Restriction map for
pFN21AE5240
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# NO CUT ENZYMES ( 157 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AviII AvrII BanII BbeI
BbrPI BbvCI BcgI BciVI BfaI
BfrI BglI BglII BlnI BlpI
BmyI Bpu1102I BsaAI BsaHI BsaWI
BseAI BsgI BsiHKAI BsiWI BsmBI
BsmI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrGI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraII DraIII
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HaeII HgaI Hin1I HincII HindII
HpaI KasI KpnI KspI MaeI
MaeII MfeI MluI MroI MslI
MspA1I MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI XbaI XcmI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AspI 268 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 489 GGACC G^G[AT]CC
BalI 179 TGGCCA TGG^CCA
BamHI 419 GGATCC G^GATCC
BanI 76 GGCACC G^G[CT][GA]CC
BbsI 85 GAAGAC GAAGAC(2/6)
BbvI 65 GCAGC GCAGC(8/12)
BclI 329 TGATCA T^GATCA
BcnI 633 CCGGG CC^[GC]GG
BpmI 108 CTGGAG CTGGAG(16/14)
BpuAI 85 GAAGAC GAAGAC(2/6)
BsaI 617 GAGACC GGTCTC(1/5)
BsiEI 96 CGACCG CG[GA][CT]^CG
BsmAI 617 GAGAC GTCTC(1/5)
BsrDI 115 GCAATG GCAATG(2/0)
BsrFI 491 ACCGGC [GA]^CCGG[CT]
BstBI 500 TTCGAA TT^CGAA
CfoI 101 GCGC GCG^C
Cfr10I 491 ACCGGC [GA]^CCGG[CT]
DdeI 472 CTGAG C^T[ACGT]AG
DrdI 97 GACCGCGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 179 TGGCCA [CT]^GGCC[GA]
EarI 639 GAAGAG CTCTTC(1/4)
FbaI 329 TGATCA T^GATCA
HhaI 101 GCGC GCG^C
HinP1I 101 GCGC G^CGC
HindIII 335 AAGCTT A^AGCTT
Ksp632I 639 GAAGAG CTCTTC(1/4)
MluNI 179 TGGCCA TGG^CCA
MscI 179 TGGCCA TGG^CCA
NciI 633 CCGGG CC^[GC]GG
NspV 500 TTCGAA TT^CGAA
PaeR7I 600 CTCGAG C^TCGAG
PflFI 268 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 665 CCAGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfcI 540 CTATAG C^T[GA][CT]AG
SfuI 500 TTCGAA TT^CGAA
SmlI 600 CTCGAG C^T[CT][GA]AG
TseI 65 GCAGC G^C[AT]GC
Tsp509I 212 AATT ^AATT
TspRI 592 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 268 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 665 CCAGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 600 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 100 CGCG CG^CG
102 CGCG
AvaI 581 CCCGAG C^[CT]CG[GA]G
600 CTCGAG
BsaBI 27 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
572 GATCTCGATC
BseRI 163 CTCCTC GAGGAG(10/8)
316 GAGGAG
BsoBI 581 CCCGAG C^[CT]CG[GA]G
600 CTCGAG
BstF5I 243 CATCC GGATG(2/0)
26 GGATG
BstNI 357 CCTGG CC^[AT]GG
665 CCAGG
BstUI 100 CGCG CG^CG
102 CGCG
BstYI 156 AGATCC [GA]^GATC[CT]
419 GGATCC
EcoRII 357 CCTGG ^CC[AT]GG
665 CCAGG
EcoT14I 38 CCAAGG C^C[AT][AT]GG
200 CCAAGG
Fnu4HI 65 GCAGC GC^[ACGT]GC
249 GCGGC
FokI 243 CATCC GGATG(9/13)
26 GGATG
HphI 238 GGTGA GGTGA(8/7)
167 TCACC
ItaI 65 GCAGC GC^[ACGT]GC
249 GCGGC
MaeIII 239 GTGAC ^GT[ACGT]AC
274 GTCAC
MamI 27 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
572 GATCTCGATC
MflI 156 AGATCC [GA]^GATC[CT]
419 GGATCC
MvaI 357 CCTGG CC^[AT]GG
665 CCAGG
MvnI 100 CGCG CG^CG
102 CGCG
SfaNI 27 GATGC GCATC(5/9)
346 GATGC
StyI 38 CCAAGG C^C[AT][AT]GG
200 CCAAGG
Tsp45I 239 GTGAC ^GT[GC]AC
274 GTCAC
XhoII 156 AGATCC [GA]^GATC[CT]
419 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AluI AlwI BsaJI BsiYI
BslI BsmFI BsrI BssKI Cac8I
Cfr13I DpnII FauI HaeIII HapII
HinfI HpaII MboI MboII MnlI
MseI MspI MwoI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
TaqI Tru9I TthHB8I