Restriction map for
pFN21AE2085
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AocI Aor51HI
ApaI AscI AseI AsnI Asp718I
AvrII BamHI BanI BbeI BbvCI
BciVI BfrI BglII BlnI BlpI
Bpu1102I BsaHI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrDI
BsrFI BsrGI BssHII Bst1107I BstEII
BstPI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DrdI EagI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
EcoT22I FseI Hin1I HindIII HpaI
KasI KpnI MfeI MluI MroI
MunI NaeI NarI NgoMIV NheI
NotI NruI NsiI PacI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
RsrII SalI SapI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SspBI SspI SwaI XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AhdI 2124 GACTCTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1621 GTGCAC G^TGCAC
ApaLI 1621 GTGCAC G^TGCAC
Asp700I 1758 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2124 GACTCTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 247 CTCGAG C^[CT]CG[GA]G
AviII 1871 TGCGCA TGC^GCA
BcgI 1683 GCACAGTGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 647 GCCTCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 1200 GAGACC GGTCTC(1/5)
BsaWI 629 ACCGGA [AT]^CCGG[AT]
BsiEI 1049 CGATCG CG[GA][CT]^CG
BsmBI 1183 GAGACG CGTCTC(1/5)
BsoBI 247 CTCGAG C^[CT]CG[GA]G
BstBI 1500 TTCGAA TT^CGAA
BstF5I 933 GGATG GGATG(2/0)
BstYI 350 AGATCC [GA]^GATC[CT]
DraIII 2266 CACTACGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2124 GACTCTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1762 GAATTC G^AATTC
EcoT14I 1613 CCATGG C^C[AT][AT]GG
FokI 933 GGATG GGATG(9/13)
FspI 1871 TGCGCA TGC^GCA
HgaI 318 GCGTC GACGC(5/10)
HincII 1159 GTCAAC GT[CT]^[GA]AC
HindII 1159 GTCAAC GT[CT]^[GA]AC
MaeIII 1547 GTGAC ^GT[ACGT]AC
MflI 350 AGATCC [GA]^GATC[CT]
NcoI 1613 CCATGG C^CATGG
NdeI 1531 CATATG CA^TATG
NspV 1500 TTCGAA TT^CGAA
PaeR7I 247 CTCGAG C^TCGAG
PvuI 1049 CGATCG CGAT^CG
SacI 253 GAGCTC GAGCT^C
SbfI 669 CCTGCAGG CCTGCA^GG
SfuI 1500 TTCGAA TT^CGAA
SphI 656 GCATGC GCATG^C
Sse8387I 669 CCTGCAGG CCTGCA^GG
StuI 323 AGGCCT AGG^CCT
StyI 1613 CCATGG C^C[AT][AT]GG
Tsp45I 1547 GTGAC ^GT[GC]AC
XcmI 506 CCATCTGGGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 247 CTCGAG C^TCGAG
XhoII 350 AGATCC [GA]^GATC[CT]
XmnI 1758 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AlwI 351 GATCC GGATC(4/5)
1311 GATCC
AspI 334 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1078 GACATGGTC
BalI 1597 TGGCCA TGG^CCA
1844 TGGCCA
BanII 253 GAGCTC G[GA]GC[CT]^C
916 GGGCTC
BbsI 331 GAAGAC GAAGAC(2/6)
367 GAAGAC
BclI 437 TGATCA T^GATCA
443 TGATCA
BcnI 100 CCGGG CC^[GC]GG
981 CCGGG
BfaI 376 CTAG C^TAG
2143 CTAG
BpuAI 331 GAAGAC GAAGAC(2/6)
367 GAAGAC
BspHI 440 TCATGA T^CATGA
1307 TCATGA
BspMI 668 ACCTGC ACCTGC(4/8)
672 GCAGGT
BssSI 199 CACGAG CACGAG(-5/-1)
40 CTCGTG
BstAPI 999 GCAGGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1527 GCAGCATATGC
BstXI 119 CCAAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1463 CCAGCAAGCTGG
DraII 11 AGGCCCC [GA]G^G[ACGT]CC[CT]
1554 AGGCCCT
EcoNI 230 CCTCCATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1164 CCTGATGAAGG
EcoO109I 11 AGGCCCC [GA]G^G[ACGT]CC[CT]
1554 AGGCCCT
FbaI 437 TGATCA T^GATCA
443 TGATCA
HaeII 36 GGCGCT [GA]GCGC^[CT]
1002 GGCGCT
KspI 16 CCGCGG CCGC^GG
71 CCGCGG
MaeI 376 CTAG C^TAG
2143 CTAG
MluNI 1597 TGGCCA TGG^CCA
1844 TGGCCA
MscI 1597 TGGCCA TGG^CCA
1844 TGGCCA
NciI 100 CCGGG CC^[GC]GG
981 CCGGG
PflFI 334 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1078 GACATGGTC
PvuII 970 CAGCTG CAG^CTG
2164 CAGCTG
RcaI 440 TCATGA T^CATGA
1307 TCATGA
SacII 16 CCGCGG CCGC^GG
71 CCGCGG
SmlI 247 CTCGAG C^T[CT][GA]AG
1435 CTCAAG
Tth111I 334 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1078 GACATGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwNI ApoI AspHI AvaII
BbrPI BbvI BmyI BpmI BsaAI
BsaBI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrBI BsrI BssKI BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DsaI EaeI EarI EcoRII
FauI Fnu4HI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MamI MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV NspI PflMI PleI PmaCI
PmlI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
Van91I