Restriction map for
pFN21AE0806
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BbeI
BbrPI BbsI BbvCI BciVI BfaI
BfrI BglI BlnI BpuAI BsaAI
BsaHI BsaI BseAI BseRI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
ClaI CpoI DraI DraIII EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FauI FseI FspI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MaeII MaeIII MfeI
MluI MroI MunI MvnI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SmaI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI Tsp45I Tth111I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 367 GTAGAC GT^[AC][GT]AC
AcsI 522 AAATTT [GA]^AATT[CT]
ApaI 1074 GGGCCC GGGCC^C
ApoI 522 AAATTT [GA]^AATT[CT]
AspHI 988 GAGCTC G[AT]GC[AT]^C
BamHI 752 GGATCC G^GATCC
BanI 8 GGCACC G^G[CT][GA]CC
BcgI 977 GCACGAGCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 381 CCGGG CC^[GC]GG
BglII 413 AGATCT A^GATCT
BlpI 620 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 620 GCTCAGC GC^T[ACGT]AGC
BsaWI 1045 TCCGGT [AT]^CCGG[AT]
BsiHKAI 988 GAGCTC G[AT]GC[AT]^C
BsmAI 651 GAGAC GTCTC(1/5)
BsrBI 807 CCGCTC CCGCTC(-3/-3)
BsrDI 131 GCAATG GCAATG(2/0)
BssSI 978 CACGAG CACGAG(-5/-1)
BstAPI 9 GCACCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 620 GCTCAGC GC^T[ACGT]AGC
CfoI 1019 GCGC GCG^C
DraII 998 GGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 70 GACTTCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 310 CTCTTC CTCTTC(1/4)
EcoO109I 998 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 1054 CCATGG C^C[AT][AT]GG
HaeII 1018 GGCGCT [GA]GCGC^[CT]
HgaI 983 GCGTC GACGC(5/10)
HhaI 1019 GCGC GCG^C
HinP1I 1019 GCGC G^CGC
HinfI 3 GAATC G^A[ACGT]TC
Ksp632I 310 CTCTTC CTCTTC(1/4)
MseI 1033 TTAA T^TAA
MspA1I 930 CCGCTG C[AC]G^C[GT]G
NaeI 299 GCCGGC GCC^GGC
NciI 381 CCGGG CC^[GC]GG
NcoI 1054 CCATGG C^CATGG
NgoMIV 299 GCCGGC G^CCGGC
PpuMI 998 GGGACCC [GA]G^G[AT]CC[CT]
PstI 16 CTGCAG CTGCA^G
SacI 988 GAGCTC GAGCT^C
SapI 309 GCTCTTC GCTCTTC(1/4)
SfcI 16 CTGCAG C^T[GA][CT]AG
SgrAI 92 CGCCGGTG C[GA]^CCGG[CT]G
SmlI 166 CTCAAG C^T[CT][GA]AG
StyI 1054 CCATGG C^C[AT][AT]GG
TaqI 986 TCGA T^CGA
TfiI 3 GAATC G^A[AT]TC
Tru9I 1033 TTAA T^TAA
TthHB8I 986 TCGA T^CGA
XcmI 572 CCATGTTCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 804 GTAC GT^AC
879 GTAC
AlwNI 209 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
540 CAGCATCTG
BalI 707 TGGCCA TGG^CCA
1037 TGGCCA
BclI 227 TGATCA T^GATCA
923 TGATCA
BsaBI 498 GATGCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
753 GATCCGCATC
BsgI 772 CTGCAC GTGCAG(16/14)
528 GTGCAG
BsrI 275 ACTGG ACTGG(1/-1)
558 CCAGT
BstYI 413 AGATCT [GA]^GATC[CT]
752 GGATCC
DdeI 621 CTCAG C^T[ACGT]AG
838 CTCAG
DsaI 872 CCGTGG C^C[GA][CT]GG
1054 CCATGG
EaeI 707 TGGCCA [CT]^GGCC[GA]
1037 TGGCCA
FbaI 227 TGATCA T^GATCA
923 TGATCA
MamI 498 GATGCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
753 GATCCGCATC
MflI 413 AGATCT [GA]^GATC[CT]
752 GGATCC
MluNI 707 TGGCCA TGG^CCA
1037 TGGCCA
MscI 707 TGGCCA TGG^CCA
1037 TGGCCA
NspI 666 GCATGC [GA]CATG^[CT]
860 GCATGC
RsaI 804 GTAC GT^AC
879 GTAC
SphI 666 GCATGC GCATG^C
860 GCATGC
TspRI 209 CAGTG CA[GC]TG[ACGT][ACGT]^
559 CAGTG
XhoII 413 AGATCT [GA]^GATC[CT]
752 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AluI AlwI AvaII BanII
BbvI BmyI BpmI BsaJI BsiYI
BslI BsmFI Bsp1286I BsrFI BssKI
BstF5I BstNI Cac8I Cfr10I Cfr13I
DpnII EcoRII Fnu4HI FokI HaeIII
HapII HpaII HphI ItaI MboI
MboII MnlI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI TseI Tsp509I