Restriction map for
pFN21AE1703
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# NO CUT ENZYMES ( 163 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI AspHI AspI AviII
AvrII BalI BamHI BbeI BbsI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsmAI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Cac8I CelII CfoI ClaI
CpoI DraIII DrdI DsaI EaeI
EagI EarI EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI Ksp632I
KspI MamI MfeI MflI MluI
MluNI MroI MscI MslI MspA1I
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NlaIII NotI
NruI NsiI NspI PacI PaeR7I
PflFI PflMI PinAI PmeI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SmlI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tth111I Van91I XbaI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Acc65I 48 GGTACC G^GTACC
AhdI 143 GACCCGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 8 CCTTAGG CC^T[ACGT]AGG
Asp700I 24 GAACTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 48 GGTACC G^GTACC
AspEI 143 GACCCGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII 130 GGGCTC G[GA]GC[CT]^C
BbrPI 463 CACGTG CAC^GTG
BbvI 192 GCAGC GCAGC(8/12)
BcnI 87 CCCGG CC^[GC]GG
BglI 269 GCCGATTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI 37 GTGCAG GTGCAG(16/14)
BsrFI 75 GCCGGT [GA]^CCGG[CT]
BstBI 418 TTCGAA TT^CGAA
Bsu36I 8 CCTTAGG CC^T[ACGT]AGG
Cfr10I 75 GCCGGT [GA]^CCGG[CT]
DraI 168 TTTAAA TTT^AAA
Eam1105I 143 GACCCGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 8 CCTTAGG CC^T[ACGT]AGG
FauI 145 CCCGC CCCGC(4/6)
Fnu4HI 192 GCAGC GC^[ACGT]GC
HaeIII 307 GGCC GG^CC
HphI 13 GGTGA GGTGA(8/7)
ItaI 192 GCAGC GC^[ACGT]GC
KpnI 48 GGTACC GGTAC^C
NciI 87 CCCGG CC^[GC]GG
NspV 418 TTCGAA TT^CGAA
PleI 338 GACTC GAGTC(4/5)
PmaCI 463 CACGTG CAC^GTG
PmlI 463 CACGTG CAC^GTG
PstI 201 CTGCAG CTGCA^G
SfaNI 43 GATGC GCATC(5/9)
SfcI 201 CTGCAG C^T[GA][CT]AG
SfuI 418 TTCGAA TT^CGAA
SnaBI 438 TACGTA TAC^GTA
TfiI 177 GATTC G^A[AT]TC
TseI 192 GCAGC G^C[AT]GC
XcmI 264 CCACAGCCGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 24 GAACTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcsI 286 GAATTT [GA]^AATT[CT]
547 AAATTC
AluI 194 AGCT AG^CT
368 AGCT
AlwI 162 GGATC GGATC(4/5)
376 GGATC
ApoI 286 GAATTT [GA]^AATT[CT]
547 AAATTC
AvaI 346 CTCGGG C^[CT]CG[GA]G
513 CCCGAG
BanI 48 GGTACC G^G[CT][GA]CC
539 GGCACC
BfaI 527 CTAG C^TAG
566 CTAG
BmyI 64 GTGCCC G[AGT]GC[ACT]^C
130 GGGCTC
BsaAI 438 TACGTA [CT]AC^GT[GA]
463 CACGTG
BsiYI 119 CCGAAGCAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
514 CCGAGAGAAGG
BslI 119 CCGAAGCAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
514 CCGAGAGAAGG
BsmI 278 GCATTC GAATGC(1/-1)
317 GCATTC
BsoBI 346 CTCGGG C^[CT]CG[GA]G
513 CCCGAG
Bsp1286I 64 GTGCCC G[AGT]GC[ACT]^C
130 GGGCTC
BstF5I 42 GGATG GGATG(2/0)
350 GGATG
DpnII 163 GATC ^GATC
377 GATC
DraII 141 GGGACCC [GA]G^G[ACGT]CC[CT]
508 GGGACCC
EcoO109I 141 GGGACCC [GA]G^G[ACGT]CC[CT]
508 GGGACCC
FokI 42 GGATG GGATG(9/13)
350 GGATG
HapII 76 CCGG C^CGG
88 CCGG
HinfI 177 GATTC G^A[ACGT]TC
338 GACTC
HpaII 76 CCGG C^CGG
88 CCGG
MaeI 527 CTAG C^TAG
566 CTAG
MaeII 439 ACGT A^CGT
464 ACGT
MaeIII 101 GTGAC ^GT[ACGT]AC
151 GTCAC
MboI 163 GATC ^GATC
377 GATC
MboII 421 GAAGA GAAGA(8/7)
227 TCTTC
MspI 76 CCGG C^CGG
88 CCGG
NdeII 163 GATC ^GATC
377 GATC
PpuMI 141 GGGACCC [GA]G^G[AT]CC[CT]
508 GGGACCC
Sau3AI 163 GATC ^GATC
377 GATC
TaqI 321 TCGA T^CGA
419 TCGA
Tsp45I 101 GTGAC ^GT[GC]AC
151 GTCAC
Tsp509I 287 AATT ^AATT
548 AATT
TthHB8I 321 TCGA T^CGA
419 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AciI AfaI AvaII BsaJI BsmFI
BsrI BssKI BstNI Cfr13I DdeI
EcoRII MnlI MseI MvaI MwoI
NlaIV RsaI Sau96I ScrFI Tru9I
TspRI