Restriction map for
pFN21AE1764
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AhdI Alw44I AlwNI
AocI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BsaAI BsaBI
BsaI BseAI BsgI BsiEI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrGI BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco52I
Eco81I EcoO65I EcoRV EcoT22I FbaI
FseI FspI HindIII KasI KpnI
KspI MaeI MamI MfeI MluI
MroI MspA1I MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcsI 175 GAATTC [GA]^AATT[CT]
AgeI 764 ACCGGT A^CCGGT
Aor51HI 593 AGCGCT AGC^GCT
ApoI 175 GAATTC [GA]^AATT[CT]
AvaI 72 CTCGGG C^[CT]CG[GA]G
BalI 45 TGGCCA TGG^CCA
BbsI 124 GAAGAC GAAGAC(2/6)
BbvI 189 GCAGC GCAGC(8/12)
BcnI 59 CCCGG CC^[GC]GG
BglI 431 GCCAGCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 750 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 750 GCTCAGC GC^T[ACGT]AGC
BpuAI 124 GAAGAC GAAGAC(2/6)
BsaWI 764 ACCGGT [AT]^CCGG[AT]
BseRI 628 CTCCTC GAGGAG(10/8)
BsoBI 72 CTCGGG C^[CT]CG[GA]G
BsrBI 34 GAGCGG CCGCTC(-3/-3)
BsrFI 764 ACCGGT [GA]^CCGG[CT]
BssHII 21 GCGCGC G^CGCGC
CelII 750 GCTCAGC GC^T[ACGT]AGC
Cfr10I 764 ACCGGT [GA]^CCGG[CT]
Eco47III 593 AGCGCT AGC^GCT
EcoNI 642 CCTGAACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 175 GAATTC G^AATTC
EcoT14I 83 CCAAGG C^C[AT][AT]GG
FauI 220 CCCGC CCCGC(4/6)
HaeII 593 AGCGCT [GA]GCGC^[CT]
HincII 445 GTTAAC GT[CT]^[GA]AC
HindII 445 GTTAAC GT[CT]^[GA]AC
HinfI 505 GATTC G^A[ACGT]TC
HpaI 445 GTTAAC GTT^AAC
MluNI 45 TGGCCA TGG^CCA
MscI 45 TGGCCA TGG^CCA
MslI 456 CATTCAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 59 CCCGG CC^[GC]GG
PinAI 764 ACCGGT A^CCGGT
SapI 589 GAAGAGC GCTCTTC(1/4)
StyI 83 CCAAGG C^C[AT][AT]GG
TaqI 246 TCGA T^CGA
TfiI 505 GATTC G^A[AT]TC
TseI 189 GCAGC G^C[AT]GC
Tsp45I 784 GTCAC ^GT[GC]AC
Tsp509I 176 AATT ^AATT
TthHB8I 246 TCGA T^CGA
XcmI 101 CCAAGATCCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 54 GACGCC G[GA]^CG[CT]C
127 GACGCC
AspHI 516 GTGCTC G[AT]GC[AT]^C
748 GTGCTC
AvaII 284 GGTCC G^G[AT]CC
553 GGACC
BsaHI 54 GACGCC G[GA]^CG[CT]C
127 GACGCC
BsiHKAI 516 GTGCTC G[AT]GC[AT]^C
748 GTGCTC
BsmFI 65 GGGAC GGGAC(10/14)
92 GGGAC
BsrI 296 ACTGG ACTGG(1/-1)
539 CCAGT
BstYI 104 AGATCC [GA]^GATC[CT]
348 GGATCT
Cac8I 21 GCGCGC GC[ACGT]^[ACGT]GC
431 GCCAGC
DraII 283 GGGTCCT [GA]G^G[ACGT]CC[CT]
552 AGGACCC
EaeI 45 TGGCCA [CT]^GGCC[GA]
232 CGGCCA
EarI 136 CTCTTC CTCTTC(1/4)
589 GAAGAG
EcoO109I 283 GGGTCCT [GA]G^G[ACGT]CC[CT]
552 AGGACCC
Hin1I 54 GACGCC G[GA]^CG[CT]C
127 GACGCC
HphI 314 TCACC GGTGA(8/7)
785 TCACC
Ksp632I 136 CTCTTC CTCTTC(1/4)
589 GAAGAG
MaeIII 424 GTAAC ^GT[ACGT]AC
784 GTCAC
MflI 104 AGATCC [GA]^GATC[CT]
348 GGATCT
MseI 446 TTAA T^TAA
496 TTAA
PpuMI 283 GGGTCCT [GA]G^G[AT]CC[CT]
552 AGGACCC
SfaNI 490 GATGC GCATC(5/9)
366 GCATC
Tru9I 446 TTAA T^TAA
496 TTAA
TspRI 15 CAGTG CA[GC]TG[ACGT][ACGT]^
394 CAGTG
XhoII 104 AGATCC [GA]^GATC[CT]
348 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
BanI BanII BmyI BpmI BsaJI
BsiYI BslI Bsp1286I BssKI BstF5I
BstNI BstUI CfoI Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HpaII
ItaI MaeII MboI MboII MnlI
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI