Restriction map for
pFN21AB8802
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BbeI BbrPI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsiHKAI BsiWI
BsmI BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BssHII BssSI Bst1107I
BstEII BstPI BstZ17I CelII Cfr10I
ClaI CpoI DraI DrdI Eam1105I
Eco47III EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeIII MamI MluI MroI MseI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SgrAI SnaBI
SpeI Sse8387I SspI StuI SwaI
TfiI Tru9I Tsp45I Tth111I XbaI
XcmI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 233 GTAGAC GT^[AC][GT]AC
AccII 366 CGCG CG^CG
AcsI 859 AAATTC [GA]^AATT[CT]
AfaI 3 GTAC GT^AC
AlwNI 619 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 606 CCTGAGG CC^T[ACGT]AGG
ApoI 859 AAATTC [GA]^AATT[CT]
AvaII 351 GGTCC G^G[AT]CC
BanI 738 GGTGCC G^G[CT][GA]CC
BbsI 132 GAAGAC GAAGAC(2/6)
BbvCI 789 CCTCAGC CCTCAGC(-5/-2)
BcgI 95 GCAATGGGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 628 GCCAGACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 132 GAAGAC GAAGAC(2/6)
Bsp1407I 2 TGTACA T^GTACA
BspMI 698 ACCTGC ACCTGC(4/8)
BsrGI 2 TGTACA T^GTACA
BstBI 945 TTCGAA TT^CGAA
BstUI 366 CGCG CG^CG
Bsu36I 606 CCTGAGG CC^T[ACGT]AGG
CfoI 86 GCGC GCG^C
EagI 482 CGGCCG C^GGCCG
EclXI 482 CGGCCG C^GGCCG
Eco52I 482 CGGCCG C^GGCCG
Eco81I 606 CCTGAGG CC^T[ACGT]AGG
EcoT14I 768 CCATGG C^C[AT][AT]GG
HhaI 86 GCGC GCG^C
HinP1I 86 GCGC G^CGC
MfeI 677 CAATTG C^AATTG
MunI 677 CAATTG C^AATTG
MvnI 366 CGCG CG^CG
NcoI 768 CCATGG C^CATGG
NspV 945 TTCGAA TT^CGAA
PaeR7I 744 CTCGAG C^TCGAG
PflMI 518 CCAATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 480 GACGGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 882 CTGCAG CTGCA^G
RsaI 3 GTAC GT^AC
SapI 273 GCTCTTC GCTCTTC(1/4)
SfcI 882 CTGCAG C^T[GA][CT]AG
SfuI 945 TTCGAA TT^CGAA
SmaI 386 CCCGGG CCC^GGG
SmlI 744 CTCGAG C^T[CT][GA]AG
SphI 812 GCATGC GCATG^C
SspBI 2 TGTACA T^GTACA
StyI 768 CCATGG C^C[AT][AT]GG
Van91I 518 CCAATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 744 CTCGAG C^TCGAG
XmaCI 386 CCCGGG C^CCGGG
XmaI 386 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
ApaI 419 GGGCCC GGGCC^C
773 GGGCCC
AvaI 386 CCCGGG C^[CT]CG[GA]G
744 CTCGAG
BalI 284 TGGCCA TGG^CCA
626 TGGCCA
BamHI 819 GGATCC G^GATCC
1014 GGATCC
BfaI 91 CTAG C^TAG
716 CTAG
BpmI 454 CTGGAG CTGGAG(16/14)
547 CTGGAG
BsgI 408 CTGCAC GTGCAG(16/14)
778 CTGCAC
BsiEI 482 CGGCCG CG[GA][CT]^CG
954 CGGTCG
BsmAI 478 GAGAC GTCTC(1/5)
487 GTCTC
BsmBI 486 CGTCTC CGTCTC(1/5)
478 GAGACG
BsmFI 32 GGGAC GGGAC(10/14)
251 GGGAC
BsoBI 386 CCCGGG C^[CT]CG[GA]G
744 CTCGAG
BsrDI 95 GCAATG GCAATG(2/0)
1008 GCAATG
BsrI 239 ACTGG ACTGG(1/-1)
996 CCAGT
BstAPI 401 GCATCGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
959 GCAGGAGTTGC
BstF5I 429 CATCC GGATG(2/0)
641 GGATG
BstXI 383 CCACCCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
996 CCAGTCTGATGG
BstYI 819 GGATCC [GA]^GATC[CT]
1014 GGATCC
DraIII 364 CACGCGGTG CAC[ACGT][ACGT][ACGT]^GTG
411 CACCCTGTG
DsaI 173 CCGTGG C^C[GA][CT]GG
768 CCATGG
EarI 274 CTCTTC CTCTTC(1/4)
931 GAAGAG
FokI 429 CATCC GGATG(9/13)
641 GGATG
HinfI 511 GAGTC G^A[ACGT]TC
719 GACTC
Ksp632I 274 CTCTTC CTCTTC(1/4)
931 GAAGAG
MaeI 91 CTAG C^TAG
716 CTAG
MaeII 231 ACGT A^CGT
280 ACGT
MflI 819 GGATCC [GA]^GATC[CT]
1014 GGATCC
MluNI 284 TGGCCA TGG^CCA
626 TGGCCA
MscI 284 TGGCCA TGG^CCA
626 TGGCCA
NspI 691 ACATGC [GA]CATG^[CT]
812 GCATGC
PleI 719 GACTC GAGTC(4/5)
511 GAGTC
PvuII 544 CAGCTG CAG^CTG
622 CAGCTG
XhoII 819 GGATCC [GA]^GATC[CT]
1014 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AluI AlwI BanII BbvI
BcnI BmyI BsaJI BsiYI BslI
Bsp1286I BssKI BstNI Cac8I Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII Fnu4HI HaeIII HapII HpaII
HphI ItaI MboI MboII MnlI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI TaqI TseI
Tsp509I TspRI TthHB8I