################################## # NO CUT ENZYMES ( 154 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BanI BbeI BbrPI BbvCI BcgI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraII DraIII EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII HaeIII HgaI HhaI Hin1I HinP1I HincII HindII HpaI KasI KpnI KspI MaeII MluI MluNI MroI MscI MspA1I MvnI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI StuI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AciI 1127 CCGC CCGC(-3/-1) AflII 246 CTTAAG C^TTAAG AflIII 1573 ACATGT A^C[GA][CT]GT AvaII 1034 GGACC G^G[AT]CC BamHI 53 GGATCC G^GATCC BanII 714 GAGCCC G[GA]GC[CT]^C BbsI 277 GAAGAC GAAGAC(2/6) BciVI 1527 GGATAC GTATCC(6/5) BcnI 637 CCCGG CC^[GC]GG BfrI 246 CTTAAG C^TTAAG BmyI 714 GAGCCC G[AGT]GC[ACT]^C BpuAI 277 GAAGAC GAAGAC(2/6) BsaBI 223 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC Bsp1286I 714 GAGCCC G[AGT]GC[ACT]^C BspHI 348 TCATGA T^CATGA BspLU11I 1573 ACATGT A^CATGT BsrFI 1350 GCCGGT [GA]^CCGG[CT] BstYI 53 GGATCC [GA]^GATC[CT] Cfr10I 1350 GCCGGT [GA]^CCGG[CT] Cfr13I 1034 GGACC G^G[ACGT]CC FauI 1126 CCCGC CCCGC(4/6) HindIII 236 AAGCTT A^AGCTT MamI 223 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 836 CAATTG C^AATTG MflI 53 GGATCC [GA]^GATC[CT] MunI 836 CAATTG C^AATTG NciI 637 CCCGG CC^[GC]GG NdeI 1420 CATATG CA^TATG RcaI 348 TCATGA T^CATGA Sau96I 1034 GGACC G^G[ACGT]CC SpeI 799 ACTAGT A^CTAGT TaqI 258 TCGA T^CGA TthHB8I 258 TCGA T^CGA XcmI 976 CCAATTAAAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 53 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### Asp700I 220 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 914 GAACAAATTC BclI 180 TGATCA T^GATCA 345 TGATCA BsaI 1330 GAGACC GGTCTC(1/5) 989 GGTCTC BsrDI 467 CATTGC GCAATG(2/0) 1395 CATTGC Cac8I 971 GCTCGC GC[ACGT]^[ACGT]GC 1651 GCAAGC DraI 63 TTTAAA TTT^AAA 133 TTTAAA DrdI 465 GACATTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1203 GACTGCCATGTC DsaI 1130 CCATGG C^C[GA][CT]GG 1182 CCATGG EarI 476 CTCTTC CTCTTC(1/4) 301 GAAGAG FbaI 180 TGATCA T^GATCA 345 TGATCA HapII 638 CCGG C^CGG 1351 CCGG HpaII 638 CCGG C^CGG 1351 CCGG Ksp632I 476 CTCTTC CTCTTC(1/4) 301 GAAGAG MspI 638 CCGG C^CGG 1351 CCGG MwoI 785 GCTTGGGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1129 GCCATGGATGC NcoI 1130 CCATGG C^CATGG 1182 CCATGG NspI 1427 GCATGT [GA]CATG^[CT] 1573 ACATGT SfcI 203 CTGTAG C^T[GA][CT]AG 1313 CTATAG SspI 1099 AATATT AAT^ATT 1519 AATATT TfiI 16 GAATC G^A[AT]TC 1044 GATTC XmnI 220 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 914 GAACAAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 48 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI BbvI BfaI BpmI BsaJI BsiYI BslI BsmAI BsrI BssKI BstF5I BstNI DdeI DpnII EcoRII EcoT14I Fnu4HI FokI HinfI HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MslI MvaI NdeII NlaIII NlaIV PleI RsaI Sau3AI ScrFI SfaNI SmlI StyI Tru9I TseI Tsp45I Tsp509I TspRI