Restriction map for
pFN21AB5624
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# NO CUT ENZYMES ( 43 enzymes) #
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AatII Acc65I AclI AflII AgeI
AhdI AscI AseI AsnI Asp718I
AspEI AvrII BfrI BlnI BsiWI
BsmI BssSI Bst1107I BstZ17I CpoI
DraI Eam1105I HpaI KpnI MfeI
MunI NdeI NheI NruI PacI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SalI ScaI SgrAI SnaBI
SpeI SwaI XbaI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AspI 6652 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 4448 GTATCC GTATCC(6/5)
BspDI 898 ATCGAT AT^CGAT
BssHII 27 GCGCGC G^CGCGC
BstBI 4657 TTCGAA TT^CGAA
BstEII 1077 GGTCACC G^GT[ACGT]ACC
BstPI 1077 GGTCACC G^GT[ACGT]ACC
ClaI 898 ATCGAT AT^CGAT
DrdI 3196 GACCAGATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1077 GGTCACC G^GT[ACGT]ACC
EcoRI 4813 GAATTC G^AATTC
EcoRV 1663 GATATC GAT^ATC
EcoT22I 196 ATGCAT ATGCA^T
FseI 4023 GGCCGGCC GGCCGG^CC
HindIII 1816 AAGCTT A^AGCTT
MluI 554 ACGCGT A^CGCGT
NaeI 4024 GCCGGC GCC^GGC
NgoMIV 4024 GCCGGC G^CCGGC
NotI 1276 GCGGCCGC GC^GGCCGC
NsiI 196 ATGCAT ATGCA^T
NspV 4657 TTCGAA TT^CGAA
PaeR7I 1639 CTCGAG C^TCGAG
PflFI 6652 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SbfI 6645 CCTGCAGG CCTGCA^GG
SfiI 1278 GGCCGCCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 4657 TTCGAA TT^CGAA
Sse8387I 6645 CCTGCAGG CCTGCA^GG
SspI 6768 AATATT AAT^ATT
Tth111I 6652 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1639 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 34 enzymes) #
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AccIII 3329 TCCGGA T^CCGGA
6773 TCCGGA
Alw44I 1443 GTGCAC G^TGCAC
4315 GTGCAC
AocI 1221 CCTCAGG CC^T[ACGT]AGG
6460 CCTGAGG
ApaLI 1443 GTGCAC G^TGCAC
4315 GTGCAC
AviII 2459 TGCGCA TGC^GCA
4553 TGCGCA
BbvCI 2365 GCTGAGG CCTCAGC(-5/-2)
6042 GCTGAGG
BclI 5306 TGATCA T^GATCA
6140 TGATCA
BfaI 1294 CTAG C^TAG
1315 CTAG
BglII 506 AGATCT A^GATCT
2948 AGATCT
BseAI 3329 TCCGGA T^CCGGA
6773 TCCGGA
BsmBI 2224 CGTCTC CGTCTC(1/5)
829 GAGACG
BspEI 3329 TCCGGA T^CCGGA
6773 TCCGGA
BspLU11I 1328 ACATGT A^CATGT
4214 ACATGT
BsrDI 315 CATTGC GCAATG(2/0)
6780 CATTGC
BsrFI 1673 GCCGGT [GA]^CCGG[CT]
4024 GCCGGC
Bsu36I 1221 CCTCAGG CC^T[ACGT]AGG
6460 CCTGAGG
Cfr10I 1673 GCCGGT [GA]^CCGG[CT]
4024 GCCGGC
EagI 1277 CGGCCG C^GGCCG
2342 CGGCCG
EclXI 1277 CGGCCG C^GGCCG
2342 CGGCCG
Eco52I 1277 CGGCCG C^GGCCG
2342 CGGCCG
Eco81I 1221 CCTCAGG CC^T[ACGT]AGG
6460 CCTGAGG
FbaI 5306 TGATCA T^GATCA
6140 TGATCA
FspI 2459 TGCGCA TGC^GCA
4553 TGCGCA
HincII 5206 GTCAAC GT[CT]^[GA]AC
5233 GTCAAC
HindII 5206 GTCAAC GT[CT]^[GA]AC
5233 GTCAAC
KspI 2925 CCGCGG CCGC^GG
6672 CCGCGG
MaeI 1294 CTAG C^TAG
1315 CTAG
MroI 3329 TCCGGA T^CCGGA
6773 TCCGGA
PshAI 1611 GACCTACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5101 GACAACTGTC
SacII 2925 CCGCGG CCGC^GG
6672 CCGCGG
SmaI 1121 CCCGGG CCC^GGG
3910 CCCGGG
SphI 1145 GCATGC GCATG^C
6692 GCATGC
XmaCI 1121 CCCGGG C^CCGGG
3910 CCCGGG
XmaI 1121 CCCGGG C^CCGGG
3910 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 151 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
Aor51HI ApaI ApoI Asp700I AspHI
AvaI AvaII BalI BamHI BanI
BanII BbeI BbrPI BbsI BbvI
BcgI BcnI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspHI BspMI BsrBI BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco47III EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeII
MaeIII MamI MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RcaI
RsaI SacI SapI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SfoI
SmlI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmnI