Restriction map for
pFN21AE6770
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# NO CUT ENZYMES ( 115 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BbeI BbrPI BbvCI BcgI BclI
BfaI BglI BlnI BsaAI BsaBI
BsaHI BseAI BspDI BspEI BspLU11I
BssHII BssSI Bst1107I BstBI BstZ17I
ClaI CpoI DraI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoRV
EcoT22I FbaI FseI HaeII HgaI
Hin1I HincII HindII HpaI KasI
KpnI KspI MaeI MamI MfeI
MluI MroI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
StuI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccII 637 CGCG CG^CG
AflII 168 CTTAAG C^TTAAG
AspHI 331 GAGCTC G[AT]GC[AT]^C
AvaI 1339 CCCGAG C^[CT]CG[GA]G
AviII 281 TGCGCA TGC^GCA
BalI 1602 TGGCCA TGG^CCA
BamHI 1560 GGATCC G^GATCC
BanI 112 GGTGCC G^G[CT][GA]CC
BciVI 731 GTATCC GTATCC(6/5)
BfrI 168 CTTAAG C^TTAAG
BglII 1051 AGATCT A^GATCT
BlpI 1061 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1061 GCTCAGC GC^T[ACGT]AGC
BsaI 1622 GGTCTC GGTCTC(1/5)
BsaWI 1568 ACCGGA [AT]^CCGG[AT]
BseRI 1255 GAGGAG GAGGAG(10/8)
BsiEI 1566 CGACCG CG[GA][CT]^CG
BsiHKAI 331 GAGCTC G[AT]GC[AT]^C
BsiWI 107 CGTACG C^GTACG
BsmBI 602 GAGACG CGTCTC(1/5)
BsmFI 1517 GTCCC GGGAC(10/14)
BsoBI 1339 CCCGAG C^[CT]CG[GA]G
Bsp1407I 392 TGTACA T^GTACA
BsrBI 1059 CCGCTC CCGCTC(-3/-3)
BsrFI 1145 ACCGGC [GA]^CCGG[CT]
BsrGI 392 TGTACA T^GTACA
BstAPI 1493 GCAGTGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 630 GGTTACC G^GT[ACGT]ACC
BstPI 630 GGTTACC G^GT[ACGT]ACC
BstUI 637 CGCG CG^CG
BstXI 203 CCACAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1061 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1145 ACCGGC [GA]^CCGG[CT]
DraIII 855 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1144 GACCGGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1520 CCACGG C^C[GA][CT]GG
EaeI 1602 TGGCCA [CT]^GGCC[GA]
EcoO65I 630 GGTTACC G^GT[ACGT]ACC
EcoRI 402 GAATTC G^AATTC
FspI 281 TGCGCA TGC^GCA
HindIII 1137 AAGCTT A^AGCTT
MluNI 1602 TGGCCA TGG^CCA
MscI 1602 TGGCCA TGG^CCA
MvnI 637 CGCG CG^CG
PvuII 520 CAGCTG CAG^CTG
SacI 331 GAGCTC GAGCT^C
SspBI 392 TGTACA T^GTACA
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# TWO CUT ENZYMES ( 25 enzymes) #
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AflIII 1084 ACACGT A^C[GA][CT]GT
1511 ACGTGT
AocI 181 CCTCAGG CC^T[ACGT]AGG
1105 CCTGAGG
BcnI 62 CCGGG CC^[GC]GG
611 CCCGG
BsgI 198 CTGCAC GTGCAG(16/14)
1659 GTGCAG
BsmI 342 GAATGC GAATGC(1/-1)
1261 GAATGC
BstYI 1051 AGATCT [GA]^GATC[CT]
1560 GGATCC
Bsu36I 181 CCTCAGG CC^T[ACGT]AGG
1105 CCTGAGG
CfoI 282 GCGC GCG^C
638 GCGC
Eco81I 181 CCTCAGG CC^T[ACGT]AGG
1105 CCTGAGG
EcoT14I 116 CCAAGG C^C[AT][AT]GG
707 CCAAGG
HhaI 282 GCGC GCG^C
638 GCGC
HinP1I 282 GCGC G^CGC
638 GCGC
MflI 1051 AGATCT [GA]^GATC[CT]
1560 GGATCC
MspA1I 520 CAGCTG C[AC]G^C[GT]G
1238 CAGCGG
NciI 62 CCGGG CC^[GC]GG
611 CCCGG
PleI 1582 GACTC GAGTC(4/5)
925 GAGTC
PpuMI 419 AGGACCT [GA]G^G[AT]CC[CT]
655 AGGACCT
PshAI 657 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1510 GACGTGTGTC
SfaNI 750 GATGC GCATC(5/9)
877 GATGC
SmlI 168 CTTAAG C^T[CT][GA]AG
589 CTCAAG
SphI 386 GCATGC GCATG^C
989 GCATGC
StyI 116 CCAAGG C^C[AT][AT]GG
707 CCAAGG
TaqI 242 TCGA T^CGA
851 TCGA
TthHB8I 242 TCGA T^CGA
851 TCGA
XhoII 1051 AGATCT [GA]^GATC[CT]
1560 GGATCC
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# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BanII BbsI
BbvI BmyI BpmI BpuAI BsaJI
BsiYI BslI BsmAI Bsp1286I BspHI
BspMI BsrDI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MnlI MseI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PstI RcaI
RsaI Sau3AI Sau96I ScrFI SfcI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI