##################################
# NO CUT ENZYMES (  218 enzymes) #
##################################

Acc65I      AccI        AccII       AccIII      AclI        
AflII       AflIII      AgeI        AhdI        AluI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BcnI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaI        BsaWI       BseAI       BseRI       
BsiEI       BsiHKAI     BsiWI       BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BsrI        BssHII      BssKI       BssSI       
Bst1107I    BstBI       BstEII      BstF5I      BstNI       
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
ClaI        CpoI        DraII       DraIII      DrdI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRII      EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        FokI        FseI        
FspI        HaeII       HaeIII      HapII       HgaI        
HhaI        HinP1I      HincII      HindII      HindIII     
HinfI       HpaI        HpaII       HphI        KasI        
KpnI        Ksp632I     KspI        MaeIII      MamI        
MfeI        MflI        MluI        MluNI       MnlI        
MroI        MscI        MslI        MspA1I      MspI        
MunI        MvaI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScrFI       SexAI       
SfaNI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TfiI        Tsp45I      Tth111I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   25 enzymes) #
###################################

AatII          30  GACGTC               GACGT^C
AciI          222  GCGG                 CCGC(-3/-1)
AcsI          115  GAATTT               [GA]^AATT[CT]
AcyI           30  GACGTC               G[GA]^CG[CT]C
ApoI          115  GAATTT               [GA]^AATT[CT]
AvaII          36  GGACC                G^G[AT]CC
BfaI          233  CTAG                 C^TAG
BsaHI          30  GACGTC               G[GA]^CG[CT]C
BsaJI          40  CCACGG               C^C[ACGT][ACGT]GG
BsgI          176  CTGCAC               GTGCAG(16/14)
Cfr13I         36  GGACC                G^G[ACGT]CC
DdeI           61  CTCAG                C^T[ACGT]AG
DraI          118  TTTAAA               TTT^AAA
DsaI           40  CCACGG               C^C[GA][CT]GG
Hin1I          30  GACGTC               G[GA]^CG[CT]C
MaeI          233  CTAG                 C^TAG
MaeII          31  ACGT                 A^CGT
MboII         112  GAAGA                GAAGA(8/7)
NlaIII        208  CATG                 CATG^
NlaIV          36  GGACCC               GG[ACGT]^[ACGT]CC
Sau96I         36  GGACC                G^G[ACGT]CC
ScaI           57  AGTACT               AGT^ACT
TaqI            4  TCGA                 T^CGA
TspRI          63  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I         4  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (    7 enzymes) #
###################################

BstAPI        133  GCAACAAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              178  GCACAGACTGC         
DpnII          21  GATC                 ^GATC
              238  GATC                
MboI           21  GATC                 ^GATC
              238  GATC                
MwoI          133  GCAACAAGTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              178  GCACAGACTGC         
NdeII          21  GATC                 ^GATC
              238  GATC                
Sau3AI         21  GATC                 ^GATC
              238  GATC                
Tsp509I       116  AATT                 ^AATT
              144  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (    8 enzymes) #
#########################################

AfaI        BbvI        Fnu4HI      ItaI        MseI        
RsaI        Tru9I       TseI