Restriction map for
pFN21AE4133
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# NO CUT ENZYMES ( 133 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Aor51HI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BalI BamHI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstUI BstZ17I CelII Cfr10I ClaI
CpoI DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoRI
EcoT22I FbaI FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PstI PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SwaI Tth111I
XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Alw44I 555 GTGCAC G^TGCAC
ApaI 1343 GGGCCC GGGCC^C
ApaLI 555 GTGCAC G^TGCAC
Asp700I 1578 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 391 CCCGGG C^[CT]CG[GA]G
BanI 1396 GGCACC G^G[CT][GA]CC
BfaI 838 CTAG C^TAG
BsaAI 610 TACGTG [CT]AC^GT[GA]
BsmBI 274 CGTCTC CGTCTC(1/5)
BsmI 1521 GAATGC GAATGC(1/-1)
BsoBI 391 CCCGGG C^[CT]CG[GA]G
BspHI 1220 TCATGA T^CATGA
BstAPI 177 GCAGCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1178 TTCGAA TT^CGAA
BstYI 903 GGATCT [GA]^GATC[CT]
DraI 1144 TTTAAA TTT^AAA
DraIII 928 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 419 CCGTGG C^C[GA][CT]GG
EcoRV 1190 GATATC GAT^ATC
EcoT14I 791 CCTTGG C^C[AT][AT]GG
HaeII 1349 AGCGCC [GA]GCGC^[CT]
MaeI 838 CTAG C^TAG
MflI 903 GGATCT [GA]^GATC[CT]
NspV 1178 TTCGAA TT^CGAA
PflMI 718 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 799 GAGTC GAGTC(4/5)
PshAI 1243 GACCAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 481 CAGCTG CAG^CTG
RcaI 1220 TCATGA T^CATGA
SfuI 1178 TTCGAA TT^CGAA
SmaI 391 CCCGGG CCC^GGG
SspI 997 AATATT AAT^ATT
StuI 1149 AGGCCT AGG^CCT
StyI 791 CCTTGG C^C[AT][AT]GG
Van91I 718 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 903 GGATCT [GA]^GATC[CT]
XmaCI 391 CCCGGG C^CCGGG
XmaI 391 CCCGGG C^CCGGG
XmnI 1578 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwI 1211 GATCC GGATC(4/5)
903 GGATC
BglI 400 GCCTTTAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1087 GCCTGTGTGGC
BpmI 277 CTCCAG CTGGAG(16/14)
888 CTCCAG
BsgI 454 GTGCAG GTGCAG(16/14)
626 GTGCAG
BspMI 1561 ACCTGC ACCTGC(4/8)
456 GCAGGT
BstEII 292 GGTGACC G^GT[ACGT]ACC
1044 GGTGACC
BstPI 292 GGTGACC G^GT[ACGT]ACC
1044 GGTGACC
BstXI 677 CCACACAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1309 CCAGGTGTGTGG
DpnII 904 GATC ^GATC
1211 GATC
DraII 501 GGGGCCT [GA]G^G[ACGT]CC[CT]
804 AGGCCCT
EcoO109I 501 GGGGCCT [GA]G^G[ACGT]CC[CT]
804 AGGCCCT
EcoO65I 292 GGTGACC G^GT[ACGT]ACC
1044 GGTGACC
MboI 904 GATC ^GATC
1211 GATC
MslI 135 CATCACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1372 CACCTGGATG
NdeII 904 GATC ^GATC
1211 GATC
SacI 168 GAGCTC GAGCT^C
1360 GAGCTC
Sau3AI 904 GATC ^GATC
1211 GATC
SmlI 379 CTCAAG C^T[CT][GA]AG
1030 CTCAAG
TaqI 206 TCGA T^CGA
1179 TCGA
TfiI 114 GAATC G^A[AT]TC
962 GATTC
Tsp509I 1060 AATT ^AATT
1486 AATT
TthHB8I 206 TCGA T^CGA
1179 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AfaI AluI AlwNI AocI
AspHI AvaII BanII BbvI BcnI
BmyI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Bsu36I Cac8I
CfoI Cfr13I DdeI EarI Eco81I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboII MnlI MseI MspA1I MspI
MvaI MwoI NciI NlaIII NlaIV
NspI RsaI Sau96I ScrFI SfaNI
Tru9I TseI Tsp45I TspRI