Restriction map for
pFN21AE6747
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# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AflII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspHI AviII AvrII
BbeI BcgI BfrI BglI BglII
BlnI BsaBI BsiEI BsiHKAI BsiWI
BsmBI BspDI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRV FseI FspI HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MunI NaeI NarI NcoI
NgoMIV NheI NotI NruI NspV
PacI PflMI PinAI PmeI PshAI
PvuI RsrII SacI SacII SalI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SwaI Van91I XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccIII 14 TCCGGA T^CCGGA
AclI 424 AACGTT AA^CGTT
AcyI 2356 GGCGTC G[GA]^CG[CT]C
AocI 2259 CCTGAGG CC^T[ACGT]AGG
AspI 1516 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 2500 GGCACC G^G[CT][GA]CC
BanII 1032 GGGCTC G[GA]GC[CT]^C
BbrPI 742 CACGTG CAC^GTG
BbsI 634 GAAGAC GAAGAC(2/6)
BciVI 1795 GGATAC GTATCC(6/5)
BclI 2417 TGATCA T^GATCA
BlpI 2274 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 2274 GCTTAGC GC^T[ACGT]AGC
BpuAI 634 GAAGAC GAAGAC(2/6)
BsaAI 742 CACGTG [CT]AC^GT[GA]
BsaHI 2356 GGCGTC G[GA]^CG[CT]C
BseAI 14 TCCGGA T^CCGGA
BspEI 14 TCCGGA T^CCGGA
BspMI 1010 ACCTGC ACCTGC(4/8)
BsrBI 772 GAGCGG CCGCTC(-3/-3)
BsrFI 1349 GCCGGT [GA]^CCGG[CT]
BstAPI 1005 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 26 CCATGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2259 CCTGAGG CC^T[ACGT]AGG
CelII 2274 GCTTAGC GC^T[ACGT]AGC
Cfr10I 1349 GCCGGT [GA]^CCGG[CT]
DraIII 2134 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 2259 CCTGAGG CC^T[ACGT]AGG
EcoRI 451 GAATTC G^AATTC
EcoT22I 2235 ATGCAT ATGCA^T
FbaI 2417 TGATCA T^GATCA
Hin1I 2356 GGCGTC G[GA]^CG[CT]C
MroI 14 TCCGGA T^CCGGA
NdeI 1684 CATATG CA^TATG
NsiI 2235 ATGCAT ATGCA^T
PflFI 1516 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 742 CACGTG CAC^GTG
PmlI 742 CACGTG CAC^GTG
Psp1406I 424 AACGTT AA^CGTT
ScaI 2222 AGTACT AGT^ACT
StuI 96 AGGCCT AGG^CCT
Tth111I 1516 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 282 TCTAGA T^CTAGA
XcmI 2136 CCAGGTGGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 42 enzymes) #
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AccI 883 GTCTAC GT^[AC][GT]AC
2528 GTCTAC
AlwNI 499 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1006 CAGGACCTG
AseI 214 ATTAAT AT^TAAT
2193 ATTAAT
AsnI 214 ATTAAT AT^TAAT
2193 ATTAAT
Asp700I 1734 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2478 GAACAAATTC
BalI 702 TGGCCA TGG^CCA
1616 TGGCCA
BamHI 1106 GGATCC G^GATCC
1551 GGATCC
BbvCI 75 CCTCAGC CCTCAGC(-5/-2)
976 GCTGAGG
BfaI 181 CTAG C^TAG
283 CTAG
BsaI 22 GAGACC GGTCTC(1/5)
113 GAGACC
BsaWI 14 TCCGGA [AT]^CCGG[AT]
2051 ACCGGA
BseRI 769 GAGGAG GAGGAG(10/8)
1027 GAGGAG
BsmI 1559 GCATTC GAATGC(1/-1)
1865 GCATTC
Bsp1407I 1385 TGTACA T^GTACA
1463 TGTACA
BspHI 1067 TCATGA T^CATGA
1328 TCATGA
BspLU11I 938 ACATGT A^CATGT
1886 ACATGT
BsrDI 972 CATTGC GCAATG(2/0)
2099 CATTGC
BsrGI 1385 TGTACA T^GTACA
1463 TGTACA
Cac8I 250 GCCAGC GC[ACGT]^[ACGT]GC
2497 GCAGGC
EcoT14I 543 CCAAGG C^C[AT][AT]GG
1590 CCTTGG
FauI 803 CCCGC CCCGC(4/6)
1483 CCCGC
HaeII 79 AGCGCC [GA]GCGC^[CT]
2271 GGCGCT
HgaI 1520 GCGTC GACGC(5/10)
2357 GCGTC
HincII 1426 GTCAAC GT[CT]^[GA]AC
2341 GTTGAC
HindII 1426 GTCAAC GT[CT]^[GA]AC
2341 GTTGAC
MaeI 181 CTAG C^TAG
283 CTAG
MluNI 702 TGGCCA TGG^CCA
1616 TGGCCA
MscI 702 TGGCCA TGG^CCA
1616 TGGCCA
MspA1I 489 CAGCTG C[AC]G^C[GT]G
1546 CAGCTG
PaeR7I 310 CTCGAG C^TCGAG
1035 CTCGAG
PpuMI 4 GGGTCCC [GA]G^G[AT]CC[CT]
1007 AGGACCT
PshBI 214 ATTAAT AT^TAAT
2193 ATTAAT
PstI 49 CTGCAG CTGCA^G
1003 CTGCAG
PvuII 489 CAGCTG CAG^CTG
1546 CAGCTG
RcaI 1067 TCATGA T^CATGA
1328 TCATGA
SapI 175 GAAGAGC GCTCTTC(1/4)
42 GCTCTTC
SexAI 1751 ACCTGGT A^CC[AT]GGT
2135 ACCAGGT
SmlI 310 CTCGAG C^T[CT][GA]AG
1035 CTCGAG
SspBI 1385 TGTACA T^GTACA
1463 TGTACA
StyI 543 CCAAGG C^C[AT][AT]GG
1590 CCTTGG
XhoI 310 CTCGAG C^TCGAG
1035 CTCGAG
XmnI 1734 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2478 GAACAAATTC
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# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI AvaI AvaII
BbvI BcnI BmyI BpmI BsaJI
BsgI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstYI CfoI Cfr13I
DdeI DpnII DraII EaeI EarI
EcoNI EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII