Restriction map for
pFN21AB5802
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# NO CUT ENZYMES ( 141 enzymes) #
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AatII AccII AccIII AclI AcyI
AflII AflIII AhdI Alw44I AlwNI
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I AspEI AspI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BciVI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BseAI BseRI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstUI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT14I FauI FseI
FspI HaeII HgaI Hin1I KasI
KspI MamI MfeI MflI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI PacI
PaeR7I PflFI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SmlI SnaBI Sse8387I SspBI SspI
StuI StyI SwaI Tth111I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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Acc65I 413 GGTACC G^GTACC
AcsI 1290 GAATTC [GA]^AATT[CT]
AgeI 416 ACCGGT A^CCGGT
ApaI 1076 GGGCCC GGGCC^C
ApoI 1290 GAATTC [GA]^AATT[CT]
Asp718I 413 GGTACC G^GTACC
BclI 752 TGATCA T^GATCA
BsaWI 416 ACCGGT [AT]^CCGG[AT]
BsgI 443 GTGCAG GTGCAG(16/14)
BsiEI 889 CGGTCG CG[GA][CT]^CG
BsmAI 194 GTCTC GTCTC(1/5)
BstBI 654 TTCGAA TT^CGAA
BstXI 503 CCATCTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 500 GCGC GCG^C
DsaI 476 CCACGG C^C[GA][CT]GG
EaeI 1034 TGGCCG [CT]^GGCC[GA]
EcoRI 1290 GAATTC G^AATTC
EcoT22I 122 ATGCAT ATGCA^T
FbaI 752 TGATCA T^GATCA
HhaI 500 GCGC GCG^C
HinP1I 500 GCGC G^CGC
HindIII 436 AAGCTT A^AGCTT
HpaI 396 GTTAAC GTT^AAC
KpnI 413 GGTACC GGTAC^C
MspA1I 886 CAGCGG C[AC]G^C[GT]G
NsiI 122 ATGCAT ATGCA^T
NspI 961 GCATGC [GA]CATG^[CT]
NspV 654 TTCGAA TT^CGAA
PflMI 631 CCAGTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 416 ACCGGT A^CCGGT
PpuMI 910 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 313 CTGCAG CTGCA^G
SacI 33 GAGCTC GAGCT^C
SalI 139 GTCGAC G^TCGAC
SfuI 654 TTCGAA TT^CGAA
SpeI 1131 ACTAGT A^CTAGT
SphI 961 GCATGC GCATG^C
Van91I 631 CCAGTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 139 GTCGAC GT^[AC][GT]AC
1135 GTCTAC
AfaI 414 GTAC GT^AC
1194 GTAC
AlwI 894 GATCC GGATC(4/5)
1100 GGATC
AspHI 33 GAGCTC G[AT]GC[AT]^C
466 GTGCTC
BanII 33 GAGCTC G[GA]GC[CT]^C
1076 GGGCCC
BbsI 867 GTCTTC GAAGAC(2/6)
982 GTCTTC
BpuAI 867 GTCTTC GAAGAC(2/6)
982 GTCTTC
BsaJI 476 CCACGG C^C[ACGT][ACGT]GG
907 CCAGGG
BsiHKAI 33 GAGCTC G[AT]GC[AT]^C
466 GTGCTC
BsmFI 1278 GGGAC GGGAC(10/14)
297 GTCCC
BsmI 318 GAATGC GAATGC(1/-1)
1353 GCATTC
BsrFI 416 ACCGGT [GA]^CCGG[CT]
1242 ACCGGC
Cac8I 434 GCAAGC GC[ACGT]^[ACGT]GC
961 GCATGC
Cfr10I 416 ACCGGT [GA]^CCGG[CT]
1242 ACCGGC
DdeI 569 CTAAG C^T[ACGT]AG
854 CTCAG
HapII 417 CCGG C^CGG
1243 CCGG
HpaII 417 CCGG C^CGG
1243 CCGG
MaeII 464 ACGT A^CGT
521 ACGT
MaeIII 342 GTCAC ^GT[ACGT]AC
777 GTCAC
MspI 417 CCGG C^CGG
1243 CCGG
PleI 142 GACTC GAGTC(4/5)
1051 GAGTC
RsaI 414 GTAC GT^AC
1194 GTAC
SapI 468 GCTCTTC GCTCTTC(1/4)
879 GCTCTTC
TfiI 534 GAATC G^A[AT]TC
652 GATTC
Tsp45I 342 GTCAC ^GT[GC]AC
777 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AluI AvaII BanI BbvI
BfaI BmyI BpmI BsiYI BslI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI Cfr13I DpnII DraII DrdI
EarI EcoO109I EcoRII Fnu4HI FokI
HaeIII HincII HindII HinfI HphI
ItaI Ksp632I MaeI MboI MboII
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI Tru9I
TseI Tsp509I TspRI TthHB8I