Restriction map for
pFN21AE4033
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccI AclI AfaI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BalI
BamHI BanII BbrPI BbsI BbvCI
BcgI BclI BfrI BglII BlnI
BpmI BpuAI BsaAI BsaBI BsaI
BsiHKAI BsiWI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI DsaI Eam1105I Eco47III Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI HphI KpnI KspI MaeII
MaeIII MamI MfeI MluI MluNI
MscI MseI MspA1I MunI NcoI
NdeI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SmlI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Tru9I Tsp45I Tth111I
Van91I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 109 CGCG CG^CG
AccIII 392 TCCGGA T^CCGGA
AcsI 524 AAATTT [GA]^AATT[CT]
ApoI 524 AAATTT [GA]^AATT[CT]
BanI 100 GGCGCC G^G[CT][GA]CC
BbeI 100 GGCGCC GGCGC^C
BbvI 509 GCAGC GCAGC(8/12)
BciVI 240 GGATAC GTATCC(6/5)
BglI 107 GCCGCGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 9 GCTCAGC GC^T[ACGT]AGC
BmyI 22 GGGCAC G[AGT]GC[ACT]^C
Bpu1102I 9 GCTCAGC GC^T[ACGT]AGC
BsaWI 392 TCCGGA [AT]^CCGG[AT]
BseAI 392 TCCGGA T^CCGGA
BseRI 54 CTCCTC GAGGAG(10/8)
BsmBI 672 GAGACG CGTCTC(1/5)
Bsp1286I 22 GGGCAC G[AGT]GC[ACT]^C
BspEI 392 TCCGGA T^CCGGA
BspMI 465 GCAGGT ACCTGC(4/8)
BsrDI 124 GCAATG GCAATG(2/0)
BsrFI 103 GCCGGC [GA]^CCGG[CT]
BstAPI 24 GCACTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 109 CGCG CG^CG
BstXI 497 CCAATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 9 GCTCAGC GC^T[ACGT]AGC
Cfr10I 103 GCCGGC [GA]^CCGG[CT]
EcoNI 273 CCTCCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 74 CCAAGG C^C[AT][AT]GG
FauI 644 CCCGC CCCGC(4/6)
HaeII 100 GGCGCC [GA]GCGC^[CT]
KasI 100 GGCGCC G^GCGCC
MroI 392 TCCGGA T^CCGGA
MslI 426 CATCATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 109 CGCG CG^CG
NaeI 103 GCCGGC GCC^GGC
NarI 100 GGCGCC GG^CGCC
NgoMIV 103 GCCGGC G^CCGGC
NheI 492 GCTAGC G^CTAGC
NotI 3 GCGGCCGC GC^GGCCGC
PstI 229 CTGCAG CTGCA^G
SapI 620 GAAGAGC GCTCTTC(1/4)
SfcI 229 CTGCAG C^T[GA][CT]AG
SfoI 100 GGCGCC GGC^GCC
SmaI 247 CCCGGG CCC^GGG
SphI 456 GCATGC GCATG^C
StyI 74 CCAAGG C^C[AT][AT]GG
TseI 509 GCAGC G^C[AT]GC
Tsp509I 525 AATT ^AATT
XmaCI 247 CCCGGG C^CCGGG
XmaI 247 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcyI 100 GGCGCC G[GA]^CG[CT]C
253 GGCGTC
AlwI 234 GATCC GGATC(4/5)
261 GATCC
AlwNI 661 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
685 CAGGCTCTG
AvaI 46 CCCGAG C^[CT]CG[GA]G
247 CCCGGG
BfaI 493 CTAG C^TAG
533 CTAG
BsaHI 100 GGCGCC G[GA]^CG[CT]C
253 GGCGTC
BsiEI 4 CGGCCG CG[GA][CT]^CG
105 CGGCCG
BsmFI 215 GGGAC GGGAC(10/14)
446 GGGAC
BsoBI 46 CCCGAG C^[CT]CG[GA]G
247 CCCGGG
BsrBI 7 CCGCTC CCGCTC(-3/-3)
645 CCGCTC
BsrI 87 ACTGG ACTGG(1/-1)
720 ACTGG
BstNI 237 CCAGG CC^[AT]GG
279 CCAGG
BstYI 233 AGATCC [GA]^GATC[CT]
260 AGATCC
DdeI 10 CTCAG C^T[ACGT]AG
604 CTCAG
DraII 446 GGGACCT [GA]G^G[ACGT]CC[CT]
662 AGGACCT
EaeI 4 CGGCCG [CT]^GGCC[GA]
105 CGGCCG
EagI 4 CGGCCG C^GGCCG
105 CGGCCG
EarI 620 GAAGAG CTCTTC(1/4)
669 GAAGAG
EclXI 4 CGGCCG C^GGCCG
105 CGGCCG
Eco52I 4 CGGCCG C^GGCCG
105 CGGCCG
EcoO109I 446 GGGACCT [GA]G^G[ACGT]CC[CT]
662 AGGACCT
EcoRII 237 CCAGG ^CC[AT]GG
279 CCAGG
HgaI 674 GACGC GACGC(5/10)
254 GCGTC
Hin1I 100 GGCGCC G[GA]^CG[CT]C
253 GGCGTC
Ksp632I 620 GAAGAG CTCTTC(1/4)
669 GAAGAG
MaeI 493 CTAG C^TAG
533 CTAG
MflI 233 AGATCC [GA]^GATC[CT]
260 AGATCC
MvaI 237 CCAGG CC^[AT]GG
279 CCAGG
PleI 409 GACTC GAGTC(4/5)
49 GAGTC
PpuMI 446 GGGACCT [GA]G^G[AT]CC[CT]
662 AGGACCT
SfaNI 594 GATGC GCATC(5/9)
570 GCATC
TaqI 209 TCGA T^CGA
287 TCGA
TfiI 152 GATTC G^A[AT]TC
289 GATTC
TthHB8I 209 TCGA T^CGA
287 TCGA
XhoII 233 AGATCC [GA]^GATC[CT]
260 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AluI AvaII BcnI BsaJI
BsgI BsiYI BslI BsmAI BssKI
BstF5I Cac8I CfoI Cfr13I DpnII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MboI
MboII MnlI MspI MwoI NciI
NdeII NlaIII NlaIV NspI Sau3AI
Sau96I ScrFI TspRI