Restriction map for
pFN21AE1984
##################################
# NO CUT ENZYMES ( 154 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BanI BbeI BbrPI BbvCI
BcgI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaHI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspMI BsrBI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI ClaI CpoI
DraII DraIII EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HaeII HaeIII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI KasI KpnI KspI MaeII
MluI MluNI MroI MscI MspA1I
MvnI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI StuI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AciI 1127 CCGC CCGC(-3/-1)
AflII 246 CTTAAG C^TTAAG
AflIII 1573 ACATGT A^C[GA][CT]GT
AvaII 1034 GGACC G^G[AT]CC
BamHI 53 GGATCC G^GATCC
BanII 714 GAGCCC G[GA]GC[CT]^C
BbsI 277 GAAGAC GAAGAC(2/6)
BciVI 1527 GGATAC GTATCC(6/5)
BcnI 637 CCCGG CC^[GC]GG
BfrI 246 CTTAAG C^TTAAG
BmyI 714 GAGCCC G[AGT]GC[ACT]^C
BpuAI 277 GAAGAC GAAGAC(2/6)
BsaBI 223 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1286I 714 GAGCCC G[AGT]GC[ACT]^C
BspHI 348 TCATGA T^CATGA
BspLU11I 1573 ACATGT A^CATGT
BsrFI 1350 GCCGGT [GA]^CCGG[CT]
BstYI 53 GGATCC [GA]^GATC[CT]
Cfr10I 1350 GCCGGT [GA]^CCGG[CT]
Cfr13I 1034 GGACC G^G[ACGT]CC
FauI 1126 CCCGC CCCGC(4/6)
HindIII 236 AAGCTT A^AGCTT
MamI 223 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 836 CAATTG C^AATTG
MflI 53 GGATCC [GA]^GATC[CT]
MunI 836 CAATTG C^AATTG
NciI 637 CCCGG CC^[GC]GG
NdeI 1420 CATATG CA^TATG
RcaI 348 TCATGA T^CATGA
Sau96I 1034 GGACC G^G[ACGT]CC
SpeI 799 ACTAGT A^CTAGT
TaqI 258 TCGA T^CGA
TthHB8I 258 TCGA T^CGA
XcmI 976 CCAATTAAAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 53 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Asp700I 220 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
914 GAACAAATTC
BclI 180 TGATCA T^GATCA
345 TGATCA
BsaI 1330 GAGACC GGTCTC(1/5)
989 GGTCTC
BsrDI 467 CATTGC GCAATG(2/0)
1395 CATTGC
Cac8I 971 GCTCGC GC[ACGT]^[ACGT]GC
1651 GCAAGC
DraI 63 TTTAAA TTT^AAA
133 TTTAAA
DrdI 465 GACATTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1203 GACTGCCATGTC
DsaI 1130 CCATGG C^C[GA][CT]GG
1182 CCATGG
EarI 476 CTCTTC CTCTTC(1/4)
301 GAAGAG
FbaI 180 TGATCA T^GATCA
345 TGATCA
HapII 638 CCGG C^CGG
1351 CCGG
HpaII 638 CCGG C^CGG
1351 CCGG
Ksp632I 476 CTCTTC CTCTTC(1/4)
301 GAAGAG
MspI 638 CCGG C^CGG
1351 CCGG
MwoI 785 GCTTGGGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1129 GCCATGGATGC
NcoI 1130 CCATGG C^CATGG
1182 CCATGG
NspI 1427 GCATGT [GA]CATG^[CT]
1573 ACATGT
SfcI 203 CTGTAG C^T[GA][CT]AG
1313 CTATAG
SspI 1099 AATATT AAT^ATT
1519 AATATT
TfiI 16 GAATC G^A[AT]TC
1044 GATTC
XmnI 220 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
914 GAACAAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbvI BfaI BpmI BsaJI BsiYI
BslI BsmAI BsrI BssKI BstF5I
BstNI DdeI DpnII EcoRII EcoT14I
Fnu4HI FokI HinfI HphI ItaI
MaeI MaeIII MboI MboII MnlI
MseI MslI MvaI NdeII NlaIII
NlaIV PleI RsaI Sau3AI ScrFI
SfaNI SmlI StyI Tru9I TseI
Tsp45I Tsp509I TspRI