Restriction map for
pFN21AE1872
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AflIII AgeI AhdI Aor51HI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BsaAI BsaBI BsaHI BsaI BsiEI
BsiWI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstYI BstZ17I Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeII
MamI MfeI MflI MluI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SwaI TaqI Tsp45I TthHB8I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 685 CGCG CG^CG
Alw44I 257 GTGCAC G^TGCAC
AlwI 609 GGATC GGATC(4/5)
AocI 740 CCTCAGG CC^T[ACGT]AGG
ApaI 1017 GGGCCC GGGCC^C
ApaLI 257 GTGCAC G^TGCAC
AspI 991 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 854 CCCGAG C^[CT]CG[GA]G
BbsI 240 GAAGAC GAAGAC(2/6)
BfaI 838 CTAG C^TAG
BpuAI 240 GAAGAC GAAGAC(2/6)
BsmBI 357 CGTCTC CGTCTC(1/5)
BsmI 900 GAATGC GAATGC(1/-1)
BsoBI 854 CCCGAG C^[CT]CG[GA]G
BspMI 765 GCAGGT ACCTGC(4/8)
BstUI 685 CGCG CG^CG
BstXI 229 CCAGCTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 740 CCTCAGG CC^T[ACGT]AGG
DpnII 610 GATC ^GATC
DsaI 1065 CCATGG C^C[GA][CT]GG
Eco81I 740 CCTCAGG CC^T[ACGT]AGG
EcoT14I 1065 CCATGG C^C[AT][AT]GG
FauI 930 CCCGC CCCGC(4/6)
HphI 665 TCACC GGTGA(8/7)
MaeI 838 CTAG C^TAG
MboI 610 GATC ^GATC
MslI 834 CATCCTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 230 CAGCTG C[AC]G^C[GT]G
MvnI 685 CGCG CG^CG
NcoI 1065 CCATGG C^CATGG
NdeII 610 GATC ^GATC
NspI 129 ACATGC [GA]CATG^[CT]
PflFI 991 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 890 CCATCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 616 AGGTCCC [GA]G^G[AT]CC[CT]
PstI 984 CTGCAG CTGCA^G
PvuII 230 CAGCTG CAG^CTG
SacI 961 GAGCTC GAGCT^C
SapI 963 GCTCTTC GCTCTTC(1/4)
Sau3AI 610 GATC ^GATC
SfcI 984 CTGCAG C^T[GA][CT]AG
SmlI 823 CTTGAG C^T[CT][GA]AG
SspI 189 AATATT AAT^ATT
StyI 1065 CCATGG C^C[AT][AT]GG
Tth111I 991 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 890 CCATCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccIII 35 TCCGGA T^CCGGA
113 TCCGGA
AcsI 277 GAATTT [GA]^AATT[CT]
1047 GAATTT
AfaI 385 GTAC GT^AC
1002 GTAC
ApoI 277 GAATTT [GA]^AATT[CT]
1047 GAATTT
BalI 363 TGGCCA TGG^CCA
842 TGGCCA
BcnI 76 CCCGG CC^[GC]GG
150 CCGGG
BsaWI 35 TCCGGA [AT]^CCGG[AT]
113 TCCGGA
BseAI 35 TCCGGA T^CCGGA
113 TCCGGA
BseRI 496 GAGGAG GAGGAG(10/8)
499 GAGGAG
BsmAI 989 GAGAC GTCTC(1/5)
358 GTCTC
BspEI 35 TCCGGA T^CCGGA
113 TCCGGA
EaeI 363 TGGCCA [CT]^GGCC[GA]
842 TGGCCA
EcoNI 416 CCTCTGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
919 CCTGGGCCAGG
HaeII 806 AGCGCC [GA]GCGC^[CT]
872 AGCGCC
MaeIII 577 GTTAC ^GT[ACGT]AC
1039 GTAAC
MluNI 363 TGGCCA TGG^CCA
842 TGGCCA
MroI 35 TCCGGA T^CCGGA
113 TCCGGA
MscI 363 TGGCCA TGG^CCA
842 TGGCCA
MseI 194 TTAA T^TAA
281 TTAA
NciI 76 CCCGG CC^[GC]GG
150 CCGGG
RsaI 385 GTAC GT^AC
1002 GTAC
Tru9I 194 TTAA T^TAA
281 TTAA
Tsp509I 278 AATT ^AATT
1048 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AluI AlwNI AspHI AvaII
BanII BbvI BlpI BmyI BpmI
Bpu1102I BsaJI BsgI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstAPI BstF5I BstNI Cac8I CelII
CfoI Cfr13I DdeI DraII EarI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI Ksp632I MboII MnlI MspI
MvaI MwoI NlaIII NlaIV PleI
Sau96I ScrFI SfaNI StuI TfiI
TseI TspRI XcmI