Restriction map for
pFN21AE5159
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# NO CUT ENZYMES ( 179 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
AluI AlwNI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BalI BamHI BbeI BbrPI
BbsI BbvCI BclI BfaI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI
BstUI BstXI Bsu36I CelII CfoI
Cfr10I Cfr13I CpoI DraI DraII
DraIII DrdI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HaeII
HaeIII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MaeI MaeII
MamI MluI MluNI MroI MscI
MspA1I MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI Sau96I SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI StuI StyI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 257 GTATAC GT^[AC][GT]AC
AciI 162 GCGG CCGC(-3/-1)
AcsI 26 AAATTT [GA]^AATT[CT]
AfaI 215 GTAC GT^AC
Alw44I 347 GTGCAC G^TGCAC
AlwI 424 GATCC GGATC(4/5)
ApaLI 347 GTGCAC G^TGCAC
ApoI 26 AAATTT [GA]^AATT[CT]
AspHI 347 GTGCAC G[AT]GC[AT]^C
BanI 312 GGTGCC G^G[CT][GA]CC
BbvI 263 GCTGC GCAGC(8/12)
BcgI 181 CGATGGCGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 309 CCGGG CC^[GC]GG
BsiHKAI 347 GTGCAC G[AT]GC[AT]^C
BspDI 179 ATCGAT AT^CGAT
BspMI 368 GCAGGT ACCTGC(4/8)
BsrI 457 CCAGT ACTGG(1/-1)
Bst1107I 257 GTATAC GTA^TAC
BstEII 247 GGTAACC G^GT[ACGT]ACC
BstF5I 117 CATCC GGATG(2/0)
BstPI 247 GGTAACC G^GT[ACGT]ACC
BstYI 423 AGATCC [GA]^GATC[CT]
BstZ17I 257 GTATAC GTA^TAC
Cac8I 162 GCGGGC GC[ACGT]^[ACGT]GC
ClaI 179 ATCGAT AT^CGAT
DdeI 104 CTCAG C^T[ACGT]AG
DpnII 424 GATC ^GATC
EcoO65I 247 GGTAACC G^GT[ACGT]ACC
FauI 162 GCGGG CCCGC(4/6)
Fnu4HI 263 GCTGC GC^[ACGT]GC
FokI 117 CATCC GGATG(9/13)
HapII 309 CCGG C^CGG
HpaII 309 CCGG C^CGG
HphI 462 TCACC GGTGA(8/7)
ItaI 263 GCTGC GC^[ACGT]GC
MboI 424 GATC ^GATC
MfeI 288 CAATTG C^AATTG
MflI 423 AGATCC [GA]^GATC[CT]
MslI 153 CATTGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 309 CCGG C^CGG
MunI 288 CAATTG C^AATTG
MwoI 157 GCAATGCGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 309 CCGGG CC^[GC]GG
NdeII 424 GATC ^GATC
NspI 409 GCATGT [GA]CATG^[CT]
PstI 366 CTGCAG CTGCA^G
RsaI 215 GTAC GT^AC
Sau3AI 424 GATC ^GATC
SfaNI 146 GATGC GCATC(5/9)
SfcI 366 CTGCAG C^T[GA][CT]AG
SspI 355 AATATT AAT^ATT
TseI 263 GCTGC G^C[AT]GC
XhoII 423 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
BanII 66 GGGCTC G[GA]GC[CT]^C
101 GGGCTC
BciVI 468 GGATAC GTATCC(6/5)
444 GTATCC
BsaJI 427 CCACGG C^C[ACGT][ACGT]GG
439 CCACGG
BsrDI 153 CATTGC GCAATG(2/0)
157 GCAATG
DsaI 427 CCACGG C^C[GA][CT]GG
439 CCACGG
HinfI 305 GATTC G^A[ACGT]TC
433 GATTC
MboII 39 TCTTC GAAGA(8/7)
210 TCTTC
MseI 170 TTAA T^TAA
236 TTAA
NlaIII 76 CATG CATG^
410 CATG
TaqI 144 TCGA T^CGA
180 TCGA
TfiI 305 GATTC G^A[AT]TC
433 GATTC
Tru9I 170 TTAA T^TAA
236 TTAA
Tsp45I 329 GTGAC ^GT[GC]AC
413 GTGAC
TspRI 106 CAGTG CA[GC]TG[ACGT][ACGT]^
325 CACTG
TthHB8I 144 TCGA T^CGA
180 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
BmyI Bsp1286I BssKI BstNI EcoRII
MaeIII MnlI MvaI NlaIV ScrFI
Tsp509I