################################## # NO CUT ENZYMES ( 43 enzymes) # ################################## AatII Acc65I AclI AflII AgeI AhdI AscI AseI AsnI Asp718I AspEI AvrII BfrI BlnI BsiWI BsmI BssSI Bst1107I BstZ17I CpoI DraI Eam1105I HpaI KpnI MfeI MunI NdeI NheI NruI PacI PinAI PmeI PshBI Psp1406I PvuI RsrII SalI ScaI SgrAI SnaBI SpeI SwaI XbaI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AspI 6652 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BciVI 4448 GTATCC GTATCC(6/5) BspDI 898 ATCGAT AT^CGAT BssHII 27 GCGCGC G^CGCGC BstBI 4657 TTCGAA TT^CGAA BstEII 1077 GGTCACC G^GT[ACGT]ACC BstPI 1077 GGTCACC G^GT[ACGT]ACC ClaI 898 ATCGAT AT^CGAT DrdI 3196 GACCAGATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 1077 GGTCACC G^GT[ACGT]ACC EcoRI 4813 GAATTC G^AATTC EcoRV 1663 GATATC GAT^ATC EcoT22I 196 ATGCAT ATGCA^T FseI 4023 GGCCGGCC GGCCGG^CC HindIII 1816 AAGCTT A^AGCTT MluI 554 ACGCGT A^CGCGT NaeI 4024 GCCGGC GCC^GGC NgoMIV 4024 GCCGGC G^CCGGC NotI 1276 GCGGCCGC GC^GGCCGC NsiI 196 ATGCAT ATGCA^T NspV 4657 TTCGAA TT^CGAA PaeR7I 1639 CTCGAG C^TCGAG PflFI 6652 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC SbfI 6645 CCTGCAGG CCTGCA^GG SfiI 1278 GGCCGCCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 4657 TTCGAA TT^CGAA Sse8387I 6645 CCTGCAGG CCTGCA^GG SspI 6768 AATATT AAT^ATT Tth111I 6652 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 1639 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AccIII 3329 TCCGGA T^CCGGA 6773 TCCGGA Alw44I 1443 GTGCAC G^TGCAC 4315 GTGCAC AocI 1221 CCTCAGG CC^T[ACGT]AGG 6460 CCTGAGG ApaLI 1443 GTGCAC G^TGCAC 4315 GTGCAC AviII 2459 TGCGCA TGC^GCA 4553 TGCGCA BbvCI 2365 GCTGAGG CCTCAGC(-5/-2) 6042 GCTGAGG BclI 5306 TGATCA T^GATCA 6140 TGATCA BfaI 1294 CTAG C^TAG 1315 CTAG BglII 506 AGATCT A^GATCT 2948 AGATCT BseAI 3329 TCCGGA T^CCGGA 6773 TCCGGA BsmBI 2224 CGTCTC CGTCTC(1/5) 829 GAGACG BspEI 3329 TCCGGA T^CCGGA 6773 TCCGGA BspLU11I 1328 ACATGT A^CATGT 4214 ACATGT BsrDI 315 CATTGC GCAATG(2/0) 6780 CATTGC BsrFI 1673 GCCGGT [GA]^CCGG[CT] 4024 GCCGGC Bsu36I 1221 CCTCAGG CC^T[ACGT]AGG 6460 CCTGAGG Cfr10I 1673 GCCGGT [GA]^CCGG[CT] 4024 GCCGGC EagI 1277 CGGCCG C^GGCCG 2342 CGGCCG EclXI 1277 CGGCCG C^GGCCG 2342 CGGCCG Eco52I 1277 CGGCCG C^GGCCG 2342 CGGCCG Eco81I 1221 CCTCAGG CC^T[ACGT]AGG 6460 CCTGAGG FbaI 5306 TGATCA T^GATCA 6140 TGATCA FspI 2459 TGCGCA TGC^GCA 4553 TGCGCA HincII 5206 GTCAAC GT[CT]^[GA]AC 5233 GTCAAC HindII 5206 GTCAAC GT[CT]^[GA]AC 5233 GTCAAC KspI 2925 CCGCGG CCGC^GG 6672 CCGCGG MaeI 1294 CTAG C^TAG 1315 CTAG MroI 3329 TCCGGA T^CCGGA 6773 TCCGGA PshAI 1611 GACCTACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 5101 GACAACTGTC SacII 2925 CCGCGG CCGC^GG 6672 CCGCGG SmaI 1121 CCCGGG CCC^GGG 3910 CCCGGG SphI 1145 GCATGC GCATG^C 6692 GCATGC XmaCI 1121 CCCGGG C^CCGGG 3910 CCCGGG XmaI 1121 CCCGGG C^CCGGG 3910 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 151 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI Aor51HI ApaI ApoI Asp700I AspHI AvaI AvaII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvI BcgI BcnI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspHI BspMI BsrBI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco47III EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RcaI RsaI SacI SapI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SfoI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI