################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BamHI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaI BsaWI BseAI BsiHKAI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII Eam1105I EarI Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HindIII HpaI KpnI Ksp632I KspI MfeI MluI MroI MunI NcoI NdeI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfuI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tsp45I Tsp509I TspRI Tth111I Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AflIII 788 ACGTGT A^C[GA][CT]GT ApaI 291 GGGCCC GGGCC^C Asp700I 636 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 575 TGGCCA TGG^CCA BbsI 1443 GTCTTC GAAGAC(2/6) BfaI 1150 CTAG C^TAG BpuAI 1443 GTCTTC GAAGAC(2/6) BsaBI 573 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 946 CTGCAC GTGCAG(16/14) BsiWI 1118 CGTACG C^GTACG BspMI 1223 ACCTGC ACCTGC(4/8) BsrBI 825 CCGCTC CCGCTC(-3/-3) BssHII 868 GCGCGC G^CGCGC BstAPI 300 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 1112 AGATCC [GA]^GATC[CT] MaeI 1150 CTAG C^TAG MamI 573 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 1112 AGATCC [GA]^GATC[CT] MluNI 575 TGGCCA TGG^CCA MscI 575 TGGCCA TGG^CCA MspA1I 301 CAGCTG C[AC]G^C[GT]G NheI 1149 GCTAGC G^CTAGC PpuMI 1192 GGGACCT [GA]G^G[AT]CC[CT] PvuII 301 CAGCTG CAG^CTG SfcI 1020 CTACAG C^T[GA][CT]AG SfiI 843 GGCCAAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 444 CGCCGGCG C[GA]^CCGG[CT]G SmaI 744 CCCGGG CCC^GGG XhoII 1112 AGATCC [GA]^GATC[CT] XmaCI 744 CCCGGG C^CCGGG XmaI 744 CCCGGG C^CCGGG XmnI 636 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AfaI 534 GTAC GT^AC 1119 GTAC AlwI 36 GATCC GGATC(4/5) 1113 GATCC AlwNI 298 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG 301 CAGCTGCTG BanII 291 GGGCCC G[GA]GC[CT]^C 588 GAGCCC BglI 414 GCCCGTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 844 GCCAAGCTGGC BseRI 1320 CTCCTC GAGGAG(10/8) 460 GAGGAG BspHI 569 TCATGA T^CATGA 632 TCATGA DdeI 1037 CTCAG C^T[ACGT]AG 1084 CTGAG DpnII 36 GATC ^GATC 1113 GATC DrdI 1093 GACCGGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1392 GACGGGACTGTC DsaI 108 CCACGG C^C[GA][CT]GG 806 CCACGG HgaI 258 GACGC GACGC(5/10) 1315 GCGTC HinfI 1035 GACTC G^A[ACGT]TC 1360 GAGTC MaeIII 977 GTTAC ^GT[ACGT]AC 1244 GTTAC MboI 36 GATC ^GATC 1113 GATC MseI 1366 TTAA T^TAA 1459 TTAA NaeI 445 GCCGGC GCC^GGC 907 GCCGGC NdeII 36 GATC ^GATC 1113 GATC NgoMIV 445 GCCGGC G^CCGGC 907 GCCGGC PleI 1035 GACTC GAGTC(4/5) 1360 GAGTC RcaI 569 TCATGA T^CATGA 632 TCATGA RsaI 534 GTAC GT^AC 1119 GTAC Sau3AI 36 GATC ^GATC 1113 GATC TaqI 778 TCGA T^CGA 1345 TCGA Tru9I 1366 TTAA T^TAA 1459 TTAA TthHB8I 778 TCGA T^CGA 1345 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AccII AciI AcyI AluI AvaI AvaII BanI BbeI BbvI BcnI BmyI BsaHI BsaJI BsiEI BsiYI BslI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DraII EaeI EagI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HincII HindII HpaII HphI ItaI KasI MaeII MboII MnlI MslI MspI MvaI MvnI MwoI NarI NciI NlaIII NlaIV Sau96I ScrFI SfaNI SfoI TseI