Restriction map for
pFN21AE3368
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# NO CUT ENZYMES ( 79 enzymes) #
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AatII AccI AclI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp700I AspEI
AspI AvrII BamHI BciVI BfrI
BlnI BsaBI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrDI BsrGI BssSI
Bst1107I BstEII BstPI BstZ17I ClaI
DraI DrdI Eam1105I Eco47III EcoO65I
EcoRV EcoT22I FseI MamI MfeI
MluI MslI MunI NdeI NheI
NruI NsiI PacI PaeR7I PflFI
PinAI PmeI PshBI Psp1406I PvuI
RcaI SalI SbfI ScaI SexAI
SfiI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SwaI Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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Acc65I 562 GGTACC G^GTACC
Asp718I 562 GGTACC G^GTACC
BbvCI 1347 GCTGAGG CCTCAGC(-5/-2)
BclI 2774 TGATCA T^GATCA
BfaI 2569 CTAG C^TAG
BglI 1117 GCCACCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2397 AGATCT A^GATCT
BsaI 844 GAGACC GGTCTC(1/5)
BsiWI 629 CGTACG C^GTACG
BsmBI 1754 GAGACG CGTCTC(1/5)
BsrBI 558 GAGCGG CCGCTC(-3/-3)
BstBI 517 TTCGAA TT^CGAA
CpoI 1248 CGGTCCG CG^G[AT]CCG
DraIII 1324 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 3115 GAATTC G^AATTC
FbaI 2774 TGATCA T^GATCA
HincII 1861 GTTAAC GT[CT]^[GA]AC
HindII 1861 GTTAAC GT[CT]^[GA]AC
HpaI 1861 GTTAAC GTT^AAC
KpnI 562 GGTACC GGTAC^C
MaeI 2569 CTAG C^TAG
NcoI 2987 CCATGG C^CATGG
NotI 762 GCGGCCGC GC^GGCCGC
NspV 517 TTCGAA TT^CGAA
PpuMI 2834 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 1296 GACGGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 1248 CGGTCCG CG^G[AT]CCG
SapI 1332 GAAGAGC GCTCTTC(1/4)
SfuI 517 TTCGAA TT^CGAA
SphI 1467 GCATGC GCATG^C
SspI 2468 AATATT AAT^ATT
StuI 3059 AGGCCT AGG^CCT
XcmI 339 CCAGGAGATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 28 enzymes) #
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AccIII 2273 TCCGGA T^CCGGA
2945 TCCGGA
AcsI 3000 GAATTT [GA]^AATT[CT]
3115 GAATTC
AocI 2094 CCTAAGG CC^T[ACGT]AGG
2824 CCTGAGG
ApoI 3000 GAATTT [GA]^AATT[CT]
3115 GAATTC
AviII 1571 TGCGCA TGC^GCA
1609 TGCGCA
BalI 1892 TGGCCA TGG^CCA
2330 TGGCCA
BbrPI 1177 CACGTG CAC^GTG
1825 CACGTG
BseAI 2273 TCCGGA T^CCGGA
2945 TCCGGA
BsgI 2436 CTGCAC GTGCAG(16/14)
805 GTGCAG
BspEI 2273 TCCGGA T^CCGGA
2945 TCCGGA
BspMI 770 ACCTGC ACCTGC(4/8)
1562 ACCTGC
BstXI 146 CCAAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
602 CCACGGCGCTGG
BstYI 1937 AGATCC [GA]^GATC[CT]
2397 AGATCT
Bsu36I 2094 CCTAAGG CC^T[ACGT]AGG
2824 CCTGAGG
Eco81I 2094 CCTAAGG CC^T[ACGT]AGG
2824 CCTGAGG
EcoNI 2157 CCTGGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3122 CCTTCCGAAGG
FspI 1571 TGCGCA TGC^GCA
1609 TGCGCA
HindIII 582 AAGCTT A^AGCTT
3185 AAGCTT
MflI 1937 AGATCC [GA]^GATC[CT]
2397 AGATCT
MluNI 1892 TGGCCA TGG^CCA
2330 TGGCCA
MroI 2273 TCCGGA T^CCGGA
2945 TCCGGA
MscI 1892 TGGCCA TGG^CCA
2330 TGGCCA
NspI 984 GCATGT [GA]CATG^[CT]
1467 GCATGC
PmaCI 1177 CACGTG CAC^GTG
1825 CACGTG
PmlI 1177 CACGTG CAC^GTG
1825 CACGTG
SacI 356 GAGCTC GAGCT^C
3201 GAGCTC
SmlI 228 CTTGAG C^T[CT][GA]AG
361 CTCAAG
XhoII 1937 AGATCC [GA]^GATC[CT]
2397 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BanI BanII BbeI BbsI
BbvI BcgI BcnI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaJI BsaWI BseRI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrFI BsrI BssHII BssKI
BstAPI BstF5I BstNI BstUI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EarI EclXI Eco52I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeII MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV PflMI PleI PstI PvuII
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I