Restriction map for
pFN21AE2014
##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
Acc65I AccIII AcsI AflII AgeI
AocI ApaI ApoI AscI AseI
AsnI Asp718I AspI AvrII BamHI
BcgI BfrI BglI BlnI BlpI
Bpu1102I BsaWI BseAI BsgI BsiWI
BsmBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraII Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV FseI HgaI
HindIII HpaI KpnI MluI MroI
NdeI NheI NotI NruI NspV
PacI PflFI PinAI PmeI PpuMI
PshAI PshBI PvuI RsrII SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AatII 1603 GACGTC GACGT^C
AclI 840 AACGTT AA^CGTT
AhdI 1013 GACGGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 1584 GATCC GGATC(4/5)
AlwNI 500 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 878 AGCGCT AGC^GCT
Asp700I 406 GAAATAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1013 GACGGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 768 GGACC G^G[AT]CC
AviII 297 TGCGCA TGC^GCA
BalI 1439 TGGCCA TGG^CCA
BanI 748 GGCGCC G^G[CT][GA]CC
BbeI 748 GGCGCC GGCGC^C
BbrPI 894 CACGTG CAC^GTG
BbvCI 125 CCTCAGC CCTCAGC(-5/-2)
BciVI 1179 GGATAC GTATCC(6/5)
BclI 215 TGATCA T^GATCA
BglII 1566 AGATCT A^GATCT
BsaBI 1683 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 325 GAATGC GAATGC(1/-1)
BspHI 522 TCATGA T^CATGA
DraI 1333 TTTAAA TTT^AAA
DraIII 792 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 225 GACCATGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1013 GACGGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 878 AGCGCT AGC^GCT
EcoT14I 1694 CCATGG C^C[AT][AT]GG
EcoT22I 327 ATGCAT ATGCA^T
FauI 55 CCCGC CCCGC(4/6)
FbaI 215 TGATCA T^GATCA
FspI 297 TGCGCA TGC^GCA
HincII 1025 GTCGAC GT[CT]^[GA]AC
HindII 1025 GTCGAC GT[CT]^[GA]AC
KasI 748 GGCGCC G^GCGCC
KspI 77 CCGCGG CCGC^GG
MamI 1683 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1343 CAATTG C^AATTG
MluNI 1439 TGGCCA TGG^CCA
MscI 1439 TGGCCA TGG^CCA
MunI 1343 CAATTG C^AATTG
NaeI 58 GCCGGC GCC^GGC
NarI 748 GGCGCC GG^CGCC
NcoI 1694 CCATGG C^CATGG
NgoMIV 58 GCCGGC G^CCGGC
NlaIV 748 GGCGCC GG[ACGT]^[ACGT]CC
NsiI 327 ATGCAT ATGCA^T
PaeR7I 1707 CTCGAG C^TCGAG
PflMI 600 CCAATATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 894 CACGTG CAC^GTG
PmlI 894 CACGTG CAC^GTG
Psp1406I 840 AACGTT AA^CGTT
RcaI 522 TCATGA T^CATGA
SacII 77 CCGCGG CCGC^GG
SalI 1025 GTCGAC G^TCGAC
SfoI 748 GGCGCC GGC^GCC
SmlI 1707 CTCGAG C^T[CT][GA]AG
StyI 1694 CCATGG C^C[AT][AT]GG
Van91I 600 CCAATATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1362 TCTAGA T^CTAGA
XhoI 1707 CTCGAG C^TCGAG
XmnI 406 GAAATAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccI 872 GTCTAC GT^[AC][GT]AC
1025 GTCGAC
AccII 36 CGCG CG^CG
78 CGCG
AcyI 748 GGCGCC G[GA]^CG[CT]C
1603 GACGTC
AfaI 912 GTAC GT^AC
1159 GTAC
AflIII 895 ACGTGT A^C[GA][CT]GT
1061 ACACGT
AvaI 925 CCCGAG C^[CT]CG[GA]G
1707 CTCGAG
BbsI 1323 GAAGAC GAAGAC(2/6)
276 GTCTTC
BcnI 83 CCGGG CC^[GC]GG
1658 CCCGG
BpuAI 1323 GAAGAC GAAGAC(2/6)
276 GTCTTC
BsaAI 894 CACGTG [CT]AC^GT[GA]
1062 CACGTA
BsaHI 748 GGCGCC G[GA]^CG[CT]C
1603 GACGTC
BsiEI 80 CGGCCG CG[GA][CT]^CG
192 CGGCCG
BsoBI 925 CCCGAG C^[CT]CG[GA]G
1707 CTCGAG
BsrDI 511 GCAATG GCAATG(2/0)
662 GCAATG
BsrFI 58 GCCGGC [GA]^CCGG[CT]
908 GCCGGT
BstF5I 831 CATCC GGATG(2/0)
1006 GGATG
BstUI 36 CGCG CG^CG
78 CGCG
BstYI 1566 AGATCT [GA]^GATC[CT]
1583 AGATCC
Cfr10I 58 GCCGGC [GA]^CCGG[CT]
908 GCCGGT
Cfr13I 768 GGACC G^G[ACGT]CC
1755 GGGCC
DsaI 77 CCGCGG C^C[GA][CT]GG
1694 CCATGG
EagI 80 CGGCCG C^GGCCG
192 CGGCCG
EarI 1719 CTCTTC CTCTTC(1/4)
1192 GAAGAG
EclXI 80 CGGCCG C^GGCCG
192 CGGCCG
Eco52I 80 CGGCCG C^GGCCG
192 CGGCCG
FokI 831 CATCC GGATG(9/13)
1006 GGATG
Hin1I 748 GGCGCC G[GA]^CG[CT]C
1603 GACGTC
Ksp632I 1719 CTCTTC CTCTTC(1/4)
1192 GAAGAG
MflI 1566 AGATCT [GA]^GATC[CT]
1583 AGATCC
MvnI 36 CGCG CG^CG
78 CGCG
NciI 83 CCGGG CC^[GC]GG
1658 CCCGG
NspI 658 ACATGC [GA]CATG^[CT]
1029 ACATGC
PstI 1510 CTGCAG CTGCA^G
1570 CTGCAG
RsaI 912 GTAC GT^AC
1159 GTAC
SacI 31 GAGCTC GAGCT^C
1456 GAGCTC
Sau96I 768 GGACC G^G[ACGT]CC
1755 GGGCC
XcmI 596 CCAACCAATATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
999 CCACATAGGATGTGG
XhoII 1566 AGATCT [GA]^GATC[CT]
1583 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AluI Alw44I ApaLI AspHI
BanII BbvI BfaI BmyI BpmI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstNI Cac8I CfoI DdeI
DpnII EaeI EcoRII Fnu4HI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII PleI PvuII Sau3AI
ScrFI SfaNI SfcI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I