Restriction map for
pFN21AE9613
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
Acc65I AccII AclI AflII AflIII
AgeI AlwNI AocI AscI AseI
AsnI Asp700I Asp718I AviII AvrII
BalI BamHI BbrPI BbsI BcgI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaI BsiWI BsmBI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstUI BstXI BstYI
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII EaeI EagI EarI
EclXI Eco52I Eco81I EcoNI EcoRV
FbaI FseI FspI HgaI HindIII
HpaI KpnI Ksp632I KspI MaeI
MamI MfeI MflI MluI MluNI
MscI MseI MunI MvnI NaeI
NdeI NgoMIV NheI NotI NruI
NspI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
XbaI XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 610 GACGTC GACGT^C
AccI 793 GTATAC GT^[AC][GT]AC
AcsI 900 GAATTC [GA]^AATT[CT]
AhdI 217 GACTACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 736 GTGCAC G^TGCAC
Aor51HI 143 AGCGCT AGC^GCT
ApaI 862 GGGCCC GGGCC^C
ApaLI 736 GTGCAC G^TGCAC
ApoI 900 GAATTC [GA]^AATT[CT]
AspEI 217 GACTACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 493 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 530 CCCGGG C^[CT]CG[GA]G
BanI 464 GGCGCC G^G[CT][GA]CC
BbeI 464 GGCGCC GGCGC^C
BbvCI 580 GCTGAGG CCTCAGC(-5/-2)
BciVI 209 GGATAC GTATCC(6/5)
BpmI 573 CTGGAG CTGGAG(16/14)
BsgI 368 CTGCAC GTGCAG(16/14)
BsiEI 871 CGGTCG CG[GA][CT]^CG
BsmAI 165 GAGAC GTCTC(1/5)
BsoBI 530 CCCGGG C^[CT]CG[GA]G
Bst1107I 793 GTATAC GTA^TAC
BstEII 420 GGTGACC G^GT[ACGT]ACC
BstPI 420 GGTGACC G^GT[ACGT]ACC
BstZ17I 793 GTATAC GTA^TAC
DrdI 21 GACTTTCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 217 GACTACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 143 AGCGCT AGC^GCT
EcoO65I 420 GGTGACC G^GT[ACGT]ACC
EcoRI 900 GAATTC G^AATTC
EcoT22I 486 ATGCAT ATGCA^T
HincII 18 GTTGAC GT[CT]^[GA]AC
HindII 18 GTTGAC GT[CT]^[GA]AC
KasI 464 GGCGCC G^GCGCC
MaeII 611 ACGT A^CGT
MslI 739 CACAAGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 307 CAGCGG C[AC]G^C[GT]G
NarI 464 GGCGCC GG^CGCC
NcoI 588 CCATGG C^CATGG
NsiI 486 ATGCAT ATGCA^T
PflFI 493 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 475 GAGCTC GAGCT^C
SfcI 219 CTACAG C^T[GA][CT]AG
SfoI 464 GGCGCC GGC^GCC
SmaI 530 CCCGGG CCC^GGG
TaqI 73 TCGA T^CGA
Tth111I 493 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 73 TCGA T^CGA
XmaCI 530 CCCGGG C^CCGGG
XmaI 530 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AccIII 287 TCCGGA T^CCGGA
635 TCCGGA
AcyI 464 GGCGCC G[GA]^CG[CT]C
610 GACGTC
AfaI 312 GTAC GT^AC
411 GTAC
AlwI 47 GGATC GGATC(4/5)
778 GGATC
BanII 475 GAGCTC G[GA]GC[CT]^C
862 GGGCCC
BbvI 540 GCTGC GCAGC(8/12)
921 GCTGC
BcnI 530 CCCGG CC^[GC]GG
531 CCGGG
BsaHI 464 GGCGCC G[GA]^CG[CT]C
610 GACGTC
BsaWI 287 TCCGGA [AT]^CCGG[AT]
635 TCCGGA
BseAI 287 TCCGGA T^CCGGA
635 TCCGGA
BsmFI 704 GGGAC GGGAC(10/14)
649 GTCCC
BspEI 287 TCCGGA T^CCGGA
635 TCCGGA
BspMI 655 ACCTGC ACCTGC(4/8)
789 GCAGGT
CfoI 144 GCGC GCG^C
465 GCGC
DpnII 48 GATC ^GATC
779 GATC
DraII 497 GGGTCCT [GA]G^G[ACGT]CC[CT]
647 AGGTCCC
DsaI 480 CCACGG C^C[GA][CT]GG
588 CCATGG
EcoO109I 497 GGGTCCT [GA]G^G[ACGT]CC[CT]
647 AGGTCCC
EcoT14I 449 CCAAGG C^C[AT][AT]GG
588 CCATGG
FauI 248 CCCGC CCCGC(4/6)
454 GCGGG
Fnu4HI 540 GCTGC GC^[ACGT]GC
921 GCTGC
HaeII 143 AGCGCT [GA]GCGC^[CT]
464 GGCGCC
HhaI 144 GCGC GCG^C
465 GCGC
Hin1I 464 GGCGCC G[GA]^CG[CT]C
610 GACGTC
HinP1I 144 GCGC G^CGC
465 GCGC
ItaI 540 GCTGC GC^[ACGT]GC
921 GCTGC
MboI 48 GATC ^GATC
779 GATC
MroI 287 TCCGGA T^CCGGA
635 TCCGGA
NciI 530 CCCGG CC^[GC]GG
531 CCGGG
NdeII 48 GATC ^GATC
779 GATC
PflMI 426 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
588 CCATGGCCTGG
PpuMI 497 GGGTCCT [GA]G^G[AT]CC[CT]
647 AGGTCCC
RsaI 312 GTAC GT^AC
411 GTAC
Sau3AI 48 GATC ^GATC
779 GATC
SfaNI 436 GATGC GCATC(5/9)
485 GATGC
StyI 449 CCAAGG C^C[AT][AT]GG
588 CCATGG
TfiI 191 GAATC G^A[AT]TC
202 GAATC
TseI 540 GCTGC G^C[AT]GC
921 GCTGC
Tsp45I 421 GTGAC ^GT[GC]AC
608 GTGAC
Tsp509I 344 AATT ^AATT
901 AATT
Van91I 426 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
588 CCATGGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AluI AspHI AvaII BmyI
BsaJI BseRI BsiHKAI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI EcoRII FokI
HaeIII HapII HinfI HpaII HphI
MaeIII MboII MnlI MspI MvaI
MwoI NlaIII NlaIV PleI Sau96I
ScrFI TspRI