Restriction map for
pFN21AB0433
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflIII AgeI AhdI Alw44I
AocI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BbeI BbsI BbvCI BcgI
BciVI BclI BlnI BlpI Bpu1102I
BpuAI BsaHI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI Hin1I HindIII
HpaI KasI KpnI KspI MfeI
MluI MluNI MroI MscI MunI
MvnI NarI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SpeI Sse8387I
SspBI SspI SwaI Tth111I Van91I
XbaI XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 20 GTCTAC GT^[AC][GT]AC
AcsI 346 AAATTC [GA]^AATT[CT]
Aor51HI 232 AGCGCT AGC^GCT
ApaI 238 GGGCCC GGGCC^C
ApoI 346 AAATTC [GA]^AATT[CT]
Asp700I 342 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1240 GTGCTC G[AT]GC[AT]^C
BamHI 1353 GGATCC G^GATCC
BbrPI 178 CACGTG CAC^GTG
BfaI 329 CTAG C^TAG
BglI 1253 GCCGGCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1302 AGATCT A^GATCT
BsaBI 1267 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1130 GGTCTC GGTCTC(1/5)
BsaWI 121 TCCGGT [AT]^CCGG[AT]
BsgI 769 GTGCAG GTGCAG(16/14)
BsiHKAI 1240 GTGCTC G[AT]GC[AT]^C
BsmI 517 GCATTC GAATGC(1/-1)
BsrFI 1253 GCCGGC [GA]^CCGG[CT]
BstAPI 1233 GCATGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 1253 GCCGGC [GA]^CCGG[CT]
EarI 135 GAAGAG CTCTTC(1/4)
Eco47III 232 AGCGCT AGC^GCT
FauI 1250 CCCGC CCCGC(4/6)
HincII 1023 GTTGAC GT[CT]^[GA]AC
HindII 1023 GTTGAC GT[CT]^[GA]AC
Ksp632I 135 GAAGAG CTCTTC(1/4)
MaeI 329 CTAG C^TAG
MamI 1267 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 11 CACTGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1253 GCCGGC GCC^GGC
NgoMIV 1253 GCCGGC G^CCGGC
PmaCI 178 CACGTG CAC^GTG
PmlI 178 CACGTG CAC^GTG
PstI 130 CTGCAG CTGCA^G
SexAI 975 ACCTGGT A^CC[AT]GGT
SmaI 71 CCCGGG CCC^GGG
SnaBI 413 TACGTA TAC^GTA
SphI 1233 GCATGC GCATG^C
StuI 139 AGGCCT AGG^CCT
Tsp509I 347 AATT ^AATT
XcmI 665 CCATGTTGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 71 CCCGGG C^CCGGG
XmaI 71 CCCGGG C^CCGGG
XmnI 342 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflII 801 CTTAAG C^TTAAG
940 CTTAAG
AvaI 71 CCCGGG C^[CT]CG[GA]G
1124 CTCGGG
AvaII 936 GGTCC G^G[AT]CC
966 GGACC
BanI 41 GGTGCC G^G[CT][GA]CC
208 GGTGCC
BanII 238 GGGCCC G[GA]GC[CT]^C
533 GGGCTC
BfrI 801 CTTAAG C^TTAAG
940 CTTAAG
BsmAI 508 GTCTC GTCTC(1/5)
1131 GTCTC
BsoBI 71 CCCGGG C^[CT]CG[GA]G
1124 CTCGGG
BstXI 150 CCAGCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
926 CCAGGGATCTGG
CfoI 76 GCGC GCG^C
233 GCGC
EcoT14I 51 CCTTGG C^C[AT][AT]GG
764 CCAAGG
HaeII 75 GGCGCT [GA]GCGC^[CT]
232 AGCGCT
HhaI 76 GCGC GCG^C
233 GCGC
HinP1I 76 GCGC G^CGC
233 GCGC
HinfI 252 GAATC G^A[ACGT]TC
706 GAATC
MspA1I 659 CAGCTG C[AC]G^C[GT]G
1074 CAGCTG
NspI 1226 ACATGC [GA]CATG^[CT]
1233 GCATGC
PvuII 659 CAGCTG CAG^CTG
1074 CAGCTG
StyI 51 CCTTGG C^C[AT][AT]GG
764 CCAAGG
TaqI 537 TCGA T^CGA
972 TCGA
TfiI 252 GAATC G^A[AT]TC
706 GAATC
TthHB8I 537 TCGA T^CGA
972 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
BbvI BcnI BmyI BpmI BsaAI
BsaJI BseRI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HapII
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI Tru9I
TseI Tsp45I TspRI XhoII