Restriction map for
pFN21AE5326
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# NO CUT ENZYMES ( 175 enzymes) #
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AatII Acc65I AccII AccIII AcsI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI BmyI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspLU11I
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I Cac8I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HpaII HphI KasI KpnI
Ksp632I KspI MamI MfeI MluI
MluNI MroI MscI MspI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NlaIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
TaqI Tsp45I Tth111I TthHB8I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 595 GTAGAC GT^[AC][GT]AC
AclI 229 AACGTT AA^CGTT
AfaI 167 GTAC GT^AC
AflIII 599 ACGTGT A^C[GA][CT]GT
AlwI 405 GATCC GGATC(4/5)
AvaII 460 GGACC G^G[AT]CC
BbvCI 426 GCTGAGG CCTCAGC(-5/-2)
BbvI 4 GCTGC GCAGC(8/12)
BsmFI 111 GTCCC GGGAC(10/14)
BspHI 389 TCATGA T^CATGA
BsrBI 415 GAGCGG CCGCTC(-3/-3)
BsrDI 281 GCAATG GCAATG(2/0)
BstYI 404 AGATCC [GA]^GATC[CT]
Cfr13I 460 GGACC G^G[ACGT]CC
DpnII 405 GATC ^GATC
DsaI 798 CCATGG C^C[GA][CT]GG
EcoNI 468 CCTGAGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 798 CCATGG C^C[AT][AT]GG
HaeIII 677 GGCC GG^CC
HincII 24 GTTAAC GT[CT]^[GA]AC
HindII 24 GTTAAC GT[CT]^[GA]AC
HindIII 701 AAGCTT A^AGCTT
HpaI 24 GTTAAC GTT^AAC
MaeIII 227 GTAAC ^GT[ACGT]AC
MboI 405 GATC ^GATC
MflI 404 AGATCC [GA]^GATC[CT]
MslI 799 CATGGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 170 CCGCTG C[AC]G^C[GT]G
NcoI 798 CCATGG C^CATGG
NdeII 405 GATC ^GATC
NspI 296 ACATGC [GA]CATG^[CT]
PleI 134 GACTC GAGTC(4/5)
Psp1406I 229 AACGTT AA^CGTT
PstI 374 CTGCAG CTGCA^G
RcaI 389 TCATGA T^CATGA
RsaI 167 GTAC GT^AC
Sau3AI 405 GATC ^GATC
Sau96I 460 GGACC G^G[ACGT]CC
SfaNI 828 GCATC GCATC(5/9)
SfcI 374 CTGCAG C^T[GA][CT]AG
SmlI 761 CTCAAG C^T[CT][GA]AG
SspI 496 AATATT AAT^ATT
StyI 798 CCATGG C^C[AT][AT]GG
TseI 4 GCTGC G^C[AT]GC
TspRI 541 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 642 CCAGACTATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 404 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
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AciI 170 CCGC CCGC(-3/-1)
417 GCGG
AlwNI 368 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
421 CAGAGGCTG
BbsI 175 GAAGAC GAAGAC(2/6)
489 GTCTTC
BfaI 562 CTAG C^TAG
674 CTAG
BpmI 409 CTCCAG CTGGAG(16/14)
640 CTCCAG
BpuAI 175 GAAGAC GAAGAC(2/6)
489 GTCTTC
BsaI 335 GAGACC GGTCTC(1/5)
766 GAGACC
BsaJI 770 CCCAGG C^C[ACGT][ACGT]GG
798 CCATGG
BseRI 619 GAGGAG GAGGAG(10/8)
739 GAGGAG
BspMI 338 ACCTGC ACCTGC(4/8)
372 ACCTGC
BsrI 345 ACTGG ACTGG(1/-1)
457 ACTGG
BstF5I 81 CATCC GGATG(2/0)
327 GGATG
DdeI 427 CTGAG C^T[ACGT]AG
469 CTGAG
Fnu4HI 4 GCTGC GC^[ACGT]GC
417 GCGGC
FokI 81 CATCC GGATG(9/13)
327 GGATG
ItaI 4 GCTGC GC^[ACGT]GC
417 GCGGC
MaeI 562 CTAG C^TAG
674 CTAG
MaeII 230 ACGT A^CGT
599 ACGT
MwoI 333 GCGAGACCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
417 GCGGCAGAGGC
TfiI 534 GATTC G^A[AT]TC
814 GAATC
Tsp509I 147 AATT ^AATT
243 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AluI BsiYI BslI BsmAI BssKI
BstNI EcoRII HinfI MboII MnlI
MseI MvaI NlaIII ScrFI Tru9I