Restriction map for
pFN21AE5758
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
ApaI ApaLI AscI AspEI AspI
AvaI AviII AvrII BalI BbeI
BbrPI BcgI BciVI BcnI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsiYI BslI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DrdI
EaeI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoRI FauI FseI
FspI HapII HindIII HpaII KasI
KspI MamI MluI MluNI MroI
MscI MspI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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Acc65I 700 GGTACC G^GTACC
AccII 1465 CGCG CG^CG
AciI 1464 CCGC CCGC(-3/-1)
AcyI 352 GACGCC G[GA]^CG[CT]C
AlwNI 2096 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 162 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 700 GGTACC G^GTACC
BamHI 388 GGATCC G^GATCC
BanII 1567 GAGCTC G[GA]GC[CT]^C
BbvCI 1536 CCTCAGC CCTCAGC(-5/-2)
BglII 80 AGATCT A^GATCT
BsaHI 352 GACGCC G[GA]^CG[CT]C
BsmI 486 GAATGC GAATGC(1/-1)
BspHI 1841 TCATGA T^CATGA
BstUI 1465 CGCG CG^CG
BstXI 297 CCATGTTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 1752 TTTAAA TTT^AAA
DraIII 848 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 636 CCATGG C^C[GA][CT]GG
EcoRV 442 GATATC GAT^ATC
Hin1I 352 GACGCC G[GA]^CG[CT]C
HpaI 46 GTTAAC GTT^AAC
KpnI 700 GGTACC GGTAC^C
MaeII 467 ACGT A^CGT
MfeI 983 CAATTG C^AATTG
MunI 983 CAATTG C^AATTG
MvnI 1465 CGCG CG^CG
NcoI 636 CCATGG C^CATGG
RcaI 1841 TCATGA T^CATGA
SacI 1567 GAGCTC GAGCT^C
SapI 1672 GCTCTTC GCTCTTC(1/4)
SexAI 1734 ACCTGGT A^CC[AT]GGT
SpeI 1633 ACTAGT A^CTAGT
XbaI 2305 TCTAGA T^CTAGA
XcmI 591 CCATTATCCTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 162 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Aor51HI 341 AGCGCT AGC^GCT
1520 AGCGCT
AspHI 623 GTGCTC G[AT]GC[AT]^C
1567 GAGCTC
BbsI 2309 GAAGAC GAAGAC(2/6)
1375 GTCTTC
BclI 1083 TGATCA T^GATCA
1911 TGATCA
BpuAI 2309 GAAGAC GAAGAC(2/6)
1375 GTCTTC
BsiHKAI 623 GTGCTC G[AT]GC[AT]^C
1567 GAGCTC
BsmBI 794 GAGACG CGTCTC(1/5)
964 GAGACG
BssSI 848 CACGAG CACGAG(-5/-1)
2075 CACGAG
BstEII 2178 GGTCACC G^GT[ACGT]ACC
2187 GGTCACC
BstPI 2178 GGTCACC G^GT[ACGT]ACC
2187 GGTCACC
Eco47III 341 AGCGCT AGC^GCT
1520 AGCGCT
EcoO65I 2178 GGTCACC G^GT[ACGT]ACC
2187 GGTCACC
EcoT22I 1232 ATGCAT ATGCA^T
1413 ATGCAT
FbaI 1083 TGATCA T^GATCA
1911 TGATCA
HgaI 352 GACGC GACGC(5/10)
966 GACGC
HincII 46 GTTAAC GT[CT]^[GA]AC
522 GTTGAC
HindII 46 GTTAAC GT[CT]^[GA]AC
522 GTTGAC
MslI 298 CATGTTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2262 CACACCCGTG
MspA1I 755 CAGCTG C[AC]G^C[GT]G
1391 CAGCTG
MwoI 308 GCACTCTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
886 GCTTCCGAAGC
NsiI 1232 ATGCAT ATGCA^T
1413 ATGCAT
PleI 937 GAGTC GAGTC(4/5)
1373 GAGTC
PpuMI 1088 AGGTCCT [GA]G^G[AT]CC[CT]
2097 AGGTCCT
PstI 1285 CTGCAG CTGCA^G
1553 CTGCAG
PvuII 755 CAGCTG CAG^CTG
1391 CAGCTG
SfcI 1285 CTGCAG C^T[GA][CT]AG
1553 CTGCAG
TaqI 377 TCGA T^CGA
627 TCGA
TspRI 612 CACTG CA[GC]TG[ACGT][ACGT]^
928 CAGTG
TthHB8I 377 TCGA T^CGA
627 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AseI AsnI AvaII BanI BbvI
BfaI BmyI BpmI BsaJI BseRI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HhaI HinP1I HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MvaI NdeII NlaIII NlaIV PshBI
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I XhoII