Restriction map for
pFN21AE2308
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# NO CUT ENZYMES ( 77 enzymes) #
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AccI AccIII AclI AcsI AflII
AgeI Alw44I ApaLI ApoI AseI
AsnI Asp700I AvrII BamHI BbrPI
BbsI BcgI BclI BfrI BlnI
BpuAI BsaBI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrDI
BsrGI Bst1107I BstBI BstZ17I ClaI
CpoI DraI DrdI EcoRI EcoRV
EcoT22I FbaI HindIII HpaI MamI
MfeI MluI MroI MseI MunI
NdeI NheI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SalI SfuI SnaBI SpeI SspBI
SspI SwaI TfiI Tru9I Tsp509I
XbaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AatII 3303 GACGTC GACGT^C
Acc65I 404 GGTACC G^GTACC
AflIII 2303 ACGTGT A^C[GA][CT]GT
AlwI 3706 GGATC GGATC(4/5)
AocI 2521 CCTCAGG CC^T[ACGT]AGG
AscI 259 GGCGCGCC GG^CGCGCC
Asp718I 404 GGTACC G^GTACC
BbvCI 3826 CCTCAGC CCTCAGC(-5/-2)
BciVI 3039 GTATCC GTATCC(6/5)
BfaI 2615 CTAG C^TAG
BglII 2063 AGATCT A^GATCT
BlpI 3321 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3321 GCTGAGC GC^T[ACGT]AGC
BsaAI 2467 TACGTG [CT]AC^GT[GA]
BsaWI 2730 ACCGGA [AT]^CCGG[AT]
BsrI 2463 CCAGT ACTGG(1/-1)
BstYI 2063 AGATCT [GA]^GATC[CT]
Bsu36I 2521 CCTCAGG CC^T[ACGT]AGG
CelII 3321 GCTGAGC GC^T[ACGT]AGC
Eco81I 2521 CCTCAGG CC^T[ACGT]AGG
EcoNI 2499 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 985 GTCAAC GT[CT]^[GA]AC
HindII 985 GTCAAC GT[CT]^[GA]AC
KpnI 404 GGTACC GGTAC^C
MaeI 2615 CTAG C^TAG
MflI 2063 AGATCT [GA]^GATC[CT]
PaeR7I 217 CTCGAG C^TCGAG
PvuII 2004 CAGCTG CAG^CTG
SapI 888 GCTCTTC GCTCTTC(1/4)
SbfI 3360 CCTGCAGG CCTGCA^GG
Sse8387I 3360 CCTGCAGG CCTGCA^GG
XhoI 217 CTCGAG C^TCGAG
XhoII 2063 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 30 enzymes) #
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AhdI 3660 GACCCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3970 GACTTCTTGTC
AlwNI 1693 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
3278 CAGAAGCTG
Aor51HI 134 AGCGCT AGC^GCT
3883 AGCGCT
AspEI 3660 GACCCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3970 GACTTCTTGTC
AspI 421 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2317 GACGCGGTC
BalI 126 TGGCCA TGG^CCA
3293 TGGCCA
BspHI 725 TCATGA T^CATGA
2474 TCATGA
BstEII 2322 GGTCACC G^GT[ACGT]ACC
3471 GGTCACC
BstPI 2322 GGTCACC G^GT[ACGT]ACC
3471 GGTCACC
BstXI 611 CCAAGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1295 CCATGCCCGTGG
DraIII 85 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
2989 CACCCCGTG
Eam1105I 3660 GACCCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3970 GACTTCTTGTC
EarI 889 CTCTTC CTCTTC(1/4)
3315 GAAGAG
Eco47III 134 AGCGCT AGC^GCT
3883 AGCGCT
EcoO65I 2322 GGTCACC G^GT[ACGT]ACC
3471 GGTCACC
FseI 2565 GGCCGGCC GGCCGG^CC
3649 GGCCGGCC
Ksp632I 889 CTCTTC CTCTTC(1/4)
3315 GAAGAG
MluNI 126 TGGCCA TGG^CCA
3293 TGGCCA
MscI 126 TGGCCA TGG^CCA
3293 TGGCCA
NcoI 1631 CCATGG C^CATGG
2255 CCATGG
PflFI 421 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2317 GACGCGGTC
RcaI 725 TCATGA T^CATGA
2474 TCATGA
ScaI 221 AGTACT AGT^ACT
359 AGTACT
SexAI 422 ACCTGGT A^CC[AT]GGT
980 ACCTGGT
SgrAI 1052 CGCCGGCG C[GA]^CCGG[CT]G
3903 CACCGGCG
SmlI 217 CTCGAG C^T[CT][GA]AG
880 CTCAAG
SphI 2262 GCATGC GCATG^C
2708 GCATGC
StuI 3026 AGGCCT AGG^CCT
3353 AGGCCT
Tth111I 421 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2317 GACGCGGTC
XcmI 1928 CCAGCAGCAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2246 CCAAGCTGTCCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
ApaI AspHI AvaI AvaII AviII
BanI BanII BbeI BbvI BcnI
BglI BmyI BpmI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BssHII BssKI BssSI BstAPI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EclXI Eco52I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
FspI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI KspI MaeII
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIII NlaIV NotI NspI PflMI
PleI PpuMI PstI RsaI SacI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SfiI SfoI SmaI StyI
TaqI TseI Tsp45I TspRI TthHB8I
Van91I XmaCI XmaI