Restriction map for
pFN21AE9402
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# NO CUT ENZYMES ( 142 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DsaI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HgaI Hin1I HindIII HpaI
KasI KpnI KspI MaeI MamI
MfeI MluI MroI MslI MunI
NarI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI TfiI TthHB8I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AlwI 921 GATCC GGATC(4/5)
AlwNI 113 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 930 AGCGCT AGC^GCT
AspI 142 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 713 TGGCCA TGG^CCA
BanII 121 GAGCTC G[GA]GC[CT]^C
BbsI 7 GAAGAC GAAGAC(2/6)
BglII 656 AGATCT A^GATCT
BpuAI 7 GAAGAC GAAGAC(2/6)
BsaI 747 GGTCTC GGTCTC(1/5)
BseRI 87 CTCCTC GAGGAG(10/8)
BsiEI 347 CGATCG CG[GA][CT]^CG
BsmBI 548 GAGACG CGTCTC(1/5)
BsrBI 577 CCGCTC CCGCTC(-3/-3)
BsrFI 277 GCCGGC [GA]^CCGG[CT]
BssHII 982 GCGCGC G^CGCGC
Cfr10I 277 GCCGGC [GA]^CCGG[CT]
DdeI 119 CTGAG C^T[ACGT]AG
DraII 496 GGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 724 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 10 GACCTGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 619 CTCTTC CTCTTC(1/4)
Eco47III 930 AGCGCT AGC^GCT
EcoO109I 496 GGGACCT [GA]G^G[ACGT]CC[CT]
HaeII 930 AGCGCT [GA]GCGC^[CT]
Ksp632I 619 CTCTTC CTCTTC(1/4)
MluNI 713 TGGCCA TGG^CCA
MscI 713 TGGCCA TGG^CCA
MseI 25 TTAA T^TAA
MspA1I 56 CCGCTG C[AC]G^C[GT]G
NaeI 277 GCCGGC GCC^GGC
NgoMIV 277 GCCGGC G^CCGGC
PflFI 142 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 705 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 496 GGGACCT [GA]G^G[AT]CC[CT]
PvuI 347 CGATCG CGAT^CG
SacI 121 GAGCTC GAGCT^C
SmaI 92 CCCGGG CCC^GGG
Tru9I 25 TTAA T^TAA
Tth111I 142 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 705 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 557 CCACCTGCAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 92 CCCGGG C^CCGGG
XmaI 92 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccII 594 CGCG CG^CG
983 CGCG
AspHI 121 GAGCTC G[AT]GC[AT]^C
227 GAGCAC
AvaII 195 GGTCC G^G[AT]CC
497 GGACC
BmyI 121 GAGCTC G[AGT]GC[ACT]^C
227 GAGCAC
BsiHKAI 121 GAGCTC G[AT]GC[AT]^C
227 GAGCAC
Bsp1286I 121 GAGCTC G[AGT]GC[ACT]^C
227 GAGCAC
BsrI 965 CCAGT ACTGG(1/-1)
992 CCAGT
BstUI 594 CGCG CG^CG
983 CGCG
BstXI 332 CCACCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
503 CCATCTGCCTGG
BstYI 656 AGATCT [GA]^GATC[CT]
920 AGATCC
Cfr13I 195 GGTCC G^G[ACGT]CC
497 GGACC
FauI 865 CCCGC CCCGC(4/6)
29 GCGGG
HincII 130 GTCAAC GT[CT]^[GA]AC
406 GTTGAC
HindII 130 GTCAAC GT[CT]^[GA]AC
406 GTTGAC
HinfI 287 GACTC G^A[ACGT]TC
553 GAGTC
MflI 656 AGATCT [GA]^GATC[CT]
920 AGATCC
MvnI 594 CGCG CG^CG
983 CGCG
PleI 287 GACTC GAGTC(4/5)
553 GAGTC
Sau96I 195 GGTCC G^G[ACGT]CC
497 GGACC
XhoII 656 AGATCT [GA]^GATC[CT]
920 AGATCC
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# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AfaI AluI AvaI BbvI
BcnI BsaJI BsiYI BslI BsmAI
BsmFI BsoBI BspMI BssKI BstF5I
BstNI Cac8I CfoI DpnII EaeI
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PstI RsaI Sau3AI
ScrFI SfaNI SfcI TseI Tsp45I
Tsp509I TspRI