Restriction map for
pFN21AB5592
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# NO CUT ENZYMES ( 120 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaII
AviII AvrII BalI BamHI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaWI BseAI
BsiWI BspDI BspEI BspHI BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII Eam1105I Eco47III Eco81I
EcoNI EcoO65I FauI FbaI FseI
FspI HincII HindII HpaI KpnI
MaeI MfeI MluI MluNI MroI
MscI MseI MslI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SapI ScaI SexAI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SphI SspI StuI SwaI Tru9I
Tth111I Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AlwNI 1356 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 256 GGGCCC GGGCC^C
BanI 109 GGCGCC G^G[CT][GA]CC
BanII 256 GGGCCC G[GA]GC[CT]^C
BbeI 109 GGCGCC GGCGC^C
BbrPI 229 CACGTG CAC^GTG
BbsI 1105 GAAGAC GAAGAC(2/6)
BbvCI 958 GCTGAGG CCTCAGC(-5/-2)
BcgI 245 GCATGTTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 1105 GAAGAC GAAGAC(2/6)
BsaAI 229 CACGTG [CT]AC^GT[GA]
BsaBI 760 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 192 CGGCCG CG[GA][CT]^CG
BsmBI 695 CGTCTC CGTCTC(1/5)
Bsp1407I 1387 TGTACA T^GTACA
BsrGI 1387 TGTACA T^GTACA
BstYI 993 GGATCT [GA]^GATC[CT]
DraII 256 GGGCCCC [GA]G^G[ACGT]CC[CT]
DrdI 208 GACTGCGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 192 CGGCCG C^GGCCG
EarI 1315 GAAGAG CTCTTC(1/4)
EclXI 192 CGGCCG C^GGCCG
Eco52I 192 CGGCCG C^GGCCG
EcoO109I 256 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRV 661 GATATC GAT^ATC
HaeII 109 GGCGCC [GA]GCGC^[CT]
HindIII 78 AAGCTT A^AGCTT
KasI 109 GGCGCC G^GCGCC
Ksp632I 1315 GAAGAG CTCTTC(1/4)
KspI 1201 CCGCGG CCGC^GG
MamI 760 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 993 GGATCT [GA]^GATC[CT]
NarI 109 GGCGCC GG^CGCC
PleI 136 GAGTC GAGTC(4/5)
PmaCI 229 CACGTG CAC^GTG
PmlI 229 CACGTG CAC^GTG
SacII 1201 CCGCGG CCGC^GG
SbfI 116 CCTGCAGG CCTGCA^GG
SfoI 109 GGCGCC GGC^GCC
Sse8387I 116 CCTGCAGG CCTGCA^GG
SspBI 1387 TGTACA T^GTACA
TaqI 1112 TCGA T^CGA
TthHB8I 1112 TCGA T^CGA
XcmI 354 CCAGGGATGGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 993 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 466 ACATGT A^C[GA][CT]GT
1141 ACATGT
AlwI 766 GATCC GGATC(4/5)
993 GGATC
BpmI 698 CTCCAG CTGGAG(16/14)
1049 CTCCAG
BsaI 289 GAGACC GGTCTC(1/5)
1378 GGTCTC
BseRI 96 GAGGAG GAGGAG(10/8)
961 GAGGAG
BsgI 945 CTGCAC GTGCAG(16/14)
311 GTGCAG
BsmI 550 GAATGC GAATGC(1/-1)
1423 GAATGC
BspLU11I 466 ACATGT A^CATGT
1141 ACATGT
BstAPI 707 GCAAAATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1259 GCATATTGTGC
EaeI 103 TGGCCG [CT]^GGCC[GA]
192 CGGCCG
EcoRI 432 GAATTC G^AATTC
649 GAATTC
EcoT14I 318 CCATGG C^C[AT][AT]GG
588 CCATGG
EcoT22I 552 ATGCAT ATGCA^T
1448 ATGCAT
HphI 742 GGTGA GGTGA(8/7)
665 TCACC
MaeII 230 ACGT A^CGT
694 ACGT
NcoI 318 CCATGG C^CATGG
588 CCATGG
NsiI 552 ATGCAT ATGCA^T
1448 ATGCAT
PstI 117 CTGCAG CTGCA^G
714 CTGCAG
PvuII 65 CAGCTG CAG^CTG
956 CAGCTG
SmaI 128 CCCGGG CCC^GGG
171 CCCGGG
StyI 318 CCATGG C^C[AT][AT]GG
588 CCATGG
TfiI 1236 GAATC G^A[AT]TC
1246 GAATC
XmaCI 128 CCCGGG C^CCGGG
171 CCCGGG
XmaI 128 CCCGGG C^CCGGG
171 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI ApoI AspHI AvaI BbvI
BcnI BmyI BsaHI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EcoRII Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII ItaI
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TseI Tsp45I Tsp509I TspRI