Restriction map for
pFN21AE5824
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# NO CUT ENZYMES ( 133 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
AocI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BcgI BclI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaHI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmI BsoBI
BspDI BspEI BspLU11I BspMI BsrDI
BsrFI BssHII BssSI Bst1107I BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI FbaI FseI
FspI HaeII HapII HhaI Hin1I
HinP1I HpaI HpaII KasI KpnI
KspI MluI MluNI MroI MscI
MspI MvnI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I StuI SwaI Tth111I XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AflIII 2148 ACGTGT A^C[GA][CT]GT
Alw44I 736 GTGCAC G^TGCAC
ApaLI 736 GTGCAC G^TGCAC
BpmI 640 CTGGAG CTGGAG(16/14)
BsaAI 2428 CACGTA [CT]AC^GT[GA]
BspHI 2901 TCATGA T^CATGA
BsrBI 508 GAGCGG CCGCTC(-3/-3)
BstAPI 3208 GCATTAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2744 TTCGAA TT^CGAA
BstEII 1128 GGTCACC G^GT[ACGT]ACC
BstPI 1128 GGTCACC G^GT[ACGT]ACC
DraI 2395 TTTAAA TTT^AAA
DraII 2490 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 2490 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1128 GGTCACC G^GT[ACGT]ACC
EcoRV 63 GATATC GAT^ATC
EcoT14I 503 CCTTGG C^C[AT][AT]GG
EcoT22I 1301 ATGCAT ATGCA^T
FauI 2727 CCCGC CCCGC(4/6)
HgaI 2095 GACGC GACGC(5/10)
NdeI 1114 CATATG CA^TATG
NsiI 1301 ATGCAT ATGCA^T
NspV 2744 TTCGAA TT^CGAA
PstI 223 CTGCAG CTGCA^G
RcaI 2901 TCATGA T^CATGA
SacI 2579 GAGCTC GAGCT^C
SfuI 2744 TTCGAA TT^CGAA
SphI 2857 GCATGC GCATG^C
StyI 503 CCTTGG C^C[AT][AT]GG
XbaI 2757 TCTAGA T^CTAGA
XcmI 2592 CCACAGTTCTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AlwI 559 GATCC GGATC(4/5)
219 GGATC
AlwNI 47 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
2233 CAGTCTCTG
BanI 1157 GGCACC G^G[CT][GA]CC
2766 GGTGCC
BanII 243 GGGCTC G[GA]GC[CT]^C
2579 GAGCTC
BbvCI 1619 CCTCAGC CCTCAGC(-5/-2)
3203 CCTCAGC
BciVI 719 GTATCC GTATCC(6/5)
2431 GTATCC
BsaBI 614 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3253 GATGAATATC
BsaI 913 GAGACC GGTCTC(1/5)
2780 GGTCTC
BseRI 2465 CTCCTC GAGGAG(10/8)
3201 CTCCTC
BsmFI 49 GGGAC GGGAC(10/14)
1070 GGGAC
BstYI 219 GGATCT [GA]^GATC[CT]
558 AGATCC
DraIII 76 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
2315 CACTCTGTG
EcoRI 1853 GAATTC G^AATTC
3028 GAATTC
HindIII 1811 AAGCTT A^AGCTT
1927 AAGCTT
MaeII 2148 ACGT A^CGT
2429 ACGT
MamI 614 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3253 GATGAATATC
MflI 219 GGATCT [GA]^GATC[CT]
558 AGATCC
NlaIV 1157 GGCACC GG[ACGT]^[ACGT]CC
2766 GGTGCC
SapI 318 GAAGAGC GCTCTTC(1/4)
1047 GCTCTTC
SspI 897 AATATT AAT^ATT
1744 AATATT
XhoII 219 GGATCT [GA]^GATC[CT]
558 AGATCC
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# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AspHI AvaII BbsI BbvI BfaI
BmyI BpuAI BsaJI BsiHKAI BsiYI
BslI BsmAI Bsp1286I Bsp1407I BsrGI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EarI EcoRII
Fnu4HI FokI HaeIII HincII HindII
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MfeI MnlI
MseI MslI MspA1I MunI MvaI
MwoI NdeII NlaIII NspI PflMI
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspBI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I