Restriction map for
pFN21AE5751
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# NO CUT ENZYMES ( 114 enzymes) #
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AatII Acc65I AccIII AclI AflII
AflIII AgeI AocI Aor51HI AscI
Asp700I Asp718I AspI AviII AvrII
BamHI BbeI BbvCI BcgI BciVI
BfrI BglII BlnI BlpI Bpu1102I
BsaI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BspEI BspHI BspLU11I
BsrFI BssHII Bst1107I BstBI BstEII
BstPI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I CpoI DraI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV FseI FspI HaeII
HhaI HinP1I KasI KpnI KspI
MflI MluI MroI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PpuMI PshAI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tth111I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 68 enzymes) #
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AccI 268 GTCTAC GT^[AC][GT]AC
AccII 1959 CGCG CG^CG
AcyI 195 GACGCC G[GA]^CG[CT]C
AhdI 281 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 358 GTGCAC G^TGCAC
AlwI 1330 GGATC GGATC(4/5)
AlwNI 1337 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1394 GGGCCC GGGCC^C
ApaLI 358 GTGCAC G^TGCAC
AspEI 281 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 400 CTCGGG C^[CT]CG[GA]G
BalI 707 TGGCCA TGG^CCA
BanI 337 GGCACC G^G[CT][GA]CC
BbrPI 361 CACGTG CAC^GTG
BclI 1001 TGATCA T^GATCA
BcnI 935 CCGGG CC^[GC]GG
BfaI 1387 CTAG C^TAG
BsaAI 361 CACGTG [CT]AC^GT[GA]
BsaBI 996 GATCGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 195 GACGCC G[GA]^CG[CT]C
BsgI 1132 GTGCAG GTGCAG(16/14)
BsmAI 1263 GTCTC GTCTC(1/5)
BsmI 95 GAATGC GAATGC(1/-1)
BsoBI 400 CTCGGG C^[CT]CG[GA]G
Bsp1407I 665 TGTACA T^GTACA
BspDI 1144 ATCGAT AT^CGAT
BspMI 1134 GCAGGT ACCTGC(4/8)
BsrBI 811 GAGCGG CCGCTC(-3/-3)
BsrDI 1041 CATTGC GCAATG(2/0)
BsrGI 665 TGTACA T^GTACA
BssSI 225 CTCGTG CACGAG(-5/-1)
BstAPI 360 GCACGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1959 CGCG CG^CG
BstXI 871 CCAATGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 1144 ATCGAT AT^CGAT
DraII 1394 GGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 361 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 246 GACCATTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 707 TGGCCA [CT]^GGCC[GA]
Eam1105I 281 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 1394 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT22I 1274 ATGCAT ATGCA^T
FauI 1755 CCCGC CCCGC(4/6)
FbaI 1001 TGATCA T^GATCA
HapII 935 CCGG C^CGG
HgaI 195 GACGC GACGC(5/10)
Hin1I 195 GACGCC G[GA]^CG[CT]C
HindIII 584 AAGCTT A^AGCTT
HpaI 1735 GTTAAC GTT^AAC
HpaII 935 CCGG C^CGG
MaeI 1387 CTAG C^TAG
MamI 996 GATCGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 275 CAATTG C^AATTG
MluNI 707 TGGCCA TGG^CCA
MscI 707 TGGCCA TGG^CCA
MspI 935 CCGG C^CGG
MunI 275 CAATTG C^AATTG
MvnI 1959 CGCG CG^CG
NciI 935 CCGGG CC^[GC]GG
NcoI 1106 CCATGG C^CATGG
NsiI 1274 ATGCAT ATGCA^T
PleI 223 GACTC GAGTC(4/5)
PmaCI 361 CACGTG CAC^GTG
PmlI 361 CACGTG CAC^GTG
PstI 916 CTGCAG CTGCA^G
SexAI 1409 ACCAGGT A^CC[AT]GGT
SfiI 1101 GGCCTCCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 665 TGTACA T^GTACA
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# TWO CUT ENZYMES ( 18 enzymes) #
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AseI 307 ATTAAT AT^TAAT
508 ATTAAT
AsnI 307 ATTAAT AT^TAAT
508 ATTAAT
AvaII 1008 GGTCC G^G[AT]CC
1095 GGTCC
BbsI 1162 GTCTTC GAAGAC(2/6)
1789 GTCTTC
BglI 1102 GCCTCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1990 GCCATCATGGC
BpuAI 1162 GTCTTC GAAGAC(2/6)
1789 GTCTTC
BsmFI 403 GGGAC GGGAC(10/14)
1009 GTCCC
DsaI 1106 CCATGG C^C[GA][CT]GG
1874 CCGTGG
EcoRI 569 GAATTC G^AATTC
1810 GAATTC
EcoT14I 651 CCTTGG C^C[AT][AT]GG
1106 CCATGG
HincII 1616 GTCAAC GT[CT]^[GA]AC
1735 GTTAAC
HindII 1616 GTCAAC GT[CT]^[GA]AC
1735 GTTAAC
MaeII 172 ACGT A^CGT
362 ACGT
PshBI 307 ATTAAT AT^TAAT
508 ATTAAT
PvuII 135 CAGCTG CAG^CTG
1689 CAGCTG
SacI 209 GAGCTC GAGCT^C
1486 GAGCTC
SfcI 629 CTGTAG C^T[GA][CT]AG
916 CTGCAG
StyI 651 CCTTGG C^C[AT][AT]GG
1106 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AspHI BanII BbvI BmyI BpmI
BsaJI BsiHKAI BsiYI BslI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EarI EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeIII MboI MboII
MnlI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI