Restriction map for
pFN21AE1646
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# NO CUT ENZYMES ( 182 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BseAI BsgI
BsiEI BsiWI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstF5I BstUI BstXI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DdeI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT22I FbaI FokI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeII MamI
MfeI MflI MluI MroI MseI
MspA1I MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tru9I Tsp509I Tth111I
TthHB8I XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 157 GTCTAC GT^[AC][GT]AC
AluI 121 AGCT AG^CT
AlwI 3 GGATC GGATC(4/5)
Asp700I 66 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 303 CCTAGG C^CTAGG
BalI 35 TGGCCA TGG^CCA
BfaI 304 CTAG C^TAG
BlnI 303 CCTAGG C^CTAGG
BsaWI 133 ACCGGA [AT]^CCGG[AT]
BseRI 117 GAGGAG GAGGAG(10/8)
BsrBI 151 GAGCGG CCGCTC(-3/-3)
BsrDI 20 GCAATG GCAATG(2/0)
BssKI 328 CCTGG ^CC[ACGT]GG
BstEII 298 GGTTACC G^GT[ACGT]ACC
BstNI 328 CCTGG CC^[AT]GG
BstPI 298 GGTTACC G^GT[ACGT]ACC
Cac8I 382 GCTGGC GC[ACGT]^[ACGT]GC
DraII 103 AGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 35 TGGCCA [CT]^GGCC[GA]
EarI 358 CTCTTC CTCTTC(1/4)
EcoO109I 103 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 298 GGTTACC G^GT[ACGT]ACC
EcoRII 328 CCTGG ^CC[AT]GG
EcoT14I 303 CCTAGG C^C[AT][AT]GG
HaeIII 36 GGCC GG^CC
HapII 134 CCGG C^CGG
HinfI 71 GATTC G^A[ACGT]TC
HpaII 134 CCGG C^CGG
HphI 44 TCACC GGTGA(8/7)
Ksp632I 358 CTCTTC CTCTTC(1/4)
MaeI 304 CTAG C^TAG
MluNI 35 TGGCCA TGG^CCA
MscI 35 TGGCCA TGG^CCA
MslI 51 CATCAGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 134 CCGG C^CGG
MvaI 328 CCTGG CC^[AT]GG
NlaIII 372 CATG CATG^
NlaIV 104 GGACCC GG[ACGT]^[ACGT]CC
PflMI 81 CCATAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 103 AGGACCC [GA]G^G[AT]CC[CT]
ScaI 287 AGTACT AGT^ACT
ScrFI 328 CCTGG CC^[ACGT]GG
SfcI 14 CTGTAG C^T[GA][CT]AG
StyI 303 CCTAGG C^C[AT][AT]GG
TfiI 71 GATTC G^A[AT]TC
TspRI 162 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 81 CCATAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 66 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
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AciI 153 GCGG CCGC(-3/-1)
220 GCGG
BbvI 233 GCAGC GCAGC(8/12)
247 GCTGC
BsaJI 303 CCTAGG C^C[ACGT][ACGT]GG
327 CCCTGG
DpnII 4 GATC ^GATC
447 GATC
FauI 153 GCGGG CCCGC(4/6)
220 GCGGG
Fnu4HI 233 GCAGC GC^[ACGT]GC
247 GCTGC
ItaI 233 GCAGC GC^[ACGT]GC
247 GCTGC
MboI 4 GATC ^GATC
447 GATC
MboII 359 TCTTC GAAGA(8/7)
407 TCTTC
MwoI 247 GCTGCGTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
323 GCACCCCTGGC
NdeII 4 GATC ^GATC
447 GATC
Sau3AI 4 GATC ^GATC
447 GATC
SfaNI 184 GATGC GCATC(5/9)
350 GCATC
TseI 233 GCAGC G^C[AT]GC
247 GCTGC
Tsp45I 43 GTCAC ^GT[GC]AC
422 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 13 enzymes) #
#########################################
AfaI AspHI AvaII BmyI BsiHKAI
BsiYI BslI Bsp1286I Cfr13I MaeIII
MnlI RsaI Sau96I