Restriction map for
pFN21AE2787
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BbrPI BbvCI BciVI BclI
BfrI BlnI BsaAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspHI
BsrGI BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI DraII DraIII Eam1105I Eco47III
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI MfeI MluI MunI NcoI
NheI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
RcaI RsrII SacI SalI SbfI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI StyI
SwaI Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccI 1327 GTCTAC GT^[AC][GT]AC
AccIII 561 TCCGGA T^CCGGA
AfaI 1371 GTAC GT^AC
AflIII 776 ACATGT A^C[GA][CT]GT
AseI 1303 ATTAAT AT^TAAT
AsnI 1303 ATTAAT AT^TAAT
BcgI 1392 CGAGATCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 303 CCGGG CC^[GC]GG
BfaI 572 CTAG C^TAG
BglII 1394 AGATCT A^GATCT
BlpI 321 GCTGAGC GC^T[ACGT]AGC
BpmI 136 CTGGAG CTGGAG(16/14)
Bpu1102I 321 GCTGAGC GC^T[ACGT]AGC
BsaBI 1380 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1682 GAGACC GGTCTC(1/5)
BsaWI 561 TCCGGA [AT]^CCGG[AT]
BseAI 561 TCCGGA T^CCGGA
BseRI 1079 CTCCTC GAGGAG(10/8)
BsgI 420 CTGCAC GTGCAG(16/14)
BsiEI 65 CGGCCG CG[GA][CT]^CG
BspEI 561 TCCGGA T^CCGGA
BspLU11I 776 ACATGT A^CATGT
BsrBI 28 GAGCGG CCGCTC(-3/-3)
BsrDI 636 CATTGC GCAATG(2/0)
BstAPI 1368 GCAGTACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 831 CCAGAGTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1394 AGATCT [GA]^GATC[CT]
CelII 321 GCTGAGC GC^T[ACGT]AGC
DrdI 1153 GACAAACTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 65 CGGCCG C^GGCCG
EclXI 65 CGGCCG C^GGCCG
Eco52I 65 CGGCCG C^GGCCG
KspI 68 CCGCGG CCGC^GG
MaeI 572 CTAG C^TAG
MamI 1380 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1394 AGATCT [GA]^GATC[CT]
MroI 561 TCCGGA T^CCGGA
MslI 771 CACCGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 95 GCCGGC GCC^GGC
NciI 303 CCGGG CC^[GC]GG
NdeI 1022 CATATG CA^TATG
NgoMIV 95 GCCGGC G^CCGGC
NotI 64 GCGGCCGC GC^GGCCGC
PflMI 1541 CCATCGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1303 ATTAAT AT^TAAT
PvuII 1433 CAGCTG CAG^CTG
RsaI 1371 GTAC GT^AC
SacII 68 CCGCGG CCGC^GG
SapI 237 GCTCTTC GCTCTTC(1/4)
ScaI 1370 AGTACT AGT^ACT
SphI 347 GCATGC GCATG^C
StuI 1413 AGGCCT AGG^CCT
Van91I 1541 CCATCGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 810 CCAGAAGCGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1394 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AspHI 1123 GTGCTC G[AT]GC[AT]^C
1239 GTGCTC
AvaII 433 GGTCC G^G[AT]CC
559 GGTCC
BalI 503 TGGCCA TGG^CCA
726 TGGCCA
BanII 6 GGGCTC G[GA]GC[CT]^C
261 GGGCTC
BglI 67 GCCGCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
220 GCCAGCGAGGC
BsiHKAI 1123 GTGCTC G[AT]GC[AT]^C
1239 GTGCTC
BsmAI 1682 GAGAC GTCTC(1/5)
903 GTCTC
BsmFI 434 GTCCC GGGAC(10/14)
964 GTCCC
BspMI 338 ACCTGC ACCTGC(4/8)
1359 GCAGGT
BsrFI 95 GCCGGC [GA]^CCGG[CT]
462 ACCGGC
Cfr10I 95 GCCGGC [GA]^CCGG[CT]
462 ACCGGC
DdeI 322 CTGAG C^T[ACGT]AG
1431 CTCAG
DsaI 68 CCGCGG C^C[GA][CT]GG
1217 CCGTGG
MaeIII 667 GTCAC ^GT[ACGT]AC
1559 GTCAC
MluNI 503 TGGCCA TGG^CCA
726 TGGCCA
MscI 503 TGGCCA TGG^CCA
726 TGGCCA
PleI 854 GAGTC GAGTC(4/5)
1035 GAGTC
PstI 1094 CTGCAG CTGCA^G
1253 CTGCAG
SexAI 170 ACCTGGT A^CC[AT]GGT
542 ACCAGGT
SfaNI 1117 GATGC GCATC(5/9)
158 GCATC
SfcI 1094 CTGCAG C^T[GA][CT]AG
1253 CTGCAG
SspI 1066 AATATT AAT^ATT
1576 AATATT
TfiI 889 GATTC G^A[AT]TC
1050 GATTC
Tsp45I 667 GTCAC ^GT[GC]AC
1559 GTCAC
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# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AcsI AcyI AluI
AlwI ApoI BanI BbeI BbsI
BbvI BmyI BpuAI BsaHI BsaJI
BsiYI BslI Bsp1286I BsrI BssHII
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DpnII EaeI EarI
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeII MboI MboII
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NarI NdeII NlaIII
NlaIV NspI Sau3AI Sau96I ScrFI
SfoI TaqI Tru9I TseI Tsp509I
TspRI TthHB8I