Restriction map for
pFN21AE2005
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
Acc65I AclI AcsI AflII AgeI
AhdI AlwNI AocI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvrII BamHI BbvCI BciVI
BfaI BfrI BlnI BlpI Bpu1102I
BsaBI BseRI BsiWI BsmBI BsmI
BspDI BspHI BspLU11I BsrDI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DdeI DraI
Eam1105I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI HindIII HpaI
KpnI KspI MaeI MaeIII MamI
MfeI MluI MunI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
ScaI SfuI SnaBI SpeI SphI
SspI StuI SwaI TfiI Tsp45I
Tsp509I Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 1168 GACGTC GACGT^C
AccI 814 GTCTAC GT^[AC][GT]AC
AccIII 1268 TCCGGA T^CCGGA
AflIII 647 ACGTGT A^C[GA][CT]GT
Alw44I 282 GTGCAC G^TGCAC
Aor51HI 671 AGCGCT AGC^GCT
ApaLI 282 GTGCAC G^TGCAC
AviII 1115 TGCGCA TGC^GCA
BbrPI 598 CACGTG CAC^GTG
BcgI 125 GCAAGGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1649 TGATCA T^GATCA
BsaI 294 GGTCTC GGTCTC(1/5)
BsaWI 1268 TCCGGA [AT]^CCGG[AT]
BseAI 1268 TCCGGA T^CCGGA
BsmAI 295 GTCTC GTCTC(1/5)
Bsp1407I 1349 TGTACA T^GTACA
BspEI 1268 TCCGGA T^CCGGA
BsrBI 625 CCGCTC CCGCTC(-3/-3)
BsrGI 1349 TGTACA T^GTACA
DraIII 595 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1578 GACTCTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 508 CGGCCG C^GGCCG
EclXI 508 CGGCCG C^GGCCG
Eco47III 671 AGCGCT AGC^GCT
Eco52I 508 CGGCCG C^GGCCG
EcoT14I 68 CCATGG C^C[AT][AT]GG
FbaI 1649 TGATCA T^GATCA
FspI 1115 TGCGCA TGC^GCA
HincII 1171 GTCAAC GT[CT]^[GA]AC
HindII 1171 GTCAAC GT[CT]^[GA]AC
MroI 1268 TCCGGA T^CCGGA
MseI 1615 TTAA T^TAA
NcoI 68 CCATGG C^CATGG
PflMI 594 CCACCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 598 CACGTG CAC^GTG
PmlI 598 CACGTG CAC^GTG
SacI 1135 GAGCTC GAGCT^C
SbfI 33 CCTGCAGG CCTGCA^GG
SfiI 509 GGCCGCAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 846 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 343 CTCAAG C^T[CT][GA]AG
Sse8387I 33 CCTGCAGG CCTGCA^GG
SspBI 1349 TGTACA T^GTACA
StyI 68 CCATGG C^C[AT][AT]GG
Tru9I 1615 TTAA T^TAA
TspRI 680 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 594 CCACCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
ApaI 12 GGGCCC GGGCC^C
555 GGGCCC
BalI 692 TGGCCA TGG^CCA
962 TGGCCA
BanI 447 GGCGCC G^G[CT][GA]CC
850 GGCGCC
BbeI 447 GGCGCC GGCGC^C
850 GGCGCC
BglII 1505 AGATCT A^GATCT
1520 AGATCT
BsaAI 598 CACGTG [CT]AC^GT[GA]
1153 TACGTG
BsiEI 508 CGGCCG CG[GA][CT]^CG
870 CGACCG
BssHII 527 GCGCGC G^CGCGC
620 GCGCGC
BstAPI 860 GCATCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
917 GCACCGGCTGC
BstXI 68 CCATGGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
158 CCAACCTGATGG
HgaI 613 GCGTC GACGC(5/10)
812 GCGTC
KasI 447 GGCGCC G^GCGCC
850 GGCGCC
MluNI 692 TGGCCA TGG^CCA
962 TGGCCA
MscI 692 TGGCCA TGG^CCA
962 TGGCCA
NarI 447 GGCGCC GG^CGCC
850 GGCGCC
PstI 34 CTGCAG CTGCA^G
1582 CTGCAG
SexAI 997 ACCTGGT A^CC[AT]GGT
1214 ACCTGGT
SfaNI 860 GCATC GCATC(5/9)
1683 GCATC
SfcI 34 CTGCAG C^T[GA][CT]AG
1582 CTGCAG
SfoI 447 GGCGCC GGC^GCC
850 GGCGCC
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# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaI AvaII BanII
BbsI BbvI BcnI BglI BmyI
BpmI BpuAI BsaHI BsaJI BsgI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BssSI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PleI RsaI SapI
Sau3AI Sau96I ScrFI SmaI TaqI
TseI TthHB8I XcmI XhoII XmaCI
XmaI