Restriction map for
pFN21AE4023
##################################
# NO CUT ENZYMES ( 134 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BalI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaI BsaWI BseAI BseRI BsgI
BsiWI BsmAI BsmBI BsmFI BsmI
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrI BssHII Bst1107I BstAPI
BstBI BstXI BstZ17I CelII ClaI
DraI DrdI Eam1105I EarI EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI HincII HindII HindIII HpaI
KasI KpnI Ksp632I MaeI MfeI
MluI MluNI MroI MscI MseI
MunI NarI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI SacI SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tru9I Tsp509I TspRI Van91I
XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccII 364 CGCG CG^CG
AlwNI 469 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 580 AGCGCT AGC^GCT
ApaI 14 GGGCCC GGGCC^C
AspI 319 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 40 GGTCC G^G[AT]CC
BamHI 368 GGATCC G^GATCC
BpmI 448 CTGGAG CTGGAG(16/14)
BsaBI 63 GATCGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 221 CGGCCG CG[GA][CT]^CG
Bsp1407I 113 TGTACA T^GTACA
BspHI 299 TCATGA T^CATGA
BsrGI 113 TGTACA T^GTACA
BssSI 277 CTCGTG CACGAG(-5/-1)
BstEII 198 GGTGACC G^GT[ACGT]ACC
BstF5I 3 GGATG GGATG(2/0)
BstPI 198 GGTGACC G^GT[ACGT]ACC
BstUI 364 CGCG CG^CG
CpoI 39 CGGTCCG CG^G[AT]CCG
DraIII 274 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 221 CGGCCG C^GGCCG
EclXI 221 CGGCCG C^GGCCG
Eco47III 580 AGCGCT AGC^GCT
Eco52I 221 CGGCCG C^GGCCG
EcoNI 402 CCTGAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 198 GGTGACC G^GT[ACGT]ACC
FauI 365 GCGGG CCCGC(4/6)
FokI 3 GGATG GGATG(9/13)
HinfI 303 GAATC G^A[ACGT]TC
KspI 363 CCGCGG CCGC^GG
MamI 63 GATCGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 459 CACCTACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 364 CGCG CG^CG
NaeI 614 GCCGGC GCC^GGC
NcoI 374 CCATGG C^CATGG
NgoMIV 614 GCCGGC G^CCGGC
NspI 464 ACATGC [GA]CATG^[CT]
PflFI 319 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 299 TCATGA T^CATGA
RsrII 39 CGGTCCG CG^G[AT]CCG
SacII 363 CCGCGG CCGC^GG
SfaNI 4 GATGC GCATC(5/9)
SmaI 10 CCCGGG CCC^GGG
SspBI 113 TGTACA T^GTACA
TaqI 158 TCGA T^CGA
TfiI 303 GAATC G^A[AT]TC
Tth111I 319 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 158 TCGA T^CGA
XmaCI 10 CCCGGG C^CCGGG
XmaI 10 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AatII 295 GACGTC GACGT^C
547 GACGTC
AcyI 295 GACGTC G[GA]^CG[CT]C
547 GACGTC
AfaI 114 GTAC GT^AC
165 GTAC
AocI 343 CCTGAGG CC^T[ACGT]AGG
402 CCTGAGG
AvaI 10 CCCGGG C^[CT]CG[GA]G
133 CCCGAG
BbsI 232 GAAGAC GAAGAC(2/6)
550 GTCTTC
BglI 7 GCCCCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
565 GCCAAGGTGGC
BpuAI 232 GAAGAC GAAGAC(2/6)
550 GTCTTC
BsaHI 295 GACGTC G[GA]^CG[CT]C
547 GACGTC
BsoBI 10 CCCGGG C^[CT]CG[GA]G
133 CCCGAG
BsrFI 242 ACCGGC [GA]^CCGG[CT]
614 GCCGGC
BstYI 368 GGATCC [GA]^GATC[CT]
491 AGATCC
Bsu36I 343 CCTGAGG CC^T[ACGT]AGG
402 CCTGAGG
Cfr10I 242 ACCGGC [GA]^CCGG[CT]
614 GCCGGC
DsaI 363 CCGCGG C^C[GA][CT]GG
374 CCATGG
EaeI 170 CGGCCA [CT]^GGCC[GA]
221 CGGCCG
Eco81I 343 CCTGAGG CC^T[ACGT]AGG
402 CCTGAGG
EcoT14I 374 CCATGG C^C[AT][AT]GG
566 CCAAGG
Hin1I 295 GACGTC G[GA]^CG[CT]C
547 GACGTC
HphI 198 GGTGA GGTGA(8/7)
458 TCACC
MflI 368 GGATCC [GA]^GATC[CT]
491 AGATCC
PvuII 421 CAGCTG CAG^CTG
445 CAGCTG
RsaI 114 GTAC GT^AC
165 GTAC
StyI 374 CCATGG C^C[AT][AT]GG
566 CCAAGG
XhoII 368 GGATCC [GA]^GATC[CT]
491 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AluI AlwI AspHI BanII
BbvI BcnI BmyI BsaJI BsiHKAI
BsiYI BslI Bsp1286I BssKI BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII Fnu4HI HaeII
HaeIII HapII HhaI HinP1I HpaII
ItaI MaeII MaeIII MboI MboII
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI TseI Tsp45I