Restriction map for
pFN21AE6369
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I AspEI AspI AvaI AviII
AvrII BalI BamHI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BsgI BsiWI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrFI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstYI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraII DraIII Eam1105I
EarI Eco47III EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HapII HindIII HpaI HpaII Ksp632I
KspI MamI MfeI MflI MluI
MluNI MroI MscI MspI MunI
NaeI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tsp45I Tth111I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Acc65I 60 GGTACC G^GTACC
AccII 597 CGCG CG^CG
AcyI 601 GGCGCC G[GA]^CG[CT]C
AocI 838 CCTGAGG CC^T[ACGT]AGG
Asp718I 60 GGTACC G^GTACC
BanII 844 GAGCTC G[GA]GC[CT]^C
BbeI 601 GGCGCC GGCGC^C
BsaHI 601 GGCGCC G[GA]^CG[CT]C
BseRI 841 GAGGAG GAGGAG(10/8)
BsiEI 190 CGGCCG CG[GA][CT]^CG
BsrDI 663 CATTGC GCAATG(2/0)
BstUI 597 CGCG CG^CG
Bsu36I 838 CCTGAGG CC^T[ACGT]AGG
DrdI 370 GACCTGAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 190 CGGCCG [CT]^GGCC[GA]
EagI 190 CGGCCG C^GGCCG
EclXI 190 CGGCCG C^GGCCG
Eco52I 190 CGGCCG C^GGCCG
Eco81I 838 CCTGAGG CC^T[ACGT]AGG
EcoNI 752 CCTGGTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 377 GCGTC GACGC(5/10)
Hin1I 601 GGCGCC G[GA]^CG[CT]C
HincII 457 GTCGAC GT[CT]^[GA]AC
HindII 457 GTCGAC GT[CT]^[GA]AC
KasI 601 GGCGCC G^GCGCC
KpnI 60 GGTACC GGTAC^C
MaeII 155 ACGT A^CGT
MaeIII 710 GTTAC ^GT[ACGT]AC
MboII 726 TCTTC GAAGA(8/7)
MvnI 597 CGCG CG^CG
NarI 601 GGCGCC GG^CGCC
NcoI 143 CCATGG C^CATGG
NspI 391 ACATGC [GA]CATG^[CT]
PvuII 15 CAGCTG CAG^CTG
SacI 844 GAGCTC GAGCT^C
SalI 457 GTCGAC G^TCGAC
SfcI 876 CTACAG C^T[GA][CT]AG
SfoI 601 GGCGCC GGC^GCC
TspRI 401 CACTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 457 GTCGAC GT^[AC][GT]AC
746 GTCTAC
AcsI 169 AAATTT [GA]^AATT[CT]
962 AAATTC
AlwI 7 GATCC GGATC(4/5)
659 GGATC
ApoI 169 AAATTT [GA]^AATT[CT]
962 AAATTC
BbvI 801 GCAGC GCAGC(8/12)
314 GCTGC
BfaI 175 CTAG C^TAG
734 CTAG
BpmI 283 CTCCAG CTGGAG(16/14)
320 CTGGAG
BsrBI 280 CCGCTC CCGCTC(-3/-3)
868 CCGCTC
BstXI 116 CCATCCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
335 CCAATATCTTGG
CfoI 602 GCGC GCG^C
653 GCGC
DsaI 143 CCATGG C^C[GA][CT]GG
193 CCGTGG
EcoT14I 128 CCTTGG C^C[AT][AT]GG
143 CCATGG
HaeII 601 GGCGCC [GA]GCGC^[CT]
652 AGCGCC
HhaI 602 GCGC GCG^C
653 GCGC
HinP1I 602 GCGC G^CGC
653 GCGC
HphI 132 GGTGA GGTGA(8/7)
860 TCACC
MaeI 175 CTAG C^TAG
734 CTAG
MseI 545 TTAA T^TAA
581 TTAA
MslI 531 CATTGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
621 CATAGCTGTG
MspA1I 15 CAGCTG C[AC]G^C[GT]G
424 CCGCTG
PflMI 446 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
671 CCAAACTGTGG
PleI 460 GACTC GAGTC(4/5)
997 GAGTC
StyI 128 CCTTGG C^C[AT][AT]GG
143 CCATGG
TaqI 356 TCGA T^CGA
458 TCGA
TfiI 230 GAATC G^A[AT]TC
248 GATTC
Tru9I 545 TTAA T^TAA
581 TTAA
TseI 314 GCTGC G^C[AT]GC
801 GCAGC
TthHB8I 356 TCGA T^CGA
458 TCGA
Van91I 446 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
671 CCAAACTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BanI BmyI BsaJI BsiHKAI BsiYI
BslI Bsp1286I BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
FauI Fnu4HI FokI HaeIII HinfI
ItaI MboI MnlI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI SspI Tsp509I