################################## # NO CUT ENZYMES ( 135 enzymes) # ################################## AatII Acc65I AccI AclI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanII BbrPI BbsI BbvCI BcgI BclI BfrI BglII BlnI BpmI BpuAI BsaAI BsaBI BsaI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI DsaI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI HphI KpnI KspI MaeII MaeIII MamI MfeI MluI MluNI MscI MseI MspA1I MunI NcoI NdeI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp45I Tth111I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccII 109 CGCG CG^CG AccIII 392 TCCGGA T^CCGGA AcsI 524 AAATTT [GA]^AATT[CT] ApoI 524 AAATTT [GA]^AATT[CT] BanI 100 GGCGCC G^G[CT][GA]CC BbeI 100 GGCGCC GGCGC^C BbvI 509 GCAGC GCAGC(8/12) BciVI 240 GGATAC GTATCC(6/5) BglI 107 GCCGCGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 9 GCTCAGC GC^T[ACGT]AGC BmyI 22 GGGCAC G[AGT]GC[ACT]^C Bpu1102I 9 GCTCAGC GC^T[ACGT]AGC BsaWI 392 TCCGGA [AT]^CCGG[AT] BseAI 392 TCCGGA T^CCGGA BseRI 54 CTCCTC GAGGAG(10/8) BsmBI 672 GAGACG CGTCTC(1/5) Bsp1286I 22 GGGCAC G[AGT]GC[ACT]^C BspEI 392 TCCGGA T^CCGGA BspMI 465 GCAGGT ACCTGC(4/8) BsrDI 124 GCAATG GCAATG(2/0) BsrFI 103 GCCGGC [GA]^CCGG[CT] BstAPI 24 GCACTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 109 CGCG CG^CG BstXI 497 CCAATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 9 GCTCAGC GC^T[ACGT]AGC Cfr10I 103 GCCGGC [GA]^CCGG[CT] EcoNI 273 CCTCCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 74 CCAAGG C^C[AT][AT]GG FauI 644 CCCGC CCCGC(4/6) HaeII 100 GGCGCC [GA]GCGC^[CT] KasI 100 GGCGCC G^GCGCC MroI 392 TCCGGA T^CCGGA MslI 426 CATCATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 109 CGCG CG^CG NaeI 103 GCCGGC GCC^GGC NarI 100 GGCGCC GG^CGCC NgoMIV 103 GCCGGC G^CCGGC NheI 492 GCTAGC G^CTAGC NotI 3 GCGGCCGC GC^GGCCGC PstI 229 CTGCAG CTGCA^G SapI 620 GAAGAGC GCTCTTC(1/4) SfcI 229 CTGCAG C^T[GA][CT]AG SfoI 100 GGCGCC GGC^GCC SmaI 247 CCCGGG CCC^GGG SphI 456 GCATGC GCATG^C StyI 74 CCAAGG C^C[AT][AT]GG TseI 509 GCAGC G^C[AT]GC Tsp509I 525 AATT ^AATT XmaCI 247 CCCGGG C^CCGGG XmaI 247 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AcyI 100 GGCGCC G[GA]^CG[CT]C 253 GGCGTC AlwI 234 GATCC GGATC(4/5) 261 GATCC AlwNI 661 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG 685 CAGGCTCTG AvaI 46 CCCGAG C^[CT]CG[GA]G 247 CCCGGG BfaI 493 CTAG C^TAG 533 CTAG BsaHI 100 GGCGCC G[GA]^CG[CT]C 253 GGCGTC BsiEI 4 CGGCCG CG[GA][CT]^CG 105 CGGCCG BsmFI 215 GGGAC GGGAC(10/14) 446 GGGAC BsoBI 46 CCCGAG C^[CT]CG[GA]G 247 CCCGGG BsrBI 7 CCGCTC CCGCTC(-3/-3) 645 CCGCTC BsrI 87 ACTGG ACTGG(1/-1) 720 ACTGG BstNI 237 CCAGG CC^[AT]GG 279 CCAGG BstYI 233 AGATCC [GA]^GATC[CT] 260 AGATCC DdeI 10 CTCAG C^T[ACGT]AG 604 CTCAG DraII 446 GGGACCT [GA]G^G[ACGT]CC[CT] 662 AGGACCT EaeI 4 CGGCCG [CT]^GGCC[GA] 105 CGGCCG EagI 4 CGGCCG C^GGCCG 105 CGGCCG EarI 620 GAAGAG CTCTTC(1/4) 669 GAAGAG EclXI 4 CGGCCG C^GGCCG 105 CGGCCG Eco52I 4 CGGCCG C^GGCCG 105 CGGCCG EcoO109I 446 GGGACCT [GA]G^G[ACGT]CC[CT] 662 AGGACCT EcoRII 237 CCAGG ^CC[AT]GG 279 CCAGG HgaI 674 GACGC GACGC(5/10) 254 GCGTC Hin1I 100 GGCGCC G[GA]^CG[CT]C 253 GGCGTC Ksp632I 620 GAAGAG CTCTTC(1/4) 669 GAAGAG MaeI 493 CTAG C^TAG 533 CTAG MflI 233 AGATCC [GA]^GATC[CT] 260 AGATCC MvaI 237 CCAGG CC^[AT]GG 279 CCAGG PleI 409 GACTC GAGTC(4/5) 49 GAGTC PpuMI 446 GGGACCT [GA]G^G[AT]CC[CT] 662 AGGACCT SfaNI 594 GATGC GCATC(5/9) 570 GCATC TaqI 209 TCGA T^CGA 287 TCGA TfiI 152 GATTC G^A[AT]TC 289 GATTC TthHB8I 209 TCGA T^CGA 287 TCGA XhoII 233 AGATCC [GA]^GATC[CT] 260 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AciI AluI AvaII BcnI BsaJI BsgI BsiYI BslI BsmAI BssKI BstF5I Cac8I CfoI Cfr13I DpnII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MboI MboII MnlI MspI MwoI NciI NdeII NlaIII NlaIV NspI Sau3AI Sau96I ScrFI TspRI