Restriction map for
pFN21AB7061
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# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AlwNI AscI AseI
AsnI Asp718I AvaII AviII BamHI
BbrPI BbvCI BcgI BciVI BclI
BfrI BsaAI BsaBI BsaI BsaWI
BseAI BsiWI BsmBI BspDI BspEI
BspLU11I BspMI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
ClaI CpoI DraI DrdI EcoNI
EcoO65I EcoRV EcoT22I FbaI FseI
FspI KpnI MamI MfeI MroI
MunI NaeI NgoMIV NheI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SalI
SbfI SexAI SfuI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AflIII 473 ACGCGT A^C[GA][CT]GT
AhdI 1330 GACAATAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 66 GTGCAC G^TGCAC
AocI 13 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1823 AGCGCT AGC^GCT
ApaLI 66 GTGCAC G^TGCAC
Asp700I 695 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1330 GACAATAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1170 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 748 CCTAGG C^CTAGG
BbsI 1674 GAAGAC GAAGAC(2/6)
BglII 554 AGATCT A^GATCT
BlnI 748 CCTAGG C^CTAGG
BlpI 1582 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1582 GCTCAGC GC^T[ACGT]AGC
BpuAI 1674 GAAGAC GAAGAC(2/6)
BsmAI 978 GAGAC GTCTC(1/5)
BsmI 1029 GCATTC GAATGC(1/-1)
Bsp1407I 1683 TGTACA T^GTACA
BsrFI 405 GCCGGT [GA]^CCGG[CT]
BsrGI 1683 TGTACA T^GTACA
BssSI 63 CTCGTG CACGAG(-5/-1)
BstYI 554 AGATCT [GA]^GATC[CT]
Bsu36I 13 CCTCAGG CC^T[ACGT]AGG
CelII 1582 GCTCAGC GC^T[ACGT]AGC
Cfr10I 405 GCCGGT [GA]^CCGG[CT]
DraIII 1925 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1330 GACAATAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 755 GAAGAG CTCTTC(1/4)
Eco47III 1823 AGCGCT AGC^GCT
Eco81I 13 CCTCAGG CC^T[ACGT]AGG
HindIII 1917 AAGCTT A^AGCTT
HpaI 1600 GTTAAC GTT^AAC
Ksp632I 755 GAAGAG CTCTTC(1/4)
MaeII 1484 ACGT A^CGT
MflI 554 AGATCT [GA]^GATC[CT]
MluI 473 ACGCGT A^CGCGT
NcoI 767 CCATGG C^CATGG
NdeI 1274 CATATG CA^TATG
PflFI 1170 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 721 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1595 CTGCAG CTGCA^G
PvuII 1746 CAGCTG CAG^CTG
SacI 522 GAGCTC GAGCT^C
SapI 755 GAAGAGC GCTCTTC(1/4)
ScaI 964 AGTACT AGT^ACT
SfcI 1595 CTGCAG C^T[GA][CT]AG
SfiI 291 GGCCGCCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 404 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 382 CCCGGG CCC^GGG
SspBI 1683 TGTACA T^GTACA
TspRI 1929 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 1170 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 721 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 554 AGATCT [GA]^GATC[CT]
XmaCI 382 CCCGGG C^CCGGG
XmaI 382 CCCGGG C^CCGGG
XmnI 695 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwI 1441 GATCC GGATC(4/5)
865 GGATC
ApaI 333 GGGCCC GGGCC^C
1494 GGGCCC
AspHI 66 GTGCAC G[AT]GC[AT]^C
522 GAGCTC
AvaI 382 CCCGGG C^[CT]CG[GA]G
1647 CTCGGG
BalI 837 TGGCCA TGG^CCA
1278 TGGCCA
BglI 292 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
378 GCCGCCCGGGC
BsgI 352 CTGCAC GTGCAG(16/14)
676 GTGCAG
BsiHKAI 66 GTGCAC G[AT]GC[AT]^C
522 GAGCTC
BsoBI 382 CCCGGG C^[CT]CG[GA]G
1647 CTCGGG
BspHI 209 TCATGA T^CATGA
1487 TCATGA
BsrBI 430 CCGCTC CCGCTC(-3/-3)
735 GAGCGG
BsrDI 908 GCAATG GCAATG(2/0)
1751 GCAATG
DraII 332 AGGGCCC [GA]G^G[ACGT]CC[CT]
1494 GGGCCCT
EcoO109I 332 AGGGCCC [GA]G^G[ACGT]CC[CT]
1494 GGGCCCT
EcoRI 630 GAATTC G^AATTC
1242 GAATTC
HincII 1512 GTCAAC GT[CT]^[GA]AC
1600 GTTAAC
HindII 1512 GTCAAC GT[CT]^[GA]AC
1600 GTTAAC
KspI 161 CCGCGG CCGC^GG
296 CCGCGG
MluNI 837 TGGCCA TGG^CCA
1278 TGGCCA
MscI 837 TGGCCA TGG^CCA
1278 TGGCCA
NotI 289 GCGGCCGC GC^GGCCGC
298 GCGGCCGC
NspI 1578 ACATGC [GA]CATG^[CT]
1811 GCATGT
RcaI 209 TCATGA T^CATGA
1487 TCATGA
SacII 161 CCGCGG CCGC^GG
296 CCGCGG
TfiI 1551 GAATC G^A[AT]TC
1843 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI ApoI BanI BanII BbeI
BbvI BcnI BfaI BmyI BpmI
BsaHI BsaJI BseRI BsiEI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EaeI
EagI EclXI Eco52I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
MaeI MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfoI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TthHB8I XcmI