##################################
# NO CUT ENZYMES (  212 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        AluI        Alw44I      AlwI        AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BamHI       BanI        BbeI        
BbrPI       BbvCI       BbvI        BcgI        BclI        
BcnI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmAI       
BsmBI       BsmFI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DdeI        DpnII       DraI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FbaI        
Fnu4HI      FseI        FspI        HapII       HgaI        
Hin1I       HincII      HindII      HindIII     HinfI       
HpaI        HpaII       HphI        ItaI        KasI        
KpnI        Ksp632I     KspI        MaeI        MamI        
MboI        MfeI        MflI        MluI        MluNI       
MnlI        MroI        MscI        MseI        MslI        
MspA1I      MspI        MunI        NaeI        NarI        
NciI        NcoI        NdeI        NdeII       NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        Sau3AI      SbfI        ScaI        
SexAI       SfaNI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TaqI        TfiI        
Tru9I       TseI        TspRI       Tth111I     TthHB8I     
Van91I      XbaI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   25 enzymes) #
###################################

AccII          20  CGCG                 CG^CG
AlwNI          55  CAGTACCTG            CAG[ACGT][ACGT][ACGT]^CTG
BanII          88  GAGCCC               G[GA]GC[CT]^C
BbsI          160  GTCTTC               GAAGAC(2/6)
BciVI         154  GGATAC               GTATCC(6/5)
BmyI           88  GAGCCC               G[AGT]GC[ACT]^C
BpmI           85  CTGGAG               CTGGAG(16/14)
BpuAI         160  GTCTTC               GAAGAC(2/6)
Bsp1286I       88  GAGCCC               G[AGT]GC[ACT]^C
BspMI          59  ACCTGC               ACCTGC(4/8)
BsrBI          97  GAGCGG               CCGCTC(-3/-3)
BsrI           54  CCAGT                ACTGG(1/-1)
BstUI          20  CGCG                 CG^CG
Cfr13I         23  GGGCC                G^G[ACGT]CC
HaeIII         24  GGCC                 GG^CC
MaeII         158  ACGT                 A^CGT
MaeIII         67  GTCAC                ^GT[ACGT]AC
MboII         161  TCTTC                GAAGA(8/7)
MvnI           20  CGCG                 CG^CG
NlaIV          87  GGAGCC               GG[ACGT]^[ACGT]CC
NspI           80  ACATGC               [GA]CATG^[CT]
Sau96I         23  GGGCC                G^G[ACGT]CC
Tsp45I         67  GTCAC                ^GT[GC]AC
Tsp509I       112  AATT                 ^AATT
XcmI          128  CCATTGTGGGGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AfaI           57  GTAC                 GT^AC
              208  GTAC                
BsaJI          91  CCCTGG               C^C[ACGT][ACGT]GG
               92  CCTGGG              
BsiYI          92  CCTGGGAGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              128  CCATTGTGGGG         
BslI           92  CCTGGGAGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              128  CCATTGTGGGG         
BstF5I          8  GGATG                GGATG(2/0)
              137  GGATG               
Cac8I          15  GCTGGC               GC[ACGT]^[ACGT]GC
               21  GCGGGC              
FauI            4  GCGGG                CCCGC(4/6)
               21  GCGGG               
FokI            8  GGATG                GGATG(9/13)
              137  GGATG               
HaeII          12  GGCGCT               [GA]GCGC^[CT]
               72  GGCGCT              
RsaI           57  GTAC                 GT^AC
              208  GTAC                


#########################################
# 3 OR MORE CUT ENZYMES (   11 enzymes) #
#########################################

AciI        BssKI       BstNI       CfoI        EcoRII      
HhaI        HinP1I      MvaI        MwoI        NlaIII      
ScrFI