Restriction map for
pFN21AE1745
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BanI BbeI BbrPI
BbvCI BcgI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BsrI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI ClaI CpoI DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoT22I FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HpaI
KasI KpnI KspI MaeI MaeII
MamI MfeI MluI MluNI MroI
MscI MslI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacI SacII SalI SapI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspBI SspI SwaI TaqI TspRI
Tth111I TthHB8I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AciI 96 CCGC CCGC(-3/-1)
AcsI 145 AAATTT [GA]^AATT[CT]
AlwNI 724 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 612 GGGCCC GGGCC^C
ApoI 145 AAATTT [GA]^AATT[CT]
AspHI 39 GTGCTC G[AT]GC[AT]^C
AvaII 136 GGTCC G^G[AT]CC
BamHI 510 GGATCC G^GATCC
BciVI 649 GTATCC GTATCC(6/5)
BpmI 42 CTCCAG CTGGAG(16/14)
BsgI 200 GTGCAG GTGCAG(16/14)
BsiEI 661 CGGTCG CG[GA][CT]^CG
BsiHKAI 39 GTGCTC G[AT]GC[AT]^C
BsmI 79 GAATGC GAATGC(1/-1)
BsrFI 707 ACCGGC [GA]^CCGG[CT]
BstAPI 74 GCACAGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 707 ACCGGC [GA]^CCGG[CT]
EcoRV 328 GATATC GAT^ATC
EcoT14I 225 CCTTGG C^C[AT][AT]GG
FauI 95 CCCGC CCCGC(4/6)
HindIII 268 AAGCTT A^AGCTT
MseI 596 TTAA T^TAA
MspA1I 556 CAGCTG C[AC]G^C[GT]G
PvuII 556 CAGCTG CAG^CTG
SbfI 729 CCTGCAGG CCTGCA^GG
SmlI 271 CTTGAG C^T[CT][GA]AG
Sse8387I 729 CCTGCAGG CCTGCA^GG
StuI 518 AGGCCT AGG^CCT
StyI 225 CCTTGG C^C[AT][AT]GG
TfiI 695 GAATC G^A[AT]TC
Tru9I 596 TTAA T^TAA
Tsp45I 220 GTCAC ^GT[GC]AC
XcmI 359 CCAGCCTGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
BcnI 432 CCGGG CC^[GC]GG
469 CCGGG
BstF5I 295 CATCC GGATG(2/0)
666 GGATG
BstYI 510 GGATCC [GA]^GATC[CT]
725 AGATCC
DdeI 474 CTCAG C^T[ACGT]AG
499 CTGAG
DraII 611 GGGGCCC [GA]G^G[ACGT]CC[CT]
612 GGGCCCT
EarI 317 CTCTTC CTCTTC(1/4)
617 CTCTTC
EcoO109I 611 GGGGCCC [GA]G^G[ACGT]CC[CT]
612 GGGCCCT
FokI 295 CATCC GGATG(9/13)
666 GGATG
Ksp632I 317 CTCTTC CTCTTC(1/4)
617 CTCTTC
MflI 510 GGATCC [GA]^GATC[CT]
725 AGATCC
NciI 432 CCGGG CC^[GC]GG
469 CCGGG
PleI 503 GACTC GAGTC(4/5)
604 GACTC
PstI 522 CTGCAG CTGCA^G
730 CTGCAG
SfaNI 667 GATGC GCATC(5/9)
644 GCATC
SfcI 522 CTGCAG C^T[GA][CT]AG
730 CTGCAG
XhoII 510 GGATCC [GA]^GATC[CT]
725 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AluI AlwI BanII BbsI BbvI
BmyI BpuAI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BssKI BstNI
Cac8I Cfr13I DpnII EcoRII Fnu4HI
HaeIII HapII HinfI HpaII HphI
ItaI MaeIII MboI MboII MnlI
MspI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI TseI
Tsp509I