTGCAC G[AGT]GC[ACT]^C
1026 GGGCCC
BsrDI 510 CATTGC GCAATG(2/0)
53 GCAATG
BstAPI 775 GCACTCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
784 GCAGTCGCTGC
Cfr13I 1026 GGGCC G^G[ACGT]CC
1027 GGCCC
EarI 73 GAAGAG CTCTTC(1/4)
187 GAAGAG
EcoT14I 15 CCTTGG C^C[AT][AT]GG
1402 CCTAGG
Ksp632I 73 GAAGAG CTCTTC(1/4)
187 GAAGAG
MaeIII 739 GTGAC ^GT[ACGT]AC
1356 GTCAC
MluNI 753 TGGCCA TGG^CCA
1385 TGGCCA
MscI 753 TGGCCA TGG^CCA
1385 TGGCCA
PflMI 472 CCATTTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1377 CCAACAAATGG
PleI 595 GACTC GAGTC(4/5)
1099 GAGTC
Sau96I 1026 GGGCC G^G[ACGT]CC
1027 GGCCC
SfaNI 664 GATGC GCATC(5/9)
196 GCATC
StyI 15 CCTTGG C^C[AT][AT]GG
1402 CCTAGG
TseI 790 GCTGC G^C[AT]GC
1471 GCTGC
Tsp45I 739 GTGAC ^GT[GC]AC
1356 GTCAC
Van91I 472 CCATTTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1377 CCAACAAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AcsI AfaI AflIII AluI AlwI
ApoI BfaI BsaJI BsiYI BslI
BspLU11I BssKI BstF5I BstNI BstYI
Cac8I DdeI DpnII DraI EaeI
EcoRII Fnu4HI FokI HaeIII HinfI
ItaI MaeI MboI MboII MflI
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV NspI RsaI
Sau3AI ScrFI TfiI Tru9I Tsp509I
XhoII