Restriction map for
pFN21AE3031
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BamHI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BseAI
BsgI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I CelII ClaI CpoI DraI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco52I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HgaI Hin1I
HincII HindII HindIII HinfI HpaI
KasI KpnI MaeI MaeII MamI
MfeI MluI MluNI MroI MscI
MslI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SalI
SapI SbfI SexAI SfcI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI TfiI Tth111I Van91I XbaI
XcmI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AfaI 240 GTAC GT^AC
AocI 34 CCTGAGG CC^T[ACGT]AGG
Aor51HI 248 AGCGCT AGC^GCT
BanII 55 GAGCTC G[GA]GC[CT]^C
BglI 172 GCCTACATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 419 AGATCT A^GATCT
BsaI 321 GGTCTC GGTCTC(1/5)
BsaWI 425 ACCGGA [AT]^CCGG[AT]
BseRI 37 GAGGAG GAGGAG(10/8)
BsiEI 255 CGACCG CG[GA][CT]^CG
BsmAI 322 GTCTC GTCTC(1/5)
BsrDI 360 CATTGC GCAATG(2/0)
BsrFI 26 ACCGGC [GA]^CCGG[CT]
BstYI 419 AGATCT [GA]^GATC[CT]
Bsu36I 34 CCTGAGG CC^T[ACGT]AGG
Cfr10I 26 ACCGGC [GA]^CCGG[CT]
DsaI 182 CCGCGG C^C[GA][CT]GG
EaeI 179 TGGCCG [CT]^GGCC[GA]
EarI 220 CTCTTC CTCTTC(1/4)
Eco47III 248 AGCGCT AGC^GCT
Eco81I 34 CCTGAGG CC^T[ACGT]AGG
HphI 89 TCACC GGTGA(8/7)
Ksp632I 220 CTCTTC CTCTTC(1/4)
KspI 182 CCGCGG CCGC^GG
MaeIII 337 GTGAC ^GT[ACGT]AC
MflI 419 AGATCT [GA]^GATC[CT]
MseI 263 TTAA T^TAA
NlaIII 177 CATG CATG^
PaeR7I 11 CTCGAG C^TCGAG
PvuII 332 CAGCTG CAG^CTG
RsaI 240 GTAC GT^AC
SacI 55 GAGCTC GAGCT^C
SacII 182 CCGCGG CCGC^GG
ScaI 239 AGTACT AGT^ACT
SmlI 11 CTCGAG C^T[CT][GA]AG
StuI 80 AGGCCT AGG^CCT
Tru9I 263 TTAA T^TAA
Tsp45I 337 GTGAC ^GT[GC]AC
Tsp509I 261 AATT ^AATT
XhoI 11 CTCGAG C^TCGAG
XhoII 419 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 69 CGCG CG^CG
183 CGCG
AspHI 8 GTGCTC G[AT]GC[AT]^C
55 GAGCTC
AvaII 24 GGACC G^G[AT]CC
459 GGACC
BanI 29 GGCACC G^G[CT][GA]CC
438 GGCACC
BmyI 8 GTGCTC G[AGT]GC[ACT]^C
55 GAGCTC
BpmI 58 CTCCAG CTGGAG(16/14)
193 CTGGAG
BsiHKAI 8 GTGCTC G[AT]GC[AT]^C
55 GAGCTC
Bsp1286I 8 GTGCTC G[AGT]GC[ACT]^C
55 GAGCTC
BstF5I 105 CATCC GGATG(2/0)
384 CATCC
BstUI 69 CGCG CG^CG
183 CGCG
Cac8I 123 GCTGGC GC[ACGT]^[ACGT]GC
448 GCCAGC
DpnII 16 GATC ^GATC
420 GATC
FokI 105 CATCC GGATG(9/13)
384 CATCC
HaeII 186 GGCGCT [GA]GCGC^[CT]
248 AGCGCT
MboI 16 GATC ^GATC
420 GATC
MspA1I 182 CCGCGG C[AC]G^C[GT]G
332 CAGCTG
MvnI 69 CGCG CG^CG
183 CGCG
NdeII 16 GATC ^GATC
420 GATC
Sau3AI 16 GATC ^GATC
420 GATC
SmaI 344 CCCGGG CCC^GGG
442 CCCGGG
TaqI 12 TCGA T^CGA
254 TCGA
TspRI 6 CAGTG CA[GC]TG[ACGT][ACGT]^
297 CAGTG
TthHB8I 12 TCGA T^CGA
254 TCGA
XmaCI 344 CCCGGG C^CCGGG
442 CCCGGG
XmaI 344 CCCGGG C^CCGGG
442 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AluI AvaI BbvI BcnI
BsaJI BsiYI BslI BsmFI BsoBI
BsrI BssKI BstNI CfoI Cfr13I
DdeI EcoRII Fnu4HI HaeIII HapII
HhaI HinP1I HpaII ItaI MboII
MnlI MspI MvaI MwoI NciI
NlaIV Sau96I ScrFI SfaNI TseI