Restriction map for
pFN21AE2244
##################################
# NO CUT ENZYMES ( 161 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfaI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaBI BsaI
BseAI BseRI BsgI BsiEI BsiWI
BsmAI BsmBI BsoBI BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BssHII BssSI Bst1107I BstBI BstUI
BstZ17I Bsu36I CelII CfoI ClaI
CpoI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HaeIII HhaI HinP1I
HindIII HpaI KasI KpnI KspI
MaeI MamI MfeI MluI MluNI
MroI MscI MspA1I MunI MvnI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SfaNI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AcyI 684 GACGCC G[GA]^CG[CT]C
AflII 184 CTTAAG C^TTAAG
AflIII 348 ACGTGT A^C[GA][CT]GT
AgeI 9 ACCGGT A^CCGGT
Asp700I 132 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 281 GAGCTC G[AT]GC[AT]^C
AvaII 666 GGTCC G^G[AT]CC
BanI 12 GGTGCC G^G[CT][GA]CC
BanII 281 GAGCTC G[GA]GC[CT]^C
BfrI 184 CTTAAG C^TTAAG
BsaAI 347 TACGTG [CT]AC^GT[GA]
BsaHI 684 GACGCC G[GA]^CG[CT]C
BsaWI 9 ACCGGT [AT]^CCGG[AT]
BsiHKAI 281 GAGCTC G[AT]GC[AT]^C
BsmI 439 GAATGC GAATGC(1/-1)
Bsp1407I 497 TGTACA T^GTACA
BsrFI 9 ACCGGT [GA]^CCGG[CT]
BsrGI 497 TGTACA T^GTACA
BstAPI 153 GCATATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 406 GGTTACC G^GT[ACGT]ACC
BstPI 406 GGTTACC G^GT[ACGT]ACC
BstXI 608 CCAAGCCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 63 GGATCT [GA]^GATC[CT]
Cfr10I 9 ACCGGT [GA]^CCGG[CT]
Cfr13I 666 GGTCC G^G[ACGT]CC
DraI 448 TTTAAA TTT^AAA
EcoO65I 406 GGTTACC G^GT[ACGT]ACC
EcoT14I 296 CCAAGG C^C[AT][AT]GG
HapII 10 CCGG C^CGG
Hin1I 684 GACGCC G[GA]^CG[CT]C
HincII 736 GTCAAC GT[CT]^[GA]AC
HindII 736 GTCAAC GT[CT]^[GA]AC
HpaII 10 CCGG C^CGG
HphI 681 GGTGA GGTGA(8/7)
MaeII 348 ACGT A^CGT
MflI 63 GGATCT [GA]^GATC[CT]
MspI 10 CCGG C^CGG
NdeI 154 CATATG CA^TATG
PinAI 9 ACCGGT A^CCGGT
PleI 180 GAGTC GAGTC(4/5)
PstI 160 CTGCAG CTGCA^G
SacI 281 GAGCTC GAGCT^C
Sau96I 666 GGTCC G^G[ACGT]CC
SexAI 232 ACCAGGT A^CC[AT]GGT
SfcI 160 CTGCAG C^T[GA][CT]AG
SspBI 497 TGTACA T^GTACA
StyI 296 CCAAGG C^C[AT][AT]GG
TaqI 287 TCGA T^CGA
TthHB8I 287 TCGA T^CGA
XhoII 63 GGATCT [GA]^GATC[CT]
XmnI 132 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AfaI 498 GTAC GT^AC
630 GTAC
AlwI 208 GATCC GGATC(4/5)
63 GGATC
BmyI 13 GTGCCC G[AGT]GC[ACT]^C
281 GAGCTC
BsmFI 342 GGGAC GGGAC(10/14)
667 GTCCC
Bsp1286I 13 GTGCCC G[AGT]GC[ACT]^C
281 GAGCTC
BsrI 337 CCAGT ACTGG(1/-1)
733 CCAGT
Cac8I 245 GCAGGC GC[ACGT]^[ACGT]GC
552 GCAAGC
DdeI 76 CTCAG C^T[ACGT]AG
86 CTAAG
DpnII 64 GATC ^GATC
208 GATC
HgaI 684 GACGC GACGC(5/10)
706 GCGTC
MboI 64 GATC ^GATC
208 GATC
MslI 271 CACCCCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
333 CATACCAGTG
MwoI 153 GCATATGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
245 GCAGGCAAGGC
NdeII 64 GATC ^GATC
208 GATC
RsaI 498 GTAC GT^AC
630 GTAC
Sau3AI 64 GATC ^GATC
208 GATC
SmlI 184 CTTAAG C^T[CT][GA]AG
217 CTCAAG
TfiI 454 GAATC G^A[AT]TC
511 GAATC
Tsp45I 229 GTGAC ^GT[GC]AC
682 GTGAC
TspRI 163 CAGTG CA[GC]TG[ACGT][ACGT]^
338 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AluI BbvI BsaJI BsiYI BslI
BssKI BstF5I BstNI EarI EcoRII
Fnu4HI FokI HinfI ItaI Ksp632I
MaeIII MboII MnlI MseI MvaI
NlaIII NlaIV ScrFI Tru9I TseI
Tsp509I