Restriction map for
pFN21AE1828
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# NO CUT ENZYMES ( 156 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AviII AvrII BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BcnI BfaI BfrI BglI
BlnI BmyI BpmI BpuAI BsaAI
BsaHI BsaWI BseAI BseRI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1286I BspDI BspEI BspHI BspMI
BsrBI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HpaI HpaII KasI KpnI Ksp632I
KspI MaeI MluI MroI MspA1I
MspI MvnI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshBI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
StuI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AciI 714 GCGG CCGC(-3/-1)
AclI 220 AACGTT AA^CGTT
AfaI 366 GTAC GT^AC
AflIII 362 ACATGT A^C[GA][CT]GT
Asp700I 219 GAACGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 239 TGGCCA TGG^CCA
BanI 61 GGCACC G^G[CT][GA]CC
BglII 249 AGATCT A^GATCT
BlpI 586 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 586 GCTAAGC GC^T[ACGT]AGC
BsaBI 489 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 320 GAGACC GGTCTC(1/5)
BsmAI 320 GAGAC GTCTC(1/5)
BsmFI 697 GTCCC GGGAC(10/14)
Bsp1407I 365 TGTACA T^GTACA
BspLU11I 362 ACATGT A^CATGT
BsrDI 415 GCAATG GCAATG(2/0)
BsrGI 365 TGTACA T^GTACA
BsrI 870 ACTGG ACTGG(1/-1)
CelII 586 GCTAAGC GC^T[ACGT]AGC
DraII 695 AGGTCCC [GA]G^G[ACGT]CC[CT]
EaeI 239 TGGCCA [CT]^GGCC[GA]
EcoO109I 695 AGGTCCC [GA]G^G[ACGT]CC[CT]
FauI 714 GCGGG CCCGC(4/6)
HincII 358 GTCAAC GT[CT]^[GA]AC
HindII 358 GTCAAC GT[CT]^[GA]AC
HindIII 854 AAGCTT A^AGCTT
MaeII 221 ACGT A^CGT
MamI 489 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 239 TGGCCA TGG^CCA
MscI 239 TGGCCA TGG^CCA
MslI 210 CACATCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 483 CATATG CA^TATG
PleI 155 GAGTC GAGTC(4/5)
PpuMI 695 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 944 GACTAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 220 AACGTT AA^CGTT
RsaI 366 GTAC GT^AC
SbfI 329 CCTGCAGG CCTGCA^GG
Sse8387I 329 CCTGCAGG CCTGCA^GG
SspBI 365 TGTACA T^GTACA
SspI 141 AATATT AAT^ATT
XmnI 219 GAACGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwNI 84 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
651 CAGCATCTG
AvaII 696 GGTCC G^G[AT]CC
800 GGACC
BamHI 467 GGATCC G^GATCC
488 GGATCC
BclI 668 TGATCA T^GATCA
758 TGATCA
BsaJI 614 CCTTGG C^C[ACGT][ACGT]GG
875 CCTTGG
BsgI 90 CTGCAC GTGCAG(16/14)
953 CTGCAC
BstAPI 83 GCAGGAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
883 GCAGCCAATGC
BstF5I 29 CATCC GGATG(2/0)
970 CATCC
Cfr13I 696 GGTCC G^G[ACGT]CC
800 GGACC
EcoT14I 614 CCTTGG C^C[AT][AT]GG
875 CCTTGG
FbaI 668 TGATCA T^GATCA
758 TGATCA
FokI 29 CATCC GGATG(9/13)
970 CATCC
MaeIII 445 GTCAC ^GT[ACGT]AC
550 GTCAC
MboII 764 TCTTC GAAGA(8/7)
767 TCTTC
MseI 685 TTAA T^TAA
935 TTAA
NspI 362 ACATGT [GA]CATG^[CT]
441 GCATGT
Sau96I 696 GGTCC G^G[ACGT]CC
800 GGACC
SfaNI 452 GCATC GCATC(5/9)
653 GCATC
StyI 614 CCTTGG C^C[AT][AT]GG
875 CCTTGG
TfiI 570 GAATC G^A[AT]TC
703 GAATC
Tru9I 685 TTAA T^TAA
935 TTAA
Tsp45I 445 GTCAC ^GT[GC]AC
550 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AcsI AluI AlwI ApoI BbvI
BsiYI BslI BssKI BstNI BstYI
Cac8I DdeI DpnII EcoRII Fnu4HI
HaeIII HinfI HphI ItaI MboI
MfeI MflI MnlI MunI MvaI
MwoI NdeII NlaIII NlaIV PstI
Sau3AI ScrFI SfcI TseI Tsp509I
TspRI XhoII