Restriction map for
pFN21AE9427
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# NO CUT ENZYMES ( 79 enzymes) #
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AatII AclI AflII AgeI AhdI
Alw44I AocI ApaI ApaLI AscI
AseI AsnI AspEI AviII AvrII
BbrPI BfrI BglII BlnI BsiEI
BsiWI BsmBI BsmI BspDI BsrDI
BsrFI BssHII Bst1107I BstBI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoRV FseI FspI HindIII
HpaI KspI MluI NaeI NdeI
NgoMIV NheI NotI NruI NspV
PacI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI PvuII RsrII
SacI SacII SapI ScaI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SspI SwaI XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 2308 GGTACC G^GTACC
Aor51HI 1799 AGCGCT AGC^GCT
Asp700I 2508 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2308 GGTACC G^GTACC
AspI 1063 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1873 CTCGAG C^[CT]CG[GA]G
BamHI 158 GGATCC G^GATCC
BbeI 2131 GGCGCC GGCGC^C
BbvCI 912 CCTCAGC CCTCAGC(-5/-2)
BcgI 2186 GCATCCTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1982 TGATCA T^GATCA
BfaI 353 CTAG C^TAG
BlpI 2565 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2565 GCTCAGC GC^T[ACGT]AGC
BsaAI 1475 CACGTA [CT]AC^GT[GA]
BsaBI 2055 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2249 GAGACC GGTCTC(1/5)
BseRI 1128 CTCCTC GAGGAG(10/8)
BsoBI 1873 CTCGAG C^[CT]CG[GA]G
Bsp1407I 440 TGTACA T^GTACA
BspLU11I 2366 ACATGT A^CATGT
BsrBI 694 GAGCGG CCGCTC(-3/-3)
BsrGI 440 TGTACA T^GTACA
BstAPI 2102 GCAACAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2276 CCAAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2565 GCTCAGC GC^T[ACGT]AGC
DraIII 1684 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1799 AGCGCT AGC^GCT
EcoT22I 1345 ATGCAT ATGCA^T
FbaI 1982 TGATCA T^GATCA
KasI 2131 GGCGCC G^GCGCC
KpnI 2308 GGTACC GGTAC^C
MaeI 353 CTAG C^TAG
MamI 2055 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2106 CAATTG C^AATTG
MseI 973 TTAA T^TAA
MunI 2106 CAATTG C^AATTG
NarI 2131 GGCGCC GG^CGCC
NsiI 1345 ATGCAT ATGCA^T
PaeR7I 1873 CTCGAG C^TCGAG
PflFI 1063 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 2389 CTGCAG CTGCA^G
SalI 2605 GTCGAC G^TCGAC
SbfI 2388 CCTGCAGG CCTGCA^GG
SexAI 1685 ACCAGGT A^CC[AT]GGT
SfoI 2131 GGCGCC GGC^GCC
Sse8387I 2388 CCTGCAGG CCTGCA^GG
SspBI 440 TGTACA T^GTACA
TfiI 954 GAATC G^A[AT]TC
Tru9I 973 TTAA T^TAA
Tth111I 1063 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 352 TCTAGA T^CTAGA
XcmI 487 CCAGGCCCCTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1873 CTCGAG C^TCGAG
XmnI 2508 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AcsI 1179 GAATTC [GA]^AATT[CT]
2427 GAATTC
AflIII 1474 ACACGT A^C[GA][CT]GT
2366 ACATGT
ApoI 1179 GAATTC [GA]^AATT[CT]
2427 GAATTC
BanI 2131 GGCGCC G^G[CT][GA]CC
2308 GGTACC
BglI 482 GCCCCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2134 GCCATCTGGGC
BspHI 785 TCATGA T^CATGA
2528 TCATGA
BstEII 1113 GGTCACC G^GT[ACGT]ACC
1755 GGTCACC
BstPI 1113 GGTCACC G^GT[ACGT]ACC
1755 GGTCACC
EcoO65I 1113 GGTCACC G^GT[ACGT]ACC
1755 GGTCACC
EcoRI 1179 GAATTC G^AATTC
2427 GAATTC
PshAI 501 GACTCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2202 GACCAGAGTC
RcaI 785 TCATGA T^CATGA
2528 TCATGA
SfcI 1971 CTACAG C^T[GA][CT]AG
2389 CTGCAG
SmlI 1459 CTCAAG C^T[CT][GA]AG
1873 CTCGAG
SphI 750 GCATGC GCATG^C
1289 GCATGC
StuI 470 AGGCCT AGG^CCT
1238 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccI AccII AccIII AciI AcyI
AfaI AluI AlwI AlwNI AspHI
AvaII BalI BanII BbsI BbvI
BciVI BcnI BmyI BpmI BpuAI
BsaHI BsaJI BsaWI BseAI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspEI BspMI BsrI BssKI
BssSI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MluNI MnlI MroI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII